Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Cytochrome P450 1A1 (Cyp1a1)

Recombinant Mouse Cytochrome P450 1A1 (Cyp1a1)

  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A1(Cyp1a1),Yeast
  • 货号：
    CSB-YP006395MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A1(Cyp1a1),Yeast
  • 货号：
    CSB-EP006395MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A1(Cyp1a1),Yeast
  • 货号：
    CSB-EP006395MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A1(Cyp1a1),Yeast
  • 货号：
    CSB-BP006395MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cytochrome P450 1A1(Cyp1a1),Yeast
  • 货号：
    CSB-MP006395MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp1a1
  • Uniprot No.：
  • 别名：
    Cyp1a1; Cyp1a-1Cytochrome P450 1A1; EC 1.14.14.1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; Hydroperoxy icosatetraenoate dehydratase; EC 4.2.1.152
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-524
  • 氨基酸序列
    MPSMYGLPAF VSATELLLAV TVFCLGFWVV RATRTWVPKG LKTPPGPWGL PFIGHMLTVG KNPHLSLTRL SQQYGDVLQI RIGSTPVVVL SGLNTIKQAL VRQGDDFKGR PDLYSFTLIT NGKSMTFNPD SGPVWAARRR LAQNALKSFS IASDPTSASS CYLEEHVSKE ANYLVSKLQK VMAEVGHFDP YKYLVVSVAN VICAICFGQR YDHDDQELLS IVNLSNEFGE VTGSGYPADF IPVLRYLPNS SLDAFKDLND KFYSFMKKLI KEHYRTFEKG HIRDITDSLI EHCQDRKLDE NANVQLSDDK VITIVLDLFG AGFDTVTTAI SWSLMYLVTN PRVQRKIQEE LDTVIGRDRQ PRLSDRPQLP YLEAFILETF RHSSFVPFTI PHSTTRDTSL NGFYIPKGCC VFVNQWQVNH DRELWGDPNE FRPERFLTPS GTLDKRLSEK VTLFGLGKRK CIGETIGRSE VFLFLAILLQ QIEFKVSPGE KVDMTPTYGL TLKHARCEHF QVQMRSSGPQ HLQA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).
  • 基因功能参考文献：
    1. Miroestrol suppressed beta-naphthoflavone increases in CYP1A1. PMID: 26893163
    2. up-regulated expression during benzo(a)pyrene induced pulmonary carcinogenesis PMID: 26655880
    3. Taken together, CAPE decreases 3-MC-mediated CYP1A1 expression, and this inhibitory response is associated with inhibition of AhR and HIF-1alpha induction. PMID: 26296470
    4. Our results support the hypothesis that CYP1A1 protects against hyperoxic lung injury by decreasing oxidative stress. PMID: 24893714
    5. Suggest an important role for CYP1A enzymes in the gender-specific modulation of hyperoxic lung injury. PMID: 25703676
    6. Hypertension fails to disrupt white matter integrity in young or aged Fisher (F44) Cyp1a1Ren2 transgenic rats. PMID: 25407269
    7. CYP1A1 contributes to nitric oxide bioavailability and blood pressure regulation mediated by dietary omega-3 polyunsaturated fatty acids. PMID: 25316121
    8. Suggest that CYP1A induction by coplanar polychlorinated/brominated biphenyls depends on AhR transcriptional activity and not on AhR expression. PMID: 25068733
    9. Identify hepatic proteins necessary for the AHR-dependent induction of CYP1A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin. PMID: 23997114
    10. In livers of HRN mice Cyp1a1, cytochrome b5 and mEH can effectively activate BaP to DNA binding species, even in the presence of very low amounts of P450 oxidoreductase. PMID: 24530354
    11. Reactive oxygen species production contributes to benzo[a]pyrene (BaP)-exacerbated atherosclerosis and CYP1A1 plays a protective role against oral BaP toxicity in aorta PMID: 24394547
    12. harmine and harmaline are promising candidate to inhibit TCDD-mediated induction of Cyp1a1 in mice hepatic and extrahepatic tissues PMID: 23509697
    13. Regular consumption of green and white tea exerted protection against benzo(a)pyrene induced toxicity as it modulates the CYP1B1, CYP1A1 enzymes, resulting in reduction of BaPDE-DNA adducts formation. PMID: 22960141
    14. These data suggest that CYP1A1 metabolizes n-3 polyunsaturated fatty acids to vasodilators in vivo and the loss of these vasodilators may lead to increases in blood pressure. PMID: 22995157
    15. the presence of ligands for TLR2 of bacterial origin seems to be crucial for detoxication of luminal carcinogens by CYP1A1 in the intestine. PMID: 22442665
    16. Alternaria mycotoxins alternariol and alternariol methyl ether induce cytochrome P450 1A1 and apoptosis in murine hepatoma cells dependent on the aryl hydrocarbon receptor. PMID: 22120949
    17. prolonged induction of AHR activity through inhibition of CYP1 disturbs feedback regulation of FICZ levels, with potential detrimental consequences PMID: 22392998
    18. Data show that SZME3 induced an up-regulation of both Cyp1a1 and Cyp2e1 genes in both genders. PMID: 21471540
