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  4. Recombinant Mouse Calsenilin (Kcnip3)

Recombinant Mouse Calsenilin (Kcnip3)

  • 中文名称：
    小鼠Kcnip3重组蛋白
  • 货号：
    CSB-YP874112MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Kcnip3重组蛋白
  • 货号：
    CSB-EP874112MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Kcnip3重组蛋白
  • 货号：
    CSB-EP874112MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Kcnip3重组蛋白
  • 货号：
    CSB-BP874112MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Kcnip3重组蛋白
  • 货号：
    CSB-MP874112MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Kcnip3
  • Uniprot No.：
  • 别名：
    Kcnip3; Csen; Dream; Kchip3; Calsenilin; A-type potassium channel modulatory protein 3; DRE-antagonist modulator; DREAM; Kv channel-interacting protein 3; KChIP3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-256
  • 氨基酸序列
    MQRTKEAVKA SDGNLLGDPG RIPLSKRESI KWQRPRFTRQ ALMRCCLIKW ILSSAAPQGS DSSDSELELS TVRHQPEGLD QLQAQTKFTK KELQSLYRGF KNECPTGLVD EDTFKLIYSQ FFPQGDATTY AHFLFNAFDA DGNGAIHFED FVVGLSILLR GTVHEKLKWA FNLYDINKDG CITKEEMLAI MKSIYDMMGR HTYPILREDA PLEHVERFFQ KMDRNQDGVV TIDEFLETCQ KDENIMNSMQ LFENVI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.; Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.; May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of amyloid-beta formation.
  • 基因功能参考文献：
    1. DREAM inhibition markedly improves ATF6 processing in the hippocampus and that it might contribute to a delay in memory decline in Huntington mice. PMID: 29523177
    2. Genome-wide analysis of trigeminal neurons in daDREAM transgenic mice identified cathepsin L and the monoglyceride lipase as two new DREAM transcriptional targets related to pain. PMID: 26896746
    3. Our findings demonstrate for the first time that DREAM plays an important role in thrombosis and hemostasis in mice. PMID: 27903531
    4. association of PS1 carboxyl peptide (residues 445-467, HL9) with DREAM is calcium dependent and stabilized by a cluster of three aromatic residues: F462 and F465 from PS1 and F252 from DREAM. PMID: 27009418
    5. DREAM plays an important role in structural plasticity in the hippocampus. PMID: 26928278
    6. Terbium(III) binding preserves the folding state of DREAM with minimal changes to overall structure of DREAM. PMID: 26901070
    7. Results presented in this study show for the first time that down-regulation of DREAM promotes the degeneration of RGCs, amacrine cells, and bipolar cells. PMID: 26020793
    8. The association of calcium-bound calmodulin (CaM) with DREAM is mediated by a short amphipathic amino acid sequence located between residues 29 and 44 on DREAM. PMID: 26108881
    9. The protein DREAM decreases development of L-DOPA-induced dyskinesia in mice and reduces L-DOPA-induced expression of FosB, phosphoacetylated histone H3, and dynorphin-B in the striatum PMID: 24857398
    10. NS5806 directly interacts with KChIP3 and modulates the interactions between this calcium-binding protein and the T1 domain of the Kv4.3 channels through reorientation of helix 10 on KChIP3 PMID: 25228688
    11. These results provide the first evidence that DR/C/K3 plays a timing-dependent role in estradiol regulation of learning, memory, and plasticity. PMID: 24248121
    12. DREAM is a major master switch transcription factor. PMID: 24366545
    13. GCM1-directed villous trophoblast differentiation is repressed by DREAM PMID: 23300953
    14. Expression of DREAM in neuroblastoma cells enhances cisplatin mediated caspase-3 activity. PMID: 23524266
    15. Restoring CANT1 levels in neuroblastoma clones recovered the phenotype, thus confirming a key role of CANT1, and of the regulation of its gene by DREAM, in the control of protein synthesis and degradation PMID: 22451650
    16. In cardiomyocytes, [Ca2+]i-activated calmodulin kinase II (CaMKII) activates downstreamregulatory element (DRE) binding transcription factor DREAM, which consequently suppresses the expression of L-type calcium channel 1C-subunit gene (Cacna1c). PMID: 21486818
