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  4. Recombinant Mouse Cell death activator CIDE-3 (Cidec)

Recombinant Mouse Cell death activator CIDE-3 (Cidec)

  • 中文名称：
    Recombinant Mouse Cell death activator CIDE-3(Cidec),Yeast
  • 货号：
    CSB-YP005433MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Cell death activator CIDE-3(Cidec),Yeast
  • 货号：
    CSB-EP005433MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Cell death activator CIDE-3(Cidec),Yeast
  • 货号：
    CSB-EP005433MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Cell death activator CIDE-3(Cidec),Yeast
  • 货号：
    CSB-BP005433MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Cell death activator CIDE-3(Cidec),Yeast
  • 货号：
    CSB-MP005433MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cidec
  • Uniprot No.：
  • 别名：
    Cidec; Fsp27Cell death activator CIDE-3; Cell death-inducing DFFA-like effector protein C; Fat-specific protein FSP27
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-239
  • 氨基酸序列
    MDYAMKSLSL LYPRSLSRHV AVSTAVVTQQ LVSKPSRETP RARPCRVSTA DRKVRKGIMA HSLEDLLNKV QDILKLKDKP FSLVLEEDGT IVETEEYFQA LAKDTMFMVL LKGQKWKPPS EQRKKRAQLA LSQKPTKKID VARVTFDLYK LNPQDFIGCL NVKATLYDTY SLSYDLHCYK AKRIVKEMLR WTLFSMQATG HMLLGTSSYM QQFLDATEEE QPAKAKPSSL LPACLKMLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.
  • 基因功能参考文献：
    1. the role of gp78 and cidec in hepatic steatosis, were investigated. PMID: 28656280
    2. The current study demonstrated that insulin represses fasting-induced Fsp27 expression in the liver. The Fsp27 decreased by insulin is likely to cause the lower fat accumulation in liver. PMID: 28566630
    3. Fatty acids prevent CIDEC deacetylation by promoting the dissociation of CIDEC from HDAC6, resultin in increased association of CIDEC with PCAF on the endoplasmic reticulum. PMID: 28287402
    4. We show that partial silencing Fsp27 in either model results in the robust decrease in visceral fat, improved insulin sensitivity and whole-body glycemic control, and tissue-specific changes in transcripts controlling lipid oxidation and synthesis PMID: 27884961
    5. Lipid droplet (LD) expansion depends on the FSP27 carboxy-terminal domain (amino acids 131-239). The negative charge of acidic residues D215, E218, E219 and E220 in the polar carboxy-terminal region (amino acids 202-239) is essential for LD enlargement. PMID: 26921608
    6. Hepatic expression of FSP27/CIDEC is highly up-regulated in mice following chronic-plus-binge ethanol feeding and this up-regulation contributes to alcohol-induced liver damage. PMID: 26099526
    7. Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice. PMID: 25565658
    8. CREBH-Fsp27beta axis is important for regulating lipid droplet dynamics and triglyceride storage in the liver PMID: 25125366
    9. Adipocyte-specific disruption of fat-specific protein 27 causes hepatosteatosis and insulin resistance in high-fat diet-fed mice. PMID: 25477509
    10. Promotion of FSP27 degradation may be an important factor responsible for the beneficial effect of AMPK activators on energy metabolism PMID: 25315694
    11. results demonstrate a crucial role for FSP27-ATGL interactions in regulating lipolysis, triglyceride accumulation, and insulin signaling in human adipocytes PMID: 24627478
    12. FSP27 mRNA expression in the liver depends on the etiology of fatty liver. PMID: 24189421
    13. Fsp27/CIDEC is a CREB target gene induced during early fasting in liver and regulated by FA oxidation rate. PMID: 23220584
    14. Perilipin1 (Plin1), another adipocyte-specific lipid droplet-associated protein, is an Fsp27 activator. PMID: 23481402
    15. FSP27 acts to constitutively limit the lipid droplet presence of ATGL, perilipin 1 plays an essential role in mediating the response of ATGL action to beta-adrenergic hormones. PMID: 23204327
    16. the high-yield expression of the mouse Fsp27 CIDE-N domain in Escherichia coli; a two-step purification protocol with high efficiency was established and crystallographic analysis was performed. PMID: 23192040
    17. a lipid droplet (LD)-binding protein that promotes lipid accumulation in adipocytes.(review) PMID: 22790028
    18. The differences in fat fractions in white adipose tissue (WAT) and brown adipose tissue(BAT) measured by MR methods in different mouse models were related to the different regulation effects of ob, seipin, or Fsp27 gene on developing WAT and BAT. PMID: 23149622
    19. A novel molecular mechanism of lipid droplet growth mediated by Fsp27 was uncovered. PMID: 22144693
    20. MKP-1 plays an important role in MAPK-mediated control of hepatic lipid homeostasis PMID: 21521693
    21. Findings demonstrate that overexpression of PeriA in white adipocytes reduces lipid droplet size by decreasing FSP27 expression and thereby inducing a brown adipose tissue-like phenotype. PMID: 21103377
    22. FSP27 acts as a molecular determinant that controls gene expression for a diversity of metabolic and signaling pathways and, in particular, the expression of regulatory factors. PMID: 20649970
    23. Fat-specific protein 27 undergoes ubiquitin-dependent degradation regulated by triacylglycerol synthesis and lipid droplet formation PMID: 20089860
    24. FSP27/Cidec as a novel adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation. PMID: 17884815
    25. roles for FSP27 in cell death and in adipocyte function PMID: 18198355
    26. FSP27 binds to lipid droplets and regulates their enlargement PMID: 18334488
    27. FSP27 is a direct mediator of PPARgamma-dependent hepatic steatosis. PMID: 18396136
    28. Fsp27 contributes to efficient energy storage in white adipocytes by promoting the formation of unilocular lipid droplets. PMID: 18654663
    29. Fsp27 acts as a novel regulator in vivo to control white adipose tissue identity, mitochondrial activity and insulin sensitivity. PMID: 18682832
    30. CIDEC is a regulator of adipocyte lipid metabolism and may be important for the adipocyte to adapt to changes in energy availability. PMID: 18702959
    31. These results suggest that PPARgamma2 is required for the transcriptional activity of Cidec during adipogenesis. PMID: 18845124

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  • 亚细胞定位：
    Lipid droplet. Endoplasmic reticulum. Nucleus. Note=Diffuses quickly on lipid droplet surface, but becomes trapped and clustered at lipid droplet contact sites, thereby enabling its rapid enrichment at lipid droplet contact sites.
  • 组织特异性：
    Expressed almost exclusively in adipose tissue, including subcutaneous and epididymal white adipose tissue (at protein level). Although abundantly present in brown adipose tissue at the mRNA level, the protein is almost undetectable in this tissue, or at
  • 数据库链接：

    KEGG: mmu:14311

    STRING: 10090.ENSMUSP00000032416

    UniGene: Mm.10026