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  4. Recombinant Mouse Apolipoprotein A-IV (Apoa4)

Recombinant Mouse Apolipoprotein A-IV (Apoa4)

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  • 中文名称：
    Recombinant Mouse Apolipoprotein A-IV(Apoa4)
  • 货号：
    CSB-YP001916MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    Apoa4
  • Uniprot No.：
  • 别名：
    Apoa4; Apolipoprotein A-IV; Apo-AIV; ApoA-IV; Apolipoprotein A4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    45.0kDa
  • 表达区域：
    21-395aa
  • 氨基酸序列
    EVTSDQVANVVWDYFTQLSNNAKEAVEQFQKTDVTQQLSTLFQDKLGDASTYADGVHNKLVPFVVQLSGHLAQETERVKEEIKKELEDLRDRMMPHANKVTQTFGENMQKLQEHLKPYAVDLQDQINTQTQEMKLQLTPYIQRMQTTIKENVDNLHTSMMPLATNLKDKFNRNMEELKGHLTPRANELKATIDQNLEDLRRSLAPLTVGVQEKLNHQMEGLAFQMKKNAEELQTKVSAKIDQLQKNLAPLVEDVQSKVKGNTEGLQKSLEDLNRQLEQQVEEFRRTVEPMGEMFNKALVQQLEQFRQQLGPNSGEVESHLSFLEKSLREKVNSFMSTLEKKGSPDQPQALPLPEQAQEQAQEQAQEQVQPKPLES
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of this Recombinant Mouse Apoa4 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the Apoa4 protein could be inserted into a vector and introduced into an expression host, Yeast, where it could be easily expressed in and purified from. The expression of this Apoa4 protein was at 21-395aa. N-terminal 6xHis tag was fused with this protein. The purity is 90%+ determined by SDS-PAGE.

    Apoa4 is gene encoding a protein named apolipoprotein A-IV (Apoa4, Apo-AIV or ApoA-IV) or apolipoprotein A4. The protein encoded by this gene, a well-known short-term satiating protein, is highly present in by the gut and the brain in the presence of dietary lipids. Emerging evidence has reported that ApoA4 shows a S1P-binding property comparable to albumin, and ApoA4-bound S1P activated S1P receptors efficiently. ApoA4 can be a novel S1P carrier protein.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
  • 基因功能参考文献：
    1. This study uncovers an anti-sense lncRNA (APOA4-AS), which is co-expressed with APOA4, and concordantly and specifically regulates APOA4 expression both in vitro and in vivo with the involvement of HuR. PMID: 27131369
    2. Our findings may throw light on the function of ApoA4 in inflammatory responses and acute-phase reactions, as well as the development of SERPINA3 relative diseases. PMID: 28412351
    3. Very Low Density Lipoprotein (VLDL) assembly and CREBH activation play key roles in the response to hepatic steatosis by up-regulating apoA-IV and promoting assembly and secretion of larger, more triglyceride - enriched VLDL particles. PMID: 27655915
    4. It was suggested that increased ileal GPR119 is a potential mechanism by which GLP-1 secretion is enhanced in apoA-IV-/- mice. PMID: 26294669
    5. these data suggest that apoA-IV is not necessary for the metabolic improvements shown with VSG, but also suggest an interesting role for apoA-IV in regulating macronutrient preference and hepatic triglyceride levels. PMID: 25157093
    6. These results reveal ApoA-IV as a novel follicle-associated epithelium-specific marker especially in the upper small intestine of adult mice. PMID: 24838500
    7. transcriptional regulation of apolipoprotein A-IV by the transcription factor CREBH PMID: 24598141
    8. apoA-IV inhibits hepatic gluconeogenesis by decreasing Glc-6-Pase and PEPCK gene expression through NR1D1. PMID: 24311788
    9. Hepatic steatosis in mice induces hepatic apoA-IV expression, which in turn promotes lipoprotein particle expansion and reduces hepatic lipid burden without increasing the number of secreted atherogenic apoB-containing lipoprotein particles. PMID: 24030551
    10. Data indicate that plasma lipids, lipid absorption, and microsomal triglyceride transfer protein (MTP), FoxO1, and FoxA2 levels are lower at night and at mealtime in apoAIV-/- mice. PMID: 23729668
    11. a novel function of apoA-IV in the biogenesis of discrete HDL-A-IV particles with the participation of ABCA1 and LCAT PMID: 23132909
    12. peripheral apo AIV requires an intact CCK system and vagal afferents to activate neurons in the hindbrain to reduce food intake PMID: 23027805
    13. results suggest that apoA-IV may provide a therapeutic target for the regulation of glucose-stimulated insulin secretion and treatment of diabetes PMID: 22619326
    14. Data show that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs). PMID: 22209087
    15. These data suggest that Apoa4 has a previously unknown role in mediating the metabolism of chylomicrons, and therefore may be important in regulating plasma lipid metabolism. PMID: 22207575
    16. apoA-IV may play a unique role in integrating feeding behavior, intestinal lipid absorption, and energy storage PMID: 21840868
    17. ApoA-IV deficiency increases Abeta deposition and results in cognitive damage in the mouse model. PMID: 21356380
    18. ApoA-IV restriction to enterocyte is controlled by a new hormone-response element. PMID: 12105231
    19. Results provide the first direct support for the hypothesis that apolipoprotein A-IV is an endogenous anti-inflammatory protein. PMID: 15254593
    20. apo A-IV and apo A-V are positive acute-phase proteins in mouse HDL PMID: 15306172
    21. results demonstrate that apoA-IV is a direct ERRalpha target gene and suggest a function for ERRalpha in intestinal fat transport, a crucial step in energy balance PMID: 15466464
    22. the induction of apoA-IV expression in fasting and diabetes likely involves PGC-1 alpha-mediated coactivation of HNF-4 alpha in addition to glucocorticoid-dependent actions PMID: 16929032
    23. The hypothalamic apo A-IV is regulated by leptin, at least partially, via the STAT3 signaling pathway. PMID: 17363460
    24. Role for apo A-IV and CCK(1)R in PYY(3-36)-induced activation of vagal afferent pathway and inhibition of gastric emptying, but this is likely not pathway mediating effects of PYY(3-36) on food intake. PMID: 17641001
    25. the apoCIII enhancer contributes to the maintenance of an active chromatin subdomain of the apoAI/CIII/AIV genes, but not apoAV PMID: 18678879

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 组织特异性：
    Secreted in plasma.
  • 数据库链接：

    KEGG: mmu:11808

    STRING: 10090.ENSMUSP00000034585

    UniGene: Mm.4533