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  4. Recombinant Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 (Hsd11b2)

Recombinant Mouse Corticosteroid 11-beta-dehydrogenase isozyme 2 (Hsd11b2)

  • 中文名称：
    小鼠Hsd11b2重组蛋白
  • 货号：
    CSB-YP010765MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Hsd11b2重组蛋白
  • 货号：
    CSB-EP010765MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Hsd11b2重组蛋白
  • 货号：
    CSB-EP010765MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Hsd11b2重组蛋白
  • 货号：
    CSB-BP010765MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Hsd11b2重组蛋白
  • 货号：
    CSB-MP010765MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hsd11b2
  • Uniprot No.：
  • 别名：
    Hsd11b2; Hsd11k; Corticosteroid 11-beta-dehydrogenase isozyme 2; EC 1.1.1.-; 11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; NAD-dependent 11-beta-hydroxysteroid dehydrogenase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MERWPWPSGG AWLLVAARAL LQLLRSDLRL GRPLLAALAL LAALDWLCQR LLPPPAALVV LAGAGWIALS RLARPPRLPV ATRAVLITGC DTGFGKETAK KLDAMGFTVL ATVLDLNSPG ALELRDLCSP RLKLLQMDLT KAEDISRVLE ITKAHTASTG LWGLVNNAGL NIVVADVELS PVATFRKCME VNFFGALELT KGLLPLLRHS RGRIVTVGSP AGDMPYPCLA AYGTSKAAIA LLMDTFGCEL LPWGIKVSII KPGCFKTDAV TNVNLWEKRK QLLLANIPRE LLQAYGEDYI EHVHGQFLNS LRMALPDLSP VVDAIIDALL AAQPRSRYYP GRGLGLMYFI HHYLPEGLRR CFLQNFFINH LLPRALRPGQ HGPAPA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
  • 基因功能参考文献：
    1. Kidney-specific deficiency of 11beta-HSD2 leads to Salt-dependent Hypertension, which is attributed to mineralocorticoid receptor-epithelial sodium channel-Na(+)-Cl(-) cotransporter activation in the kidney. PMID: 28559392
    2. Rac1 GTPase regulates 11beta-HSD2 expression, mineralocorticoid receptor activation, and mineralocorticoid receptor-mediated pro-fibrotic signaling. PMID: 28320863
    3. Reduced brain 11betaHSD2 promotes a hunger for salt and salt sensitivity. 11betaHSD2-positive neurons integrate salt appetite and the blood pressure response to dietary sodium through a mineralocorticoid receptor-dependent pathway. PMID: 26951843
    4. 11betaHSD2 inhibition suppressed lung tumor growth and invasion in association with increased tissue active glucocorticoid levels, decreased COX-2 expression, inhibition of ERK and mTOR signaling pathways. PMID: 26011146
    5. placental 11bHSD2 expression is significantly reduced in pregnancies complicated by intrahepatic cholestasis of pregnancy. PMID: 24262137
    6. results show that intestinal epithelial 11ssHSD2 activity contributes to increased COX-2 expression in Apc+/min intestinal adenomas and that 11ssHSD2 deficiency in intestinal epithelial cells suppresses adenoma development and growth PMID: 23741059
    7. 11beta-hydroxysteroid dehydrogenase-2 is a cortisol-inactivating enzyme with a role in keratinocyte cell proliferation and basal cell proliferation PMID: 23362866
    8. Reduced 11beta-hydroxysteroid dehydrogenase type 2 causes salt sensitivity of blood pressure because of impaired renal natriuretic capacity. PMID: 22777941
    9. findings implicate DNA methylation as a mechanism by which prenatal stress alters HSD11B2 gene expression PMID: 22761903
    10. This study found that 11beta-hydroxysteroid dehydrogenase type 2 knockout mice develop nephrogenic diabetes insipidus. PMID: 22622456
    11. evidence for a weak or absent expression of neonatal renal 11betaHSD2 that is conserved among species PMID: 22359645
    12. HSD2 inhibition and increasing corticosterone adult physiological levels both can independently upregulate neural progenitor cell apoptosis in the perinatal period. PMID: 21530661
    13. 11beta-HSD2 plays no role in acute inflammatory responses PMID: 22067318
    14. Prenatal exposure to ethanol reduced expression of placental 11beta-HSD-2. PMID: 20627497
    15. Ligand-receptor interaction between triterpenoids and the 11beta-hydroxysteroid dehydrogenase type 2 (11betaHSD2) enzyme predicts their toxic effects against tumorigenic r/m HM-SFME-1 cells. PMID: 21880714
    16. Reduced activity of 11betaHSD2 causes salt sensitivity of blood pressure PMID: 21282561
    17. The activation of mineralocorticoid receptors in mice deficient in Apolipoprotein E and 11beta-hydroxysteroid dehydrogenase type 2 and subsequent athersclerosis are reported. PMID: 21106873
    18. Role of 11beta-hydroxysteroid dehydrogenase type 2 in aortic responses to glucocorticoids. PMID: 12925564
    19. 11beta-hydroxysteroid dehydrogenase type 2 acts as to protect the developing nervous system from the deleterious consequences of glucocorticoid overexposure PMID: 16289840
    20. Variable expression of HSD11B2 in vascular endothelial cells is reported. PMID: 18573267
    21. Restriction of fetal growth in 11beta-HSD2(-/-) mice is mediated, at least in part, via altered placental transport of nutrients and reduction in placental vascularization. PMID: 18845627
    22. Hedgehog signaling has a protective effect in glucocorticoid-induced mouse neonatal brain injury through an 11betaHSD2-dependent mechanism. PMID: 19164857
    23. Corticosterone regulation in the lung, specifically the reduction of active corticosterone by 11betaHSD2, may influence the progression of granuloma formation in response to the mycobacterial glycolipid. PMID: 19246936

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  • 亚细胞定位：
    Microsome. Endoplasmic reticulum.
  • 蛋白家族：
    Short-chain dehydrogenases/reductases (SDR) family
  • 组织特异性：
    Highly expressed in kidney. Also found in colon and small intestine. Not expressed in the adrenal gland.
  • 数据库链接：

    KEGG: mmu:15484

    STRING: 10090.ENSMUSP00000034363

    UniGene: Mm.5079