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  4. Recombinant Mouse Cardiac phospholamban (Pln), partial

Recombinant Mouse Cardiac phospholamban (Pln), partial

  • 中文名称：
    小鼠Pln重组蛋白
  • 货号：
    CSB-YP018198MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pln重组蛋白
  • 货号：
    CSB-EP018198MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pln重组蛋白
  • 货号：
    CSB-EP018198MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pln重组蛋白
  • 货号：
    CSB-BP018198MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pln重组蛋白
  • 货号：
    CSB-MP018198MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pln; Cardiac phospholamban; PLB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 基因功能参考文献：
    1. Oxidative stress upregulates pVHL expression to induce PLN degradation in failing hearts. PMID: 29068413
    2. A single-dose injection of PLN-targeting locked nucleic acid antisense oligonucleotide improved contractility in pressure overload-induced cardiac dysfunction. PMID: 27811197
    3. PLN overexpression is associated with severe muscle atrophy and weakness. PMID: 28278204
    4. These data suggest that PLN is, at least partially, oligo-ubiquitinated at Lys(3) and degraded through Ser(16)-phosphorylation-mediated poly-ubiquitination during heart failure. PMID: 26966065
    5. the commercially available overexpressing phospholamban mouse phenotypically resembles human Centronuclear myopathy and could be used as a model to test potential mechanisms and therapeutic strategies. PMID: 26035394
    6. Cardioprotective effects of H2S are mediated through acGMP/PKG/phospholamban pathway. PMID: 25870184
    7. PLN pentamers reduce phosphorylation of monomers at baseline and delay monomer phosphorylation upon PKA stimulation leading to increased interaction of PLN monomers with SERCA2a. PMID: 25562800
    8. combined deletion of Phd2 and Phd3 dramatically decreased expression of phospholamban (PLN), resulted in sustained activation of calcium/calmodulin-activated kinase II (CaMKII), and sensitized mice to chronic beta-adrenergic stress-induced myocardial injury PMID: 26075818
    9. the N termini of SLN and PLB influence their respective unique functions PMID: 25882845
    10. CaMKII-dependent increase in PLN phosphorylation during reperfusion opposes rather than contributes to ischemia/reperfusion damage. PMID: 24949568
    11. TNAP plays a role in governing the phosphorylation status of phospholamban in the sarcoplasmic reticulum. PMID: 25015959
    12. SLN and PLN are co-expressed in most fibers, which suggests that super-inhibition of SERCAs may be physiologically important in the regulation of intracellular Ca2+ in human skeletal muscle. PMID: 24358354
    13. Epac1 KO exhibited decreased cardiac contractility with reduced phospholamban (PLN) phosphorylation at serine-16, the major PKA-mediated phosphorylation site. PMID: 24892712
    14. Phospholamban knockout breaks cell-wide propagating spontaneous Ca2+ waves in isolated ventricular myocytes & protects RyR2-mutant mice from stress-induced ventricular tachycardia. PMID: 23856523
    15. Data indicate that phospholamban (PLB) is not involved in heat generation and that sarcolipin (SLN) alone is responsible for muscle thermogenesis. PMID: 23341466
    16. PLN is a critical target for CaMKII effects on FFR, while CaMKII effects on frequency-dependent acceleration of relaxation partially require PLN-alternative targets. PMID: 22796260
    17. Dynamic regulation of the SERCA pump by phospholamban and/or sarcolipin maintains cardiac contractility in normal conditions and during pathophysiological states. PMID: 20833651
    18. Data indicate that cardiomyocytes from PLN-KO/CaMKII-TG mice showed poor viability, improved by inhibiting SR Ca(2+) release and mitochondrial Ca(2+) loading. PMID: 19959778
    19. although both phospholamban phosphorylation sites are involved in the mechanical recovery after myocardial ischemia, Thr17 appears to play a major role. PMID: 12763747
    20. PLB phosphorylation by CaMKII plays an important role in limiting the decline in Ca transients (and contraction) during acidosis PMID: 14734049
    21. Enhanced contractile heart function is enriched in PLN-knockout mutant hearts PMID: 14982994
    22. increased PLN expression in slow-twitch skeletal muscle is associated with impaired contractile function and muscle remodeling PMID: 15059971
    23. Results show that caffeine-induced CaMKII activation and phospholamban phosphorylation play a role in the relaxation of gastric fundus smooth muscles. PMID: 15659716
    24. The expression of SLN and PLB mRNA and protein relative to SERCA1 or SERCA2 was assessed in ventricle, atrium, and skeltal msucle of mouse, rat, rabbit and pig. PMID: 15801907
    25. the nonreversible superinhibitory function of mutant PLN-R14Del may lead to inherited dilated cardiomyopathy and premature death in both humans and mice PMID: 16432188
    26. A 60% reduction of PLN transcript & protein occurred with persistent ss2AR activation, due to receptor overexpression in airway smooth muscle cells. This decreased the maximal airway contraction & the sensitivity to the agonist methacholine. PMID: 16849635
    27. There is a trend for increased Iso-induced apoptosis in AC3-I mice lacking PLN. Findings show CaMKII is proapoptotic in vivo and regulation of SR Ca(2+) content by PLN contributes to the antiapoptotic mechanism of CaMKII inhibition PMID: 16861697
    28. The observed trafficking of PLB to the plasma membrane suggests an important role for PLB during muscle differentiation, which is distinct from its previously recognized role in the regulation of the Ca(2+)-ATPase. PMID: 17287364
    29. cGMP dependent phosphorylation analysis showed that some proteins were phosphorylated by cGMP to a lesser extent in PLB-KO compared with WT myocytes, suggesting impaired cGMP-kinase function in PLB-KO cardiac myocytes. PMID: 17595525
    30. Aerobic interval training enhances cardiomyocyte contractility and Ca2+ cycling by phosphorylation of CaMKII and thr-17 of Pln. PMID: 17689560
    31. These findings suggest that PLB is an important modulator of gastric antrum smooth muscle contractility by modulation of SR Ca(2+) release and CaM kinase II activity. PMID: 18045856
    32. proteomics surveys of cardiac ventricle isolated from a mouse model of cardiomyopathy overexpressing a phospholamban mutant, was performed. PMID: 18056057
    33. Down-regulation of PLN led to enhanced cell shortening and relaxation and to a decrease in the time constant of calcium decay, signs of improved contractility and calcium handling. PMID: 18064719
    34. Suggest that in isolated mouse ventricular cardiac myocytes, the combined chronic CaMKII inhibition and PLN ablation slowed Ca(2+) release at physiological frequencies. PMID: 18359893
    35. Examine roles of NCX1 and Atp1a1 in phospholamban regulation of cardiomyocyte contractility. PMID: 18708446
    36. These data demonstrate that alphaKAP exhibits a novel interaction with SERCA2a and may serve to spatially position CaMKII isoforms at the SR and to uniquely modulate the phosphorylation of PLN. PMID: 19671701
    37. Data show that Akt interacts with and phosphorylates phospholamban at Thr(17) while silencing Akt signaling through the knock-out of phosphatidylinositol-dependent kinase-1 results in reduced phosphorylation of PLN. PMID: 19696029

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Phospholamban family
  • 数据库链接：

    KEGG: mmu:18821

    STRING: 10090.ENSMUSP00000045709

    UniGene: Mm.34145