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  4. Recombinant JC polyomavirus Agnoprotein, partial

Recombinant JC polyomavirus Agnoprotein, partial

  • 货号：
    CSB-YP355945JAK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP355945JAK
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP355945JAK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP355945JAK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP355945JAK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Agnoprotein; Agno
  • 种属：
    JC polyomavirus (JCPyV) (JCV)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Alters the structure of the nuclear envelope by interacting with host CBX5 and disrupting CBX5 association with LBR. Involved in the perinuclear-nuclear localization of the capsid protein VP1 during virion assembly and maturation. Plays an important role in the release of progeny virions from infected cells and in viral propagation, probably by acting as a viral ionic channel in the host plasma membrane. Allows influx of extracellular calcium ions in the host cell. May contribute to viral genome transcription and translation of viral late proteins.
  • 基因功能参考文献：
    1. the resolution of the 3D structure of full-length JCV agnoprotein by NMR, which not only confirmed the existence of the previously reported major alpha-helix domain at the same position but also revealed the presence of an additional minor alpha-helix region spanning amino acid residues Leu6 to lys13. The remaining regions of the protein adopt an intrinsically unstructured conformation. PMID: 28295503
    2. Amino acid residues (Lys22, Lys23, Phe31, Glu34, and Asp38) located either on or adjacent to the hydrophilic surface of the major alpha-helix domain of Agno play critical roles in release. PMID: 28722139
    3. L29A and L36A mutations of agnoprotein exhibited a significant negative effect on both protein stability and viral replication PMID: 24672035
    4. s showed that a Leu/Ile/Phe-rich domain, (spanning aa 28-39) within alpha-helix is indispensable for dimer/oligomer formation and stable expression of the protein and that residues 34-36 are involved in regulation of viral transcripts splicing. PMID: 23747198
    5. Agnoprotein affects virion morphology and the virions of the agnoprotein deletion mutant are irregularly shaped. PMID: 22708997
    6. JC virus agnoprotein enhances large T antigen binding to the origin of viral DNA replication. PMID: 22840425
    7. The novel tropism for infection of cortical pyramidal neurons in JC virus encephalopathy may be associated with an JC virus agnoprotein deletion mutation. PMID: 22536439
    8. Agnoprotein forms homodimer/oligomer complexes and these are important for its function during viral propagation. PMID: 21920573
    9. Infection by agnoprotein-negative mutants of both polyomavirus JC and SV40 virus results in the release of virions that are mostly deficient in DNA content. PMID: 21609431
    10. we demonstrate that agnoprotein possesses properties commonly associated with viroporins PMID: 20300659
    11. These observations provide the first evidence for the possible involvement of agnoprotein in a series of biological events that may contribute to the pathological features seen in progressive multifocal leukoencephalopathy lesions. PMID: 17989177

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  • 亚细胞定位：
    Host cytoplasm. Host nucleus membrane; Single-pass type II membrane protein. Host rough endoplasmic reticulum membrane; Single-pass type II membrane protein. Host cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Polyomavirus agnoprotein family
  • 数据库链接：

    KEGG: vg:1489519