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  4. Recombinant Mouse Calpastatin,isoform 5 (Cast)

Recombinant Mouse Calpastatin,isoform 5 (Cast)

  • 中文名称：
    Recombinant Mouse Calpastatin,isoform 5(Cast),Yeast
  • 货号：
    CSB-YP004561MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Calpastatin,isoform 5(Cast),Yeast
  • 货号：
    CSB-EP004561MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Calpastatin,isoform 5(Cast),Yeast
  • 货号：
    CSB-EP004561MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Calpastatin,isoform 5(Cast),Yeast
  • 货号：
    CSB-BP004561MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Calpastatin,isoform 5(Cast),Yeast
  • 货号：
    CSB-MP004561MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cast
  • Uniprot No.：
  • 别名：
    Cast; Calpastatin; Calpain inhibitor
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-788
  • 氨基酸序列
    MSQPGPKPAA SPRPSRGAAA RHTQEHVNEK NIGSSSKPGE KKGSDEKKAA SLGSSQPSRP HVGEAATATK VTASSAATSK SPSMSTTETK AIPVNKQLEG PDQKRPREQA VKTESKKPQS SEQPVVHEKK SKGGPKEGSE PKNLPKHTSS TGSKHAHKEK ALSRSNEQMV SEKPSESKTK FQDVPSAGGE SVAGGGTVAT ALDKVVGKKK EQKPFTPASP VQSTPSKPSD KSGMDAALDD LIDTLGGHED TNRDDPPYTG PVVLDPMYST YLEALGIKEG TIPPEYRKLL EKNEGITQPL PDSPKPMGTD QAIDALSSDF TCSSPTGKQS EKEKSTGEIF KAQSAGVTRS SVPPKEKKRK VEEEVINDQA LQALSDSLGT RQPDPPSHVS QAEQVKEAKA KEERQEKCGE DEDTVPAEYR LKPAKDKDGK PLLPEPEETS KSLSESELIG ELSADFDRST YQDKPSTPAE KKSNDTSQTP PGETVPRASM CSIRSAPPKL ASLKGVVPED AVETLAGSLG TREADPEHEK TVEDKVKEKA KEEEHEKLGE KEETVPPDYR LEEVKDKDGK PLLPKESQEQ LAPLSDDFLL DALSQDFSSP ANISSLEFED AKLSAAISEV VSQTPAPSTH AAAPLPGTEQ KDKELDDALD ELSDSLGQRP PDPDENKPLD DKVKEKIKPE HSEKLGERDD TIPPEYRHLL DNDGKDKPEK PPTKKTEKPD QDRDPIDALS EDLDSCPSTT ETSKNTAKGK SKKTSSSKAS KDGEKTKDSS KKTEEVSKPK AKEDARHS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
  • 基因功能参考文献：
    1. The knockdown of CAST in mutant huntingtin-expressing cells and the knockout of the Cast gene in a knock-in mouse model of HD resulted in a worsening of the molecular HD-related hallmarks including elevated formation of huntingtin fragments and increased amount of respective aggregates PMID: 29355642
    2. Defective scar formation after myocardial infarction in calpastatin transgenic mice was associated with blunted CD4(+) T-cell and M2-macrophage recruitment. PMID: 26453333
    3. EC-specific transgenic CAST downregulated JAK/STAT signals, upregulated SOCS3, and depleted VEGF-C, counteracting unstable pathological neovessels and disease progression in tumors and oxygen-induced retinopathy lesions in mice. PMID: 25648699
    4. We observed that ischemia induction results in increased expression of CaN, Calp and Calpn. PMID: 24333421
    5. Over-expression of calpastatin enhances doxorubicin-induced cardiac injuries through calpain inhibition. PMID: 23455548
    6. Data show that genetic deficiency of calpastatin (CS) augmented Abeta amyloidosis, tau phosphorylation, microgliosis, and somatodendritic dystrophy, and increased mortality in amyloid precursor protein (APP) transgenic mice. PMID: 22173972
    7. PIN1 apparently restrains the ability of calpastatin to inhibit calpain, maintaining calpain activity in endothelial cells. PMID: 21982763
    8. Calpastatin is likely to be more closely associated with affective rather than cognitive aspects of brain function. PMID: 18803809
    9. Blocking endogenous calpain activity partially impairs insulin release in vivo and in vitro by targeting distal components of the insulin exocytotic machinery. PMID: 21099278
    10. Calpain inhibition by overexpressing CAST in transgenic mice attenuates skin allograft rejection by a delay in T-cell mobilization. PMID: 21268016
    11. Ectopic expression in pachytene spermatocytes suppressed germ cell apoptosis. PMID: 15223839
    12. apoptotic responses were augmented by calpastatin deficiency. Consistently calpastatin overexpression suppressed them PMID: 15691848
    13. calpain and caspase activation have roles in neurodegeneration, as demonstrated in calpastatin-transgenic mice PMID: 15699033
    14. Western blot analysis of the mouse brain showed reduced levels of calpastatin and rabaptin-5 and higher amount of neuronatin in PKU compared to the wild type. PMID: 15863237
    15. Findings suggest that calpastatin transgenic mice results in impairment of a step in the GLUT4 translocation process downstream of the insulin- and contraction-signaling pathways. PMID: 16705056
    16. Calpastatin is an important factor in the regulation of calpain-induced protein degradation in the brains of the affected mice, implying a role for calpastatin in attenuating AD pathology PMID: 17513017
    17. Calpastatin gene transfer prevented cardiovascular remodeling in AngII-dependent hypertension. PMID: 18258859

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  • 蛋白家族：
    Protease inhibitor I27 (calpastatin) family
  • 组织特异性：
    Isoform 2 is the major form in all tissues examined. Isoform 1 accounts for 5-10% in tissues such as skeletal muscle, liver and brain, and 30% in myoblasts. Isoforms 4 and 5 are testis-specific. Isoform 6 is highly expressed in heart and skeletal muscle w
  • 数据库链接：

    KEGG: mmu:12380

    STRING: 10090.ENSMUSP00000065275

    UniGene: Mm.29163