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  4. Recombinant Mouse Crumbs protein homolog 3 (Crb3), partial

Recombinant Mouse Crumbs protein homolog 3 (Crb3), partial

  • 中文名称：
    Recombinant Mouse Crumbs protein homolog 3(Crb3),partial,Yeast
  • 货号：
    CSB-YP854483MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Crumbs protein homolog 3(Crb3),partial,Yeast
  • 货号：
    CSB-EP854483MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Crumbs protein homolog 3(Crb3),partial,Yeast
  • 货号：
    CSB-EP854483MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Crumbs protein homolog 3(Crb3),partial,Yeast
  • 货号：
    CSB-BP854483MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Crumbs protein homolog 3(Crb3),partial,Yeast
  • 货号：
    CSB-MP854483MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Crb3
  • Uniprot No.：
  • 别名：
    Crb3Protein crumbs homolog 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions. Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7.
  • 基因功能参考文献：
    1. YAP promotes myelin and non-myelin genes transcription while the polarity protein Crb3, localized at the tips of the myelin sheath, activates the Hippo pathway to temper YAP activity, therefore allowing for optimal myelin growth. PMID: 27435623
    2. Crb3 knockdown in mammalian cells affects the dynamics of IFT particle movement. PMID: 28882989
    3. Crumbs3 sustains epithelial tissue morphogenesis and is essential to maintain apical membrane identity in kidney epithelial cells. PMID: 26631503
    4. Crb3, a Crumbs isoform that determines epithelial apical domain identity, is required for airway differentiation by controlling the localization of the transcriptional regulator Yap. PMID: 26235047
    5. The presence of Crb3 in many organs and the regularity of Crb3 distribution in the process of mouse embryonic development indicate that Crb3 protein plays an important role in establishing and maintaining the polarity of mouse embryos. PMID: 25131306
    6. Data indicate that Crb3 (Crumbs3) is crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying ezrin-containing cytoskeleton. PMID: 24164893
    7. the presence of CRB3 protein in the mouse retina PMID: 23226298

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  • 亚细胞定位：
    Apical cell membrane; Single-pass type I membrane protein. Cell junction, tight junction.
  • 组织特异性：
    Expressed in the apical renal tubules (at protein level). Expressed in the retinal pigment epithelium.
  • 数据库链接：

    KEGG: mmu:224912

    STRING: 10090.ENSMUSP00000071729

    UniGene: Mm.284447