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  4. Recombinant Insulin-like receptor (daf-2), partial

Recombinant Insulin-like receptor (daf-2), partial

  • 中文名称：
    Recombinant Insulin-like receptor(daf-2),partial,Yeast
  • 货号：
    CSB-YP853367CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Insulin-like receptor(daf-2),partial,Yeast
  • 货号：
    CSB-EP853367CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Insulin-like receptor(daf-2),partial,Yeast
  • 货号：
    CSB-EP853367CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Insulin-like receptor(daf-2),partial,Yeast
  • 货号：
    CSB-BP853367CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Insulin-like receptor(daf-2),partial,Yeast
  • 货号：
    CSB-MP853367CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    daf-2
  • Uniprot No.：
  • 别名：
    daf-2; Y55D5A.5; Insulin-like receptor; IR; EC 2.7.10.1; Abnormal dauer formation protein 2) [Cleaved into: Insulin-like receptor subunit alpha; Insulin-like receptor subunit beta]
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Insulin receptor-like tyrosine kinase which regulates metabolism, controls longevity and prevents developmental arrest at the dauer stage. Binding of INS family members may either stimulate, or antagonize, association of the receptor with downstream mediators such as pdk-1 and age-1. Required for germline progenitor proliferation during larval development. Required for the response to environmental stimuli such as light, food, pheromone, and temperature. Negatively regulates resistance to UV and oxidative stress. In a daf-16/FOXO-dependent manner, plays a role in regulating the response to white light. Role in immune function and pathogen resistance. Negatively regulates autophagy. Regulates daf-18/PTEN protein levels. Plays a role in controlling seam cell development during the larval stages.
  • 基因功能参考文献：
    1. neuron-class-specific alternative splicing determined by the combinatorial action of RBFOX proteins (ASD-1 and FOX-1), UNC-75/CELF and PTB-1/PTB PMID: 27198602
    2. Suggest mbk-1 as a novel regulator of C. elegans longevity upon both, germline ablation and DAF-2 inhibition, and provide evidence for mbk-1 regulating DAF-16 activity in germline-deficient animals. PMID: 28562327
    3. Five MMP alleles proved to be novel dauer-enhancing mutations, including one new allele in the previously uncharacterized C-terminus of DAF-2 CRISPR-Cas9 genome editing was used to confirm the phenotypic consequence of six of these DAF-2 mutations and to replicate an allelic series of known human disease mutations in a highly conserved tyrosine kinase active site residue PMID: 27856697
    4. Cholesterol is required for the fasting-induced upregulation of transcriptional target genes such as the insulin/IGF-1 pathway effector DAF-16, and cholesterol deprivation suppresses the long lifespan of the insulin/IGF-1 receptor daf-2 mutant. Remarkably, we found that cholesterol plays an important role in the fasting-induced nuclear accumulation of DAF-16. PMID: 27989925
    5. this study shows that heat shock increases the life span of wild type Caenorhabditis elegans upon pathogenic encounter; similar increase in resistance towards pathogenesis observed in long-lived C. elegans daf-2 mutants PMID: 27317398
    6. this study shows that daf-2(-) mutants are hyper-immune and exhibit reduced pathological consequences during challenge with Coxiella burnetii PMID: 27884946
    7. Results indicates a contribution of daf-2-mediated insulin signalling in the transition from local area search to dispersal in food-associated behaviour in adult C. elegans PMID: 27209024
    8. The long-lived mutant daf-2(m41) worms are metabolically youthful compared to wild-type worms, but this relative youth only appears in middle age. PMID: 26381038
    9. daf-2(e1370) mutation improves quality of life and lifespan extension. PMID: 26586186
    10. These results reveal a new function for daf-2 signaling, and, most importantly, demonstrate that genetic programming contributes to freezing survival. PMID: 26635120
    11. Insulin signalling regulates gene expression during cold tolerance. Insulin released from pheromone-sensing neurons is received by the intestine and neurons regulating gene expression for cold habituation. PMID: 25048458
    12. When daf-2 animals were incubated at 20 degrees C they remained long-lived, but nuclear appearance changed at similar rate as in the wild type. PMID: 24671263
