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  4. Recombinant Mouse Choline kinase alpha (Chka)

Recombinant Mouse Choline kinase alpha (Chka)

  • 中文名称：
    Recombinant Mouse Choline kinase alpha(Chka),Yeast
  • 货号：
    CSB-YP005353MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Choline kinase alpha(Chka),Yeast
  • 货号：
    CSB-EP005353MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Choline kinase alpha(Chka),Yeast
  • 货号：
    CSB-EP005353MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Choline kinase alpha(Chka),Yeast
  • 货号：
    CSB-BP005353MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Choline kinase alpha(Chka),Yeast
  • 货号：
    CSB-MP005353MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Chka
  • Uniprot No.：
  • 别名：
    Chka; ChkCholine kinase alpha; CK; EC 2.7.1.32; CHETK-alpha; Ethanolamine kinase; EK; EC 2.7.1.82
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-453
  • 氨基酸序列
    MKTKFCTGGE AEPSPLGLLL SCGGNAAPTP GVGQQRDAAG ELESKQLGGR TQPLALPPPP PPPLPLPPPP SPPLADEQPE PRTRRRAYLW CKEFLPGAWR GLREDQFHIS VIRGGLSNML FQCSLPDSIA SVGDEPRKVL LRLYGAILKM RSCNKEGSEQ AQNENEFQGA EAMVLESVMF AILAERSLGP KLFGIFPQGR LEQFIPSRRL DTEELRLPDI SAEIAEKMAT FHGMKMPFNK EPKWLFGTME KYLNQVLRLK FSREARVQQL HKILSYNLPL ELENLRSLLQ YTRSPVVFCH NDCQEGNILL LEGQENSERR KLMLIDFEYS SYNYRGFDIG NHFCEWMYDY TYEKYPFFRA NIQKYPSRKQ QLHFISSYLT TFQNDFESLS SEEQFATKED MLLEVNRFAL ASHFLWGLWS IVQAKISSIE FGYMEYAQAR FEAYFDQKRK LGV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.
  • 基因功能参考文献：
    1. Enforced expression of either CK(alpha) or CCTalpha increased the rate of synthesis and the amount of PtdCho, and these cells initiated neuronal differentiation. PMID: 20525991
    2. Lack of muscle degeneration in forelimbs of Chkb(-/-) mice is due to abundant choline kinase alpha and the stable homeostasis of phosphatidylcholine. PMID: 20026284
    3. Full-length cDNAs or their truncated or alanine-mutated versions for mouse CK-alpha1 and CK-beta were expressed in COS-7 cells. PMID: 16490392
    4. ChoK may have a role in carcinogenesis PMID: 16820874
    5. Data suggest that the choline kinase alpha promoter has a distal element (-875/-867) that binds c-jun and the binding of c-jun is enhanced by treatment with CCl4. PMID: 17728180
    6. Chka is an essential gene for early embryonic development, but adult mice do not require full expression of the gene for normal levels of phosphatidylcholine. PMID: 18029352

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Choline/ethanolamine kinase family
  • 组织特异性：
    Expressed ubiquitously with the highest level in testis.
  • 数据库链接：

    KEGG: mmu:12660

    STRING: 10090.ENSMUSP00000071933

    UniGene: Mm.225505