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  4. Recombinant Mouse Amiloride-sensitive sodium channel subunit beta (Scnn1b), partial

Recombinant Mouse Amiloride-sensitive sodium channel subunit beta (Scnn1b), partial

  • 中文名称：
    小鼠Scnn1b重组蛋白
  • 货号：
    CSB-YP895745MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Scnn1b重组蛋白
  • 货号：
    CSB-EP895745MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Scnn1b重组蛋白
  • 货号：
    CSB-EP895745MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Scnn1b重组蛋白
  • 货号：
    CSB-BP895745MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Scnn1b重组蛋白
  • 货号：
    CSB-MP895745MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Scnn1b
  • Uniprot No.：
  • 别名：
    Scnn1bAmiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na(+) channel subunit beta; Beta-ENaC; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • 基因功能参考文献：
    1. Scnn1b-Tg mice exhibited an approximately 50% reduction mucociliary clearance PMID: 28435155
    2. Loss of betaENaC function resulted in meibomian gland disease and severe ocular surface damage that phenocopied aspects of human pseudohypoaldosteronism-1 MG disease and was sex dependent. PMID: 29107074
    3. Nedd4-2-mediated ubiquitination of beta-ENaC leading to endocytosis and degradation of apical Na(+) channels is a key feature of hypoxia-induced inhibition of transepithelial alveolar Na(+) transport. PMID: 24093724
    4. Our findings are consistent with the role of betaENaC as a vascular smooth muscle cell mechanosensor and function of evolutionarily related nematode degenerin proteins. PMID: 23552864
    5. Cys substitutions at sites in the wrist domains of the beta and gamma subunits alter the Na+ self-inhibition response. PMID: 23144453
    6. These findings demonstrate that myogenic constriction in afferent arterioles is dependent on normal expression of betaENaC PMID: 22419697
    7. loss of beta-ENaC associated with early signs of renal injury and increased mean arterial pressure PMID: 21543417
    8. generation of transgenic mice with airway-specific overexpression of the epithelial Na(+) channel (ENaC) is an alternative approach to mimic cystic fibrosis ion transport pathophysiology in the lung PMID: 21658636
    9. Airway surface liquid volume regulation determines different airway phenotypes in liddle compared with betaENaC-overexpressing mice. PMID: 20566636
    10. Il-6 activates ENaC-beta channels in cortical collecting duct cells. PMID: 20504903
    11. Beta-ENaC is an important mediator of renal myogenic constriction-mediated renal blood flow autoregulation in vivo. PMID: 19889952
    12. increase in whole cell current is attributed to a difference in the density of conducting channels with Liddle mutation in kidney PMID: 12684224
    13. beta ENaC is a substrate for methylation in mouse cortical collecting duct cells PMID: 16469734
    14. Mice heterozygous for beta-ENaC have a small but detectable defect in their ability to conserve Na+ and a more readily apparent defect in the ability to secrete K+. PMID: 16571596
    15. beta and gammaENaC proteins are essential in mediating myogenic vasoconstriction in mouse renal interlobar arteries PMID: 16849693
    16. Constitutive ENaC activation improved sodium-driven alveolar fluid clearance in the mouse lung, and attenuated the severity of hydrostatic pulmonary oedema. PMID: 17430990
    17. both mouse and human mammary cells express all ENaC subunits, and they are regulated by steroid hormones in a temporal and cell-specific manner both in culture and in vivo PMID: 17510235
    18. Epidermal growth factor inhibits sodium absorption via an ERK1/2-mediated down-regulation of Scnnb1. PMID: 17724164
    19. Aldosterone-induced transcriptional up-regulation of the channel's beta- and gamma-subunit (beta ENaC and gamma ENaC) and of the serum- and glucocorticoid-inducible kinase 1 (SGK1) was similar in colon tissue from Liddle and wild-type animals. PMID: 18006588
    20. despite the compensatory increase in alpha- and gamma-ENaC protein expression observed in mutant mouse lung, low expression of beta-ENaC results in a moderate impairment of baseline alveolar fluid clearance and in decreased AFC sensitivity to amiloride. PMID: 18024719
    21. Amiloride administration did not have benefits if treatment was started after the development of CF-like lung disease in betaENaC-overexpressing mice. PMID: 18849497
    22. There is a critical role of ENaC in the in vivo pathogenesis of cystic fibrosis (CF) lung disease and amiloride may be an effective preventive therapy for CF patients. PMID: 19060118
    23. Middle cerebral artery pressure-induced constriction is inhibited in a mouse model of reduced betaENaC. PMID: 19553501

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, SCNN1B subfamily
  • 组织特异性：
    Lung and kidney.
  • 数据库链接：

    KEGG: mmu:20277

    STRING: 10090.ENSMUSP00000033161

    UniGene: Mm.7709