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  4. Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin

Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin

  • 中文名称：
    Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin,Yeast
  • 货号：
    CSB-YP332662LDT
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin,Yeast
  • 货号：
    CSB-EP332662LDT
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin,Yeast
  • 货号：
    CSB-EP332662LDT-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin,Yeast
  • 货号：
    CSB-BP332662LDT
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin,Yeast
  • 货号：
    CSB-MP332662LDT
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Chlorotoxin; CTX; ClTx; Tozuleristide
  • 种属：
    Leiurus quinquestriatus quinquestriatus (Egyptian scorpion)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    1-36
  • 氨基酸序列
    MCMPCFTTDH QMARKCDDCC GGKGRGKCYG PQCLCR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP332662LDT, could you please provide some informations ?
Have you expressed this protein before? If so, for which hosts? I was specifically interested in a His-tagged protein from E. coli.
Are you able to tell us which strain of E. coli is used for production?

A:
Very nice to receive your inquiry.
Recombinant Leiurus quinquestriatus quinquestriatus Chlorotoxin
CSB-EP332662LDT >> E.coli
Expression Region: 1-36aa;Full length;
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MCMPCFTTDHQMARKCDDCCGGKGRGKCYGPQCLCR


We have not expressed this protein before. As this protein is relatively small, we suggest to use a larger tag for E.coli expression system, such as N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. We could also try to provide His-tag if you need. Please remark when you send the official order. The strain of E.coli that we usually use is Rosetta and BL21(DE3).

靶点详情

  • 功能：
    This toxin binds to the surface of glioma cells, and inhibits their proliferation without having effects on normal brain cells. In this context, this toxin has been described as a chloride channel inhibitor (probably ClC-3/CLCN3) by causing its internalization via caveolae. It has also been described to selectively interact with MMP2 (in complex with MT1-MMP (MMP14) and TIMP2), to inhibit its enzymatic activity and to decrease its presence at the cell surface.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Short scorpion toxin superfamily, Chloride channel inhibitor family
  • 组织特异性：
    Expressed by the venom gland.