Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Alpha-tubulin N-acetyltransferase (Atat1)

Recombinant Mouse Alpha-tubulin N-acetyltransferase (Atat1)

  • 货号：
    CSB-YP812982MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP812982MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP812982MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP812982MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP812982MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Atat1
  • Uniprot No.：
  • 别名：
    Atat1; Mec17Alpha-tubulin N-acetyltransferase 1; Alpha-TAT; Alpha-TAT1; TAT; EC 2.3.1.108; Acetyltransferase mec-17 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-421
  • 氨基酸序列
    MEFPFDVDAL FPERITVLDQ HLRPPARRPG TTTPARVDLQ QQIMTIVDEL GKASAKAQHL PAPITSALRM QSNRHVIYIL KDTSARPAGK GAIIGFLKVG YKKLFVLDDR EAHNEVEPLC ILDFYIHESV QRHGHGRELF QHMLQKERVE PHQLAIDRPS PKLLKFLNKH YNLETTVPQV NNFVIFEGFF AHQHRPPTSS LRATRHSRAA VADPIPAAPA RKLPPKRAEG DIKPYSSSDR EFLKVAVEPP WPLNRAPRRA TPPAHPPPRS SSLGNSPDRG PLRPFVPEQE LLRSLRLCPP HPTARLLLAT DPGGSPAQRR RTRGTPWGLV AQSCHYSRHG GFNTSFLGTG NQERKQGEQE AEDRSASEDR VLLLDGSGEE PTQTGAPRAQ APPPQSWTVG GDIMNARVIR NLQERRSTRP W
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
  • 基因功能参考文献：
    1. TAK1 directly interacts with and phosphorylates alphaTAT1 at Ser237 to critically enhance its catalytic activity. PMID: 29703898
    2. Atat1 knockdown resulted in a significant increase in the expression of ACTH-producing genes and decreased the dexamethasone-induced nuclear translocation of glucocorticoid receptor accompanied with a reduction in alpha-tubulin acetylation. PMID: 28687926
    3. Here the s demonstrate that mice lacking the alpha-tubulin acetyltransferase Atat1 in sensory neurons display profound deficits in their ability to detect mechanical stimuli. PMID: 27976998
    4. alphaTAT1 has a conserved function as the major alpha-tubulin acetyltransferase in ciliated organisms and has an important role in regulating subcellular specialization of subsets of microtubules. PMID: 23748901
    5. Data indicate that alpha-tubulin acetyltransferase 1 Atat1 is not required for survival and development but may regulate more advanced functions. PMID: 23720746
    6. Acetylation of alpha-tubulin is under the control of the acetyltransferase MEC-17 and deacetylases SIRT2 (Sirtuin 2) and HDAC6 (histone deacetylase 6). Adipocyte development is inhibited in MEC-17-knockdown cells, but enhanced in MEC-17-overexpressing cells. PMID: 23126280

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Membrane, clathrin-coated pit. Cell junction, focal adhesion. Cell projection, axon. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Acetyltransferase ATAT1 family
  • 组织特异性：
    Widely expressed with highest levels in neuronal tissues. In the brain, expressed in the cortex, cerebellum and hippocampus, including the pyramidal layers in CA1 and CA3, as well as the granular cell layers in the lateral blade (suprapyramidal portion) a
  • 数据库链接：

    KEGG: mmu:73242

    STRING: 10090.ENSMUSP00000056383

    UniGene: Mm.273155