    19. Increase of CYP1A1-mRNA was due to nonmuscle myosin II inhibition. PMID: 21216307
    20. Data show that AIP is not required for the induction of the Cyp1a1 and Cyp1a2 genes. PMID: 20829355
    21. We conclude that small intestine CYP1A1, and not liver CYP1A1, is critically important in oral benzo(a)pyrene detoxication PMID: 20371670
    22. pyridoxine-deficiency results in reversable increase in hepatic CYP1A1 activity PMID: 19637937
    23. Induction of cytochrome P450 1A1 gene expression by a vitamin K3 analog in mouse hepatoma Hepa-1c1c7 cells. PMID: 11710520
    24. reduced availability of heme limits the incorporation of P450s into brain endoplasmic reticulum. PMID: 12391268
    25. CYP1A2 contributes to the up-regulation of CYP1A1 in liver, but not lung, by a novel mechanism, presumably involving a CYP1A2-dependent persistent metabolite. PMID: 12438513
    26. 3'-methoxy-4'-nitroflavone activates the Ah receptor to recognize and interact with the same dioxin responsive elements as TCDD PMID: 12859983
    27. Such incomplete resolution of this complex locus occurred progressively with age in germ cells of male mice; the conditional Cyp1a1 gene was recovered in offspring from mice containing the targeted Cyp1a1 allele and the Cre recombinase transgene. PMID: 14637164
    28. Together these results indicate that TCDD activates the MEK-1/p44/p42 MAPK pathway, which in turn activates AhR and so facilitates binding of AhR to the cyp1a1 DRE. PMID: 15313166
    29. 6-MCDF exhibits agonist activity with respect to induction of CYP1A1 in the mouse hepatoma cell line. PMID: 15535986
    30. Cyp1a1 protein may play a role in murine development and that regulation of the Cyp1a1 gene during this period is either through the action of a high affinity Ah receptor ligand or by an alternative regulatory pathway PMID: 15572371
    31. CYP1a1, a member of the CYP1 gene family, plays an important role in the metabolism of many xenobiotic compounds. PMID: 16169145
    32. We speculate that such mitochondrial CYPs may be involved in the generation, or mitigation, of the well-known TCDD-inducible oxidative stress response. PMID: 16516144
    33. mouse MT-CYP1A1 is an extrinsic membrane protein, which exhibited high FDX1 plus FDXR-mediated N-demethylation of a number of tricyclic antidepressants, pain killers, anti-psychotics, and narcotics that are poor substrates for microsomal CYP1A1 PMID: 16899466
    34. After a single dose of 0.1 mg/kg [(14)C]PhIP, lung PhIP-DNA adduct levels in Cyp1a1-null mice were significantly lower than in wild-type mice. PMID: 17052995
    35. NOS2 induction and NO production may be involved in TNF-alpha- and LPS-mediated downregulation of Cyp1a1 without affecting the downregulation of Nqo1. PMID: 17588258
    36. arylhydrocarbon receptor nuclear translocator-dependent pathway in gut has an important role in regulation of the metabolism of dietary CYP1A1 inducers and whole-body CYP1A1 expression PMID: 17607366
    37. HDAC1 plays a central role in Cyp1a1 expression PMID: 17707923
    38. Determined CYP1A1 expression after PBDE exposure and Coxsackievirus infection alone and in combination. PMID: 17964053
    39. Determined CYP1A1 expression/activity after PBDE exposure and Coxsackievirus infection alone and in combination. PMID: 17964055
    40. Overexpression of CYP1A1 shifts the balance of the metabolic activities in the skin of AhRR(-/-) mice in favor of the detoxification of carcinogens. PMID: 18022386
    41. Drug metabolizing enzyme induction of Cyp1a1 is reported in experimental fatty liver. PMID: 18488193
    42. broccoli-derived SUL can directly induce Cyp1a1 gene expression in an AhR-dependent manner and represents a novel mechanism by which sulforaphane induces this enzyme PMID: 19013013
    43. Cyp1a1 and Cyp1a2 do not play a dominant role in AHR-mediated vascular development. PMID: 19261855
    44. mouse liver CYP1A1-specific enzyme activities are between 38- and 170-fold higher than human CYP1A1-specific enzyme activities (per unit of mRNA), whereas mouse versus human CYP1A2 enzyme activities (per unit of mRNA) are within 2.5-fold of one another. PMID: 19285097
    45. Results indicate that rutaecarpine induces CYP1A1 expression through AhR- and calcium-dependent mechanisms. PMID: 19853001

    显示更多

    收起更多

  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Cytoplasm.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: mmu:13076

    STRING: 10090.ENSMUSP00000034865

    UniGene: Mm.14089