    17. Sumoylation regulates the nuclear localization of DREAM in differentiated neurons. PMID: 21070824
    18. endogenous BDNF is involved in spinal sensitization following inflammation and blockade of BDNF induction in DREAM transgenic mice underlies the failure to develop spinal sensitization PMID: 21167062
    19. Results demonstrate that DREAM plays a novel role in postsynaptic modulation of the NMDA receptor, and contributes to synaptic plasticity and behavioral memory. PMID: 20205763
    20. Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. PMID: 21059893
    21. Calsenilin and presenilin 1 can interact at physiologic levels; calsenilin is a developmentally regulated protein that is expressed primarily in the cerebellum and hippocampus. PMID: 12207970
    22. Results demonstrate that DREAM (DRE-antagonizing modulator) is a neural activity-stimulated late gene and suggest its involvement in adaptation to long-lasting neuronal activity. PMID: 12531529
    23. Calsenilin is markedly decreased not only in the damaged cortex and CA3 region of hippocampus at 24 h after kainic acid-induced seizure but also in a cell-culture model of seizure-like activity. PMID: 12648752
    24. calsenilin can be phosphorylated by casein kinase I and that its phosphorylation can be regulated by intracellular calcium PMID: 12837631
    25. Abeta peptide levels are reduced in calsenilin knock-out mice, demonstrating that calsenilin affects presenilin-dependent gamma-cleavage in vivo. Long-term potentiation in the dentate gyrus of hippocampus is enhanced in calsenilin knock-out mice PMID: 14534243
    26. KChIP3 transcripts were detected primarily in the layer V and deep layer VI of the cerebral cortex, the hippocampus, and the entire cerebellum. PMID: 15363885
    27. Results demonstrate that prodynorphin transcription is regulated by DREAM in a calcium-dependent manner. PMID: 16621893
    28. calsenilin and CtBP2 are present in synaptic vesicles and can interact in vivo PMID: 16787403
    29. Expression of DREAM in various cell types coupled with the universal role of calcium raise the possibility that these factors may play similar role in other secretory cells. PMID: 17241740
    30. A dense plexus of calsenilin-positive dendrites makes several basal contacts at cone pedicles, individual calsenilin-positive bipolar cell contacts five to seven cones, some calsenilin-positive dendrites also contact rod photoreceptors PMID: 18095322
    31. Ca2+-induced dimerization of DREAM may partially block the putative DNA-binding site. PMID: 18201103
    32. These results identify the PACAP-cAMP-Ca(2+)-DREAM cascade as a new pathway to activate GFAP gene expression during astrocyte differentiation. PMID: 18579744
    33. Lack of DREAM protein enhances learning and memory and slows brain aging. PMID: 19110430
    34. Male KChIP3 knockout (KO) mice showed significantly enhanced memory 24 hours after training and this study suggest a role for regulation of gene expression by KChIP3/DREAM/calsenilin in consolidation of contextual fear conditioning memories. PMID: 19223600
    35. DREAM (downstream regulatory element antagonist modulator) modulates TSHR activity through a direct protein-protein interaction that promotes coupling between the receptor and Galphas. PMID: 19299442

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Lipid-anchor. Endoplasmic reticulum. Golgi apparatus. Nucleus.
  • 蛋白家族：
    Recoverin family
  • 组织特异性：
    Highly expressed in brain. Isoform 1 or isoform 4 (T+ forms) are expressed at equal levels with isoform 2 or isoform 3 (T- forms). Primarily detected in the layer V and deep layer VI of the cerebral cortex, the hippocampus, and the entire cerebellum. Expr
  • 数据库链接：

    KEGG: mmu:56461

    STRING: 10090.ENSMUSP00000099504

    UniGene: Mm.315292