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  4. Recombinant Mouse Cystathionine gamma-lyase (Cth)

Recombinant Mouse Cystathionine gamma-lyase (Cth)

  • 货号：
    CSB-YP819743MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP819743MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP819743MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP819743MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP819743MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cth
  • Uniprot No.：
  • 别名：
    CthCystathionine gamma-lyase; EC 4.4.1.1; Cysteine-protein sulfhydrase; Gamma-cystathionase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MQKDASLSGF LPSFQHFATQ AIHVGQEPEQ WNSRAVVLPI SLATTFKQDF PGQSSGFEYS RSGNPTRNCL EKAVAALDGA KHSLAFASGL AATITITHLL KAGDEIICMD EVYGGTNRYF RRVASEFGLK ISFVDCSKTK LLEAAITPQT KLVWIETPTN PTLKLADIGA CAQIVHKRGD IILVVDNTFM SAYFQRPLAL GADICMCSAT KYMNGHSDVV MGLVSVNSDD LNSRLRFLQN SLGAVPSPFD CYLCCRGLKT LQVRMEKHFK NGMAVARFLE TNPRVEKVVY PGLPSHPQHE LAKRQCSGCP GMVSFYIKGA LQHAKAFLKN LKLFTLAESL GGYESLAELP AIMTHASVPE KDRATLGIND TLIRLSVGLE DEQDLLEDLD RALKAAHP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
  • 基因功能参考文献：
    1. Cystathionine gamma lyase-H2S was a dominant H2S generating system in osteoclasts, while cystathionine beta-synthase did not generate H2S. PMID: 29730290
    2. ZYZ-803 stimulated the expression of cystathionine gamma-lyase (CSE) for H2S generation and the activity of endothelial NO synthase (eNOS) for NO production.Blocking CSE and/or eNOS suppressed ZYZ-803-induced H2S and NO production and cardioprotection. PMID: 29288927
    3. Golgi stress response elicited by monensin stimulates CSE by acting via ATF4 with characteristics distinguishable from the endoplasmic reticulum stress response PMID: 29317536
    4. The results of this study show that deletion of the cystathionine-gamma-lyase gene has no effects on hypoxia-induced changes in TASK K(+) channel activity. PMID: 28851593
    5. CSE is critical for the progression of kidney fibrosis. PMID: 28842346
    6. renal expression of CTH and other H2S-producing enzymes was markedly suppressed after IRI, which could be an integrated adaptive response for renal cell protection PMID: 27273292
    7. This study suggests that the CSE/H2S system is involved in the pathogenesis of obesity in mice. PMID: 29191638
    8. Data show that CSE plays an important role as an inhibitor of inflammation in the liver by producing H2S from L-cysteine. PMID: 27848249
    9. Study shows maintaining CSE expression is critically important for stress adaptation during posttraumatic acute lung injury and CS-induced COPD, most likely in a gender-dependent manner. PMID: 27685807
    10. CSE-derived H2S contributes to mouse uterus homeostasis. Sildenafil reduces uterus contractility and its effect involves CSE-derived H2S. PMID: 27326921
    11. The antiatherosclerotic effect of estrogen is mediated by CSE-generated H2S PMID: 27986657
    12. Cystathionine-Gamma-Lyase Gene Deletion is associated with reduced inflammation and liver damage. PMID: 27518439
    13. this study reveals the molecular basis for the differential expression of Cth in mouse models of essential hypertension under basal and pathophysiological conditions. PMID: 27865915
    14. the findings of this study indicate that a deficiency in 3MST does not significantly affect endotoxemia, while a deficiency in CBS or CSE slightly ameliorates the outcome of LPS-induced endotoxemia in vivo. PMID: 27748832
    15. lung MPO activity increased following induction of sepsis with CLP while siRNA treatment significantly reduced MPO activity. Liver and lung cytokine and chemokine levels in CLP-induced sepsis reduced following treatment with siRNA. These findings show a crucial pro-inflammatory role for H2S synthesized by CSE in macrophages in sepsis and suggest CSE gene silencing with siRNA as a potential therapeutic approach for this co PMID: 26949091
    16. H2S augmented the S-sulfhydration of the rate-limiting gluconeogenic enzymes and PGC-1alpha and increased their activities, which were lower in untreated : To investigate the regulation of hepatic glucose production by cystathionine gamma-lyase KO hepatocytes PMID: 26401978
    17. Endogenous cystathionine gamma-lyase/Hydrogen sulfide regulates ischaemic vascular remodelling mediated during hind limb ischaemia through NO-dependent monocyte recruitment PMID: 26194202
    18. The results of this study suggest that CSE is not critically involved in chronic pain signaling in mice and that sources different from CSE mediate the pain relevant effects of H2S. PMID: 26271715
    19. CSE-H2S increased PPARgamma activity by direct sulfhydration at the C139 site, thereby changing glucose into triglyceride storage in adipocytes. PMID: 26946260
    20. Therefore, our data suggest that DNA hypermethylation of CpG rich region in cse promoter might contribute to the decrease of cse transcription and H2S production in macrophages, and thus contribute to atherosclerosis development. PMID: 26692478
    21. 3-Mercaptopyruvate sulphurtransferase and not cystathionine gamma-lyase is the primary regulator of coronary artery hydrogen sulfide production and function. PMID: 26519030
    22. These data confirm a key role for the H2S-generating enzymes Cbs and Cth in pulmonary vascular development and homeostasis and in lung alveolarization. PMID: 26232299
