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  4. Recombinant Mouse ADP-ribosylation factor-binding protein GGA3 (Gga3)

Recombinant Mouse ADP-ribosylation factor-binding protein GGA3 (Gga3)

  • 货号：
    CSB-YP804375MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP804375MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP804375MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP804375MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP804375MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gga3
  • Uniprot No.：
  • 别名：
    Gga3; Kiaa0154ADP-ribosylation factor-binding protein GGA3; Golgi-localized; gamma ear-containing; ARF-binding protein 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-718
  • 氨基酸序列
    MAEAEGESLE SWLNKATNPS NRQEDWEYII GFCDQINKEL EGPQIAVRLL AHKIQSPQEW EAVQALTVLE ACMKNCGRRL HNEVGKFRFL NELIKVVSPK YLGDRVSEKV KTKVIELLFS WTLALPEEAK IKDAYHMLKR QGIVQSDPPI PMDRTLIPSP PPRPKNPVFD DEEKSKLLAR LLKSKNPDDL QEANRLIKSM VKEDEARIQK VTKRLHTLEE VNNNVKLLHE MLLHYSQEYS SDADKELMKE LFDRCENKRR TLFKLASETE DNDNSLGDIL QASDNLSRVI NSYKTIIEGQ IVNGEVTTST MPDSEGNSHC GNQGALIDLA ELDAPSNSSP ALAPPTSGIP ILPPPPQTSG PPRSRSSSQA EAPPGSDSTN NALSLLDEEL LCLGLTDPAP TAPKESPGSS QWHLFQNEPP SDLDFFSPRP VPAASCPSDG PQLPPPVSTS SMSQAPLPAA FPAPVVPASA PTHSTGSFMF SSGPAPALAP KAEPKGPEYP SSSTSHRLDA LDQLLEEAKV TSGLVKPVSC FSPGPTASPL LPASAPARPL LPFSTGPGSP LFQSQGSPQK GPELSLASVH VPLESIKPSS ALPVTAYDKN GFRILFHFAK ECPPGRPDVL VVVVSMLNTA PLPVKSIVLQ AAVPKSMKVK LQPPSGTELS PFSPIQPPAA ITQVMLLANP MKEKVRLRYK LTFALGEQLS TELGEVDQFP PVEQWGNL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif.; Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Involved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels independently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane.
  • 基因功能参考文献：
    1. GGA3 plays a key role in GABAergic transmission. GGA3 deletion results in decreased anxiety-like behaviors and increased BACE1 levels. PMID: 27192432
    2. depletion of GGA1 and GGA3 engender a rapid and robust elevation of BACE1 in the acute phase after brain injury. However, the efficient disposal of the acutely accumulated BACE1 solely depends on GGA3 levels in the subacute phase of injury PMID: 22836275
    3. GGA3 tightly and inversely regulate BACE1 levels via interaction with ubiquitin sorting machinery PMID: 20484053
  • 亚细胞定位：
    Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    GGA protein family
  • 数据库链接：

    KEGG: mmu:260302

    STRING: 10090.ENSMUSP00000019135

    UniGene: Mm.119479