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  4. Recombinant Mouse 5-AMP-activated protein kinase subunit beta-1 (Prkab1)

Recombinant Mouse 5-AMP-activated protein kinase subunit beta-1 (Prkab1)

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  • 中文名称：
    小鼠Prkab1重组蛋白
  • 货号：
    CSB-EP889918MO
  • 规格：
    ￥2328
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkab15'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    36.1 kDa
  • 表达区域：
    2-270aa
  • 氨基酸序列
    GNTSSERAALERQAGHKTPRRDSSGGAKDGDRPKILMDSPEDADIFHSEEIKAPEKEEFLAWQHDLEANDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYMSKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
  • 基因功能参考文献：
    1. activation of AMPK at early stage of adipogenesis is involved in the anti-adipogenesis effect of Red Pepper Seed extract. PMID: 29316805
    2. in the absence of the tumor suppressor p53, germline genetic deletion of AMPK beta1 accelerates the appearance of a T-cell lymphoma that reduces lifespan compared to p53 deficiency alone. PMID: 28544264
    3. In lipid-laden macrophages, Ampk activation decreased cholesterol content (foam cell formation) and increased cholesterol efflux to HDL and apoA-I, effects that occurred in an Ampk beta1-dependent manner. PMID: 25773887
    4. Salicylate activates the AMP-activated protein kinase (AMPK) by binding at the A-769662 drug binding site on the AMPK beta1-subunit PMID: 25940306
    5. AMPK beta1beta2 have a role in preventing myopathy due to loss of capillary density in nonpostural muscles PMID: 24522207
    6. The aim of this study was to determine whether activation of AMPK by acute renal ischemia influences the severity of renal ischemia-reperfusion injury. PMID: 22253816
    7. AMPK beta1 protects macrophages from inflammation under high lipid exposure from a high fat diet. beta1(-/-) macrophages displayed increased levels of diacylglycerol and markers of inflammation. PMID: 22080866
    8. cisplatin-triggered activation of AMPK and subsequent suppression of mTOR activity can induce an autophagic response that protects tumour cells from cisplatin-mediated apoptotic death PMID: 20196784
    9. Data show that beta1beta2M-KO mice are physically inactive and have a drastically impaired capacity for treadmill running that is associated with reductions in skeletal muscle mitochondrial content. PMID: 21896769
    10. Phosphorylation of AMPK by Ulk1 represents a negative feedback circuit. PMID: 21460634
    11. AMPK negatively regulates Nox4-dependent activation of p53 and podocytes apoptosis in diabetes. PMID: 20861022
    12. AMPK negatively regulates lipid-induced inflammation, which acts through SIRT1, thereby contributing to the protection against obesity, inflammation, and insulin resistance PMID: 20421294
    13. Germline deletion of either AMPK beta1 or beta2 subunit isoforms resulted in reduced trabecular bone density and mass, but without effects on osteoclast (OC) or osteoblast (OB) numbers. PMID: 19723702
    14. The loss of AMPK beta1 reduces food intake and protects against the deleterious effects of an obesity-inducing diet. PMID: 19892703
    15. metformin and AMPK have a previously unrecognized role in regulating the circadian rhythm PMID: 17525164
    16. regulation of FOXO3 by AMPK may play a crucial role in fine tuning gene expression programs that control energy balance and stress resistance in cells throughout life PMID: 17711846
    17. metformin-mediated AMPK activation leads to inhibition of mTOR and a reduction in translation initiation PMID: 18006825
    18. AMPK activation appears to restrain the energy depletion and oxidative stress caused by lipolysis PMID: 18390901
    19. Regulation of erythrocyte survival by AMPK is reported. PMID: 19050047
    20. AMPK integrates growth factor signaling with cell cycle control to regulate brain development PMID: 19217427

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  • 蛋白家族：
    5'-AMP-activated protein kinase beta subunit family
  • 数据库链接：

    KEGG: mmu:19079

    STRING: 10090.ENSMUSP00000031486

    UniGene: Mm.458152