    23. GPBAR1 plays a role in secondary bile acid induced vasodilation via reglation of cystathionine gamma-lyase. The GPBAR1/CSE pathway might contribute to endothelial dysfunction and hyperdynamic circulation in liver cirrhosis. PMID: 25934094
    24. CTH inhibition did not affect the oviductal embryo transport, although delay of early embryo development was observed. PMID: 24914509
    25. CSE is present in adrenocortical cells and accounts for H2S generation in adrenal glands. CSE is critical for maintenance of mitochondrial function and glucocorticoid production in adrenal cortex. PMID: 24702258
    26. CSE and hydrogen sulfide modulate adipokine secretion in 3T3-L1 cells under high glucse exposure. PMID: 25277804
    27. Congenital deficiency or chemical inhibition of CSE increases thiosulfate levels in the liver and prevents ALF at least in part by augmentation of antioxidant and anti-apoptotic mechanisms. PMID: 23758073
    28. demonstration of a major depletion of cystathionine gamma-lyase (CSE), the biosynthetic enzyme for cysteine, in Huntington's disease tissues, which may mediate Huntington's disease pathophysiology PMID: 24670645
    29. LPS increased the expressions of cystathionine gamma-lyase and biosynthesis of Hydrogen sulfide... the effects of LPS were not present in TLR4(-/-) mice, indicating the crucial role of TLR4 in LPS-induced expression of CSE and biosynthesis of H2S PMID: 23877364
    30. Cystathionine gamma-lyase is a potent inducer of calcium resorption. It may act in early stages of osteoclastogenesis. Knockdown suppressed expression of osteoclast differentiation markers. PMID: 24357058
    31. Gene silencing the cystathionine gamma-lyase gene inhibited the proinflammatory cytokines and hydrogen sulfide production. PMID: 23838794
    32. These data indicate that endogenous CSE/H2 S system contributes to TNF-alpha-caused insulin resistance in 3T3-L1 adipocytes. PMID: 23080424
    33. These results indicate that endogenous hydrogen sulfide generated by CSE plays a key proinflammatory role via NF-kappaB activation in caerulein-induced pancreatitis. PMID: 24008358
    34. This study showed that CSE was the predominant H2S-generating enzyme, as it was found in 50% of the neurons and in presumptive glial cells in mice. PMID: 23904600
    35. Endogenous H2S is required for healthy placental vasculature, and a decrease in cystathionine gamma-lyase/H2S activity may contribute to the pathogenesis of preeclampsia. PMID: 23704251
    36. Cystathionine gamma-lyase protects against renal ischemia/reperfusion by modulating oxidative stress. PMID: 23449534
    37. In the setting of heart failure following transverse aortic constriction, CSE knockout mice exhibit significantly greater cardiac dilatation and dysfunction than wild-type mice. PMID: 23393010
    38. Differential effects of cystathionine-gamma-lyase-dependent vasodilatory H2S in periadventitial vasoregulation of rat and mouse aortas. PMID: 22870268
    39. Vitamin D up-regulates glucose transporter 4 (GLUT4) translocation and glucose utilization mediated by cystathionine-gamma-lyase (CSE) activation and H2S formation in 3T3L1 adipocytes. PMID: 23074218
    40. The 6 x Met-fed Cth(-/-) (not 1 x Met-fed Cth(-/-) or 6 x Met-fed wild type) mice displayed acute hepatitis, which was characterized by markedly elevated levels of serum alanine/aspartate aminotransferases and serum/hepatic lipid peroxidation PMID: 22387178
    41. overproduction of MG in CSE(-/-) mice is due to a H(2)S-mediated down-regulation of the PGC-1alpha-FBPase pathway, further suggesting the important role of H(2)S in the regulation of glucose metabolism and MG generation. PMID: 22216325
    42. CSE is a critical factor in GSH synthesis and may act to protect the liver and kidney from a variety of conditions that cause ER stress. PMID: 22215680
    43. the CSE/H(2)S system plays a critical role in regulating beta-cell functions PMID: 21718679
    44. The CSE is critical for cysteine biosynthesis through the transsulfuration pathway and the combination of CSE deficiency and lack of dietary cysteine supply would threaten life sustainability. PMID: 21310231
    45. The effects of CBS AND CSE gene transfer on the homocysteine-mediated inflmmation of mesangial cells induced by hydrogen sulfide and MCP-1 and MIP-2 induction are reported. PMID: 20943958
    46. Demonstrate an essential role of cystathionine gamma-lyase/H2S system in regulating vascular smooth muscle cell proliferation. PMID: 20051385
    47. The purpose of this study was to identify the seleno-L-methionine (L-SeMet) alpha,gamma-elimination enzyme that catalyzes L-SeMet to generate methylselenol (CH3SeH) PMID: 16327076
    48. Caerulein increased the level of cystathionine gamma-Lyase. PMID: 17488480
    49. Te CGL antiserum recognized the correct molecular weight protein for mouse retina. PMID: 17853447
    50. study shows H2S is physiologically generated by cystathionine gamma-lyase (CSE) & genetic deletion CSE markedly reduces H2S levels in serum & tissue; mice lacking CSE display pronounced hypertension & diminished endothelium-dependent vasorelaxation PMID: 18948540

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Trans-sulfuration enzymes family
  • 组织特异性：
    Detected in liver and kidney, and at lower levels in small intestine (at protein level). Highly expressed in liver and kidney. detected at lower levels in stomach, small intestine and adipose tissue.
  • 数据库链接：

    KEGG: mmu:107869

    STRING: 10090.ENSMUSP00000113672

    UniGene: Mm.28301