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  4. Recombinant Mouse Crossover junction endonuclease MUS81 (Mus81)

Recombinant Mouse Crossover junction endonuclease MUS81 (Mus81)

  • 中文名称：
    小鼠Mus81重组蛋白
  • 货号：
    CSB-YP846013MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mus81重组蛋白
  • 货号：
    CSB-EP846013MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mus81重组蛋白
  • 货号：
    CSB-EP846013MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mus81重组蛋白
  • 货号：
    CSB-BP846013MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mus81重组蛋白
  • 货号：
    CSB-MP846013MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Mus81
  • Uniprot No.：
  • 别名：
    Mus81; Crossover junction endonuclease MUS81; EC 3.1.22.-
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-551
  • 氨基酸序列
    MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRSG KEAKILQHFG DRLCRMLDEK LKQHLASGGD HAPSSPSGKK GASKGPPEQV QDSSMPVPTQ PQAGSTSVGY WPAQNSGARE ILLQLYREHL NSDGHSFLTK EELLQKCAQK TPRVVPGSSK PWPALRSLLH RNLILGTHRP ARYALTPEGL ELAQKLAEAE GLSTRHAGFR PEEHHGEDSA VPEALSEPGT TEGAVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG LGHRVYLVEE HGSVHNLSLP ESTLLQAVTN TQVIDGFFVK RTMDIKESAG YLALLTKGLE RLYQGHTLRS RPWGAPGAAE SEAKPSTNPL CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AAVVDRYSTP ASLLAAYDAC ATAKEQEMLL STIKCGRLQR NLGPALSRTL YQLYCSHSPL S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
  • 基因功能参考文献：
    1. this study shows that immune rejection of prostate cancer cells relies on Mus81 and STING PMID: 27178469
    2. Our study highlights the importance of the cooperation between Rad54 and Mus81 for mediating DNA DSB repair and restraining chromosome missegregation. PMID: 26876210
    3. Mus81 knockdown improves the chemosensitivity of HCC cells by inducing S-phase arrest and promoting apoptosis through CHK1 pathway, suggesting Mus81 as a novel therapeutic target for HCC. PMID: 26714930
    4. Using mice deficient in both Mus81 and the FA pathway protein FancC, we show both proteins cooperate in parallel pathways, as concomitant loss of FancC and Mus81 triggered cell-type-specific proliferation arrest, apoptosis and DNA damage accumulation in utero. PMID: 25056314
    5. Data indicate that SLX1 and MUS81-EME1 nucleases act together to resolve Holliday junctions (HJs) in a manner that requires tethering to SLX4. PMID: 24076219
    6. FBH1 helicase activity is required to eliminate cells with excessive replication stress through the generation of MUS81-induced DNA double-strand breaks. PMID: 23361013
    7. data indicate that an important role for Chk2 is maintaining lymphocyte development and that dual inactivation of Chk2 and Mus81 remarkably inhibits cancer PMID: 21625617
    8. studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression PMID: 15205536
    9. Mus81 acts at a late step in the repair of cross-link-induced lesions PMID: 16107704
    10. Mus81-Eme1- and Rad54-mediated homologous recombination are involved in the same DNA replication-dependent interstrand crosslinks repair pathway PMID: 17036055
    11. Data suggest that Mus81 suppresses chromosomal instability by converting potentially detrimental replication-associated DNA structures into intermediates that are more amenable to DNA repair. PMID: 17934473
    12. Analysis of meiotic progression in Mus81-nullizygous mice, and our results implicate the MUS81 pathway as a regulator of crossover frequency and placement in mammals. PMID: 18787696
    13. Fndings support a role for Mus81 in the resolution of replication-associated DNA damage associated with this genotoxic agent, by converting Cr[VI]-DNA lesions into a form more amenable for homologous recombination. PMID: 19026666

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    XPF family
  • 组织特异性：
    Expressed highly in testis. Expressed also in bone marrow, brain, thymus and to a lesser extent in heart and skeletal muscle, colon, kidney and spleen.
  • 数据库链接：

    KEGG: mmu:71711

    STRING: 10090.ENSMUSP00000114895

    UniGene: Mm.27697