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  4. Recombinant Mouse Alcohol dehydrogenase class-3 (Adh5)

Recombinant Mouse Alcohol dehydrogenase class-3 (Adh5)

  • 中文名称：
    Recombinant Mouse Alcohol dehydrogenase class-3(Adh5),Yeast
  • 货号：
    CSB-YP001357MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Mouse Alcohol dehydrogenase class-3(Adh5),Yeast
  • 货号：
    CSB-EP001357MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Mouse Alcohol dehydrogenase class-3(Adh5),Yeast
  • 货号：
    CSB-EP001357MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Mouse Alcohol dehydrogenase class-3(Adh5),Yeast
  • 货号：
    CSB-BP001357MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Mouse Alcohol dehydrogenase class-3(Adh5),Yeast
  • 货号：
    CSB-MP001357MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Adh5
  • Uniprot No.：
  • 别名：
    Adh5; Adh-2; Adh2; Alcohol dehydrogenase class-3; EC 1.1.1.1; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; ADH-B2; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; EC 1.1.1.-; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.284
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-374
  • 氨基酸序列
    ANQVIRCKA AVAWEAGKPL SIEEIEVAPP KAHEVRIKIL ATAVCHTDAY TLSGADPEGC FPVILGHEGA GIVESVGEGV TKLKAGDTVI PLYIPQCGEC KFCLNPKTNL CQKIRVTQGK GLMPDGTSRF TCKGKSVFHF MGTSTFSEYT VVADISVAKI DPSAPLDKVC LLGCGISTGY GAAVNTAKVE PGSTCAVFGL GGVGLAVIMG CKVAGASRII GIDINKDKFA KAKEFGASEC ISPQDFSKSI QEVLVEMTDG GVDYSFECIG NVKVMRSALE AAHKGWGVSV VVGVAASGEE ISTRPFQLVT GRTWKGTAFG GWKSVESVPK LVSEYMSKKI KVDEFVTGNL SFDQINQAFD LMHSGDSIRT VLKM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol.
  • 基因功能参考文献：
    1. Site-directed mutagenesis of Akt at Cys224 revealed that S-nitrosylation at this site was pivotal for the reduced phosphorylation at Akt Ser473, which led to impaired Akt signaling. Furthermore, on HHcy challenge, as compared with GSNOR(+/+)ApoE(-/-) littermate controls, GSNOR(-/-)ApoE(-/-) double knockout mice showed reduced T-cell activation with concurrent reduction of atherosclerosis. PMID: 29860106
    2. Data (including data from studies using knockout and transgenic mice) suggest that obesity and diabetes are accompanied by decreases in GSNOR activity in hepatocytes engendering nitrosative stress; obesity promotes S-nitrosylation of lysosomal proteins in liver, thereby impairing lysosomal enzyme activities and compromising autophagy. PMID: 29074597
    3. Report inhibition of GSNOR activity by nebivolol leading to accumulation of nitrosothiols in cells, and this is associated with an enhanced vasodilation by S-nitrosoglutathione. PMID: 26746429
    4. Increased GSNOR expression during aging decreases S-nitrosation of CaMKIIalpha and reduces CaMKIIalpha synaptosomal accumulation. PMID: 28883020
    5. These data indicate a role for GSNOR in the host response to malaria infection and suggest that strategies to disrupt its activity will improve clinical outcomes. PMID: 28674030
    6. GSNOR may act as a "brake" on skeletal muscle contractile performance under physiological conditions by modulating nitrosylation/denitrosylation balance. PMID: 27412893
    7. Loss of GSNOR confers enhanced post-MI cardiac regenerative activity, characterized by enhanced turnover of cardiomyocytes and cardiac stem cells. PMID: 26178404
    8. Results show that ADH5 removes endogenous formaldehyde to prevent DNA adducts, and protects with FANCD2, hematopoietic stem cells, hepatocytes, and nephrons from endogenous DNA damage. PMID: 26412304
    9. S-nitrosoglutathione reductase-dependent modification of PPARgamma alters the balance between adipocyte and osteoblast differentiation and provides checkpoint regulation of the lineage bifurcation of these 2 lineages. PMID: 25798618
    10. These findings provide novel insights into the involvement of GSNOR and S-nitrosylation in neuromuscular atrophy and neuropathic pain that are associated with pathological states. PMID: 24684653
    11. Overexpression of ADH5 reduces both development and adult neuronal differentiation of neurons. This effect depends on the catalytic activity of ADH5 and involves ADH5-mediated denitrosation of histone deacetylase 2 (HDAC2). PMID: 24895131
    12. GSNOR appears to play a crucial role in controlling pulmonary and systemic infection by K. pneumoniae. PMID: 24239886
    13. A critical role for GSNOR in maintaining genomic integrity. PMID: 23354311
    14. This study suggests that S-nitrosoglutathione reductase degradation of S-nitrosoglutathione is a vital step in the expression of ventilatory roll-off PMID: 23183419
    15. GSNOR deficiency has a role in hepatocarcinogenesis, which is prevented by iNOS inhibition PMID: 23440427
    16. Increasing the denitrosylation capacity of cardiomyocytes, via cardiomyocyte-specific overexpression of GSNOR, protects against sepsis-induced myocardial depression. PMID: 23417863
    17. Compared the protein expression profiles in the livers of wild-type and GSNOR-deficient (GSNOR(-/-) ) mice that were challenged with lipopolysaccharide; subsequently id'd 19 upregulated and 19 downregulated proteins in GSNOR(-/-) mice using mass spec. PMID: 22623366
    18. These results indicate that systemic hemodynamic responses (vascular tone and cardiac contractility), both under basal conditions and after adrenergic activation, are regulated through concerted actions of NO synthase/GSNOR. PMID: 22366318
    19. protection of AGT and resistance to nitrosamine-induced genotoxicity critically depends on GSNOR in hepatocytes PMID: 21385828
    20. Data show that GSNO-R activity was elevated in lung homogenates from female compared to male mice. PMID: 21103380
    21. GSNOR denitrosylates connexin 43 at the gap junctions between vascular epithelial and vascular smooth muscle cells. PMID: 21071693
    22. GSNOR, likely through regulation of S-nitrosylation and apoptosis, physiologically plays a protective role in the development of the immune system. PMID: 20980633
    23. Data suggest that GSNOR deficiency, through dysregulated S-nitrosylation, may promote hepatocellular carcinoma, possibly by inactivating a DNA repair system. PMID: 20371487
    24. following allergen challenge, wild-type mice exhibiting airway hyperresponsivity have increased airway levels of the enzyme GSNO reductase (GSNOR) and are depleted of lung S-nitrosothiols (SNOs) PMID: 15919956
    25. Mechanisms are presented through which nitric acid and S-glutathione mediate nitrosative stress, as well as the cellular pathways of protein S-nitrosylation and denitrosylation involving GSNO reductase. PMID: 19138101
    26. GSNOR inhibitors to be novel tools for regulating nitric oxide bioactivity and assessing the role of SNOs in vivo. PMID: 19596685

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Zinc-containing alcohol dehydrogenase family, Class-III subfamily
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:11532

    STRING: 10090.ENSMUSP00000005964

    UniGene: Mm.3874