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  4. Recombinant Mouse Acid sphingomyelinase-like phosphodiesterase 3b (Smpdl3b)

Recombinant Mouse Acid sphingomyelinase-like phosphodiesterase 3b (Smpdl3b)

  • 中文名称：
    小鼠Smpdl3b重组蛋白
  • 货号：
    CSB-YP021850MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Smpdl3b重组蛋白
  • 货号：
    CSB-EP021850MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Smpdl3b重组蛋白
  • 货号：
    CSB-BP021850MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Smpdl3b重组蛋白
  • 货号：
    CSB-MP021850MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Smpdl3b
  • Uniprot No.：
  • 别名：
    Smpdl3b; Asml3bAcid sphingomyelinase-like phosphodiesterase 3b; ASM-like phosphodiesterase 3b; EC 3.1.4.-
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-456
  • 氨基酸序列
    QL GRFWHISDLH LDPNYTVSKD PLQVCPSAGS QPVLNAGPWG DYLCDSPWAL INSSLYAMKE IEPKPDFILW TGDDTPHVPN ESLGEAAVLA IVERLTNLIK EVFPDTKVYA ALGNHDFHPK NQFPAQSNRI YNQVAELWRP WLSNESYALF KRGAFYSEKL PGPSRAGRVV VLNTNLYYSN NEQTAGMADP GEQFRWLGDV LSNASRDGEM VYVIGHVPPG FFEKTQNKAW FRESFNEEYL KVIQKHHRVI AGQFFGHHHT DSFRMFYDNT GAPINVMFLT PGVTPWKTTL PGVVDGANNP GIRIFEYDRA TLNLKDLVTY FLNLRQANVQ ETPRWEQEYR LTEAYQVPDA SVSSMHTALT RIASEPHILQ RYYVYNSVSY NHLTCEDSCR IEHVCAIQHV AFNTYATCLH GLGAKLVPGF LLILTLLPSL HVLEVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
  • 基因功能参考文献：
    1. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
    2. Although a potential substrate for SMPDL3B is sphingomyelin, we identify other possible substrates such as CDP-choline, ATP, and ADP. PMID: 27687724
    3. our results identify the membrane-modulating enzyme SMPDL3B as a negative regulator of TLR signaling that functions at the interface of membrane biology and innate immunity. PMID: 26095358
    4. SMPDL3b may be an important modulator of podocyte function by shifting suPAR-mediated podocyte injury from a migratory phenotype to an apoptotic phenotype and that it represents a novel therapeutic glomerular disease target PMID: 24925721
  • 亚细胞定位：
    Secreted. Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Acid sphingomyelinase family
  • 组织特异性：
    Macrophages and dendritic cells.
  • 数据库链接：

    KEGG: mmu:100340

    STRING: 10090.ENSMUSP00000030709

    UniGene: Mm.287187