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  4. Recombinant Mouse CST complex subunit STN1 (Obfc1)

Recombinant Mouse CST complex subunit STN1 (Obfc1)

  • 货号：
    CSB-YP814312MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP814312MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP814312MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP814312MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP814312MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Stn1
  • Uniprot No.：
  • 别名：
    Stn1; Obfc1; CST complex subunit STN1; Alpha-accessory factor of 44 kDa; AAF-44; AAF44; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-378
  • 氨基酸序列
    PQPCLLMEC ESSPREEEIP PLFWGLDPVF LAFAKLYIKD ILEMKESQQV PGTYFYNGHP IRRVDIMGAV ISVKERETFY SYGVDDATGV INCVCWKKLS NAESSSDPAI LSTARELSMT SQLKKLQETI EQKTRIGIGD IIRVRGSVRM FREEREICAN IYYKVDDPVW NMQIARMLEL PKLYQKVYDQ PFRNPALQEE EALNNKDNLD LAGLTSLLSE KIKEFLQEKK MQSFYQQELE TVESLQSLAS RPVTHSTGSD QVELKDSGTS GVAQRVFKNA LQLLQEKGLV FQRDSGSDKL YYVTTKDKDL QQKIYHIIKE DCQKPNHMEK GCHLLHILNC VHLNLRWDLS KAVLQRVLEL LEDQSDIVST ADHYYAAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation. However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication.
  • 基因功能参考文献：
    1. OBFC1 is identical to the previously described 44 kDa subunit of DNA-pol-alpha/primase associated factor (AAF) which increases polymerase-alpha/primase template affinity and stimulate both DNA primase and polymerase-alpha activities in vitro. PMID: 19119139
  • 亚细胞定位：
    Nucleus. Chromosome, telomere.
  • 蛋白家族：
    STN1 family
  • 数据库链接：

    KEGG: mmu:108689

    STRING: 10090.ENSMUSP00000040944

    UniGene: Mm.218048