    13. Integrated our proteomic data set together with a selection of other data in an attempt to reconstruct and resolve the intricacies of altered daf-2 metabolism. PMID: 24555535
    14. in the presence of food, the DAF-2 insulin-like receptor signals through the RAS-ERK pathway to drive meiotic prophase I progression and oogenesis; in the absence of food, there is downregulation of the RAS-ERK pathway, and oogenesis is stalled. PMID: 24120884
    15. Reconciliation of daf-2 suppression by elt-3 in Caenorhabditis elegans from Tonsaker et al. (2012) and Kim et al. (2012). PMID: 23262285
    16. The modulation of DAF-2 protein level by nutritional status may constitute an important component in the irreversible commitment to dauer arrest. PMID: 22123849
    17. The daf-2 insulin-signaling mutant is able to reduce uterine masses at extreme age. PMID: 22562940
    18. Fluorodeoxyuridine affects the identification of metabolic responses to daf-2 status in Caenorhabditis elegans. PMID: 22116032
    19. The daf-2 pathway suppressed vitellogenesis through transcriptional mechanisms reflecting reproductive phenotypes, as well as distinct posttranscriptional mechanisms. PMID: 21749693
    20. Data show that glucose stress, oga-1 mutants, and ogt-1 mutants each result in an increase in daf-2 carbohydrate storage. PMID: 21441213
    21. Proteomic analyses of the environmentally resistant and 'nonaging' C. elegans dauer stage and long-lived daf-2 mutants has provided a unique insight into the protein changes which mediate survival against endogenously produced toxins. PMID: 20392130
    22. daf-2(e1370) and daf-2(e1370);pept-1(lg601) animals were compared with a focus on genes that showed significantly higher changes in expression levels in daf-2;pept-1 than in daf-2. PMID: 20550516
    23. These results suggest that polyunsaturated fatty acids (PUFA) act as mediators of daf-2/insulin signaling and that daf-16 might be involved in fatty acid homeostasis under the control of PUFA. PMID: 20226839
    24. Here we show that the C. elegans Insulin/IGF-1 receptor mutant daf-2 improves memory performance early in adulthood and maintains learning ability better with age but, surprisingly, demonstrates no extension in long-term memory with age. PMID: 20502519
    25. analysis of how divergent mechanisms control hypoxic sensitivity and lifespan by the DAF-2/insulin/IGF-receptor pathway PMID: 19936206
    26. daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the signaling pathway of this protein PMID: 12654727
    27. daf-2 mutants were resistant to killing by Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa PMID: 12817143
    28. Role of DAF-2 in longevity and aging, and interactions with DAF-16, are reviewed. PMID: 14516929
    29. findings show that reducing daf-2 mutant activity further by subjecting weak daf-2 mutants as young larvae to dal-2 RNA interference (RNAi) produced larger increases in life-span without triggering dauer formation PMID: 14576426
    30. The DAF-2 pathway happens in several organisms and mounted drug discovery programs around those targets. PMID: 15217602
    31. DAF-2 signaling controls mitochondrial bioenergetics by adjusting the rate of ATP synthesis to the rate of ATP utilization and by regulating the heat-producing proton leak pathway. PMID: 15708444
    32. Data show that an activated Ras mutation, let-60, weakly suppresses constitutive dauer diapause in daf-2 and age-1 (PI3K) mutants. PMID: 16164423
    33. REVIEW: role of DAF-2/insulin signaling on lifespan PMID: 16441841
    34. daf-2 has a role in conversion of fat to carbohydrate, and conservation of ATP stocks PMID: 16522328
    35. isolated dauer-formation mutations in C. briggsae, a species closely related to the model organism C. elegans and identified mutations in orthologs of C. elegans genes daf-2, daf-3 and daf-4 PMID: 17660533
    36. 86 proteins identified that were more or less abundant in long-lived daf-2 mutant worms; genetic studies on a subset of the proteins indicated they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage & aging PMID: 17673661
    37. Gene silencing dramatically shortens daf-2 mutant longevity but less dramatically shorten daf-2 life span. PMID: 18006689
    38. mutation of daf-12 marginally reduced life span in daf-2(+) hermaphrodites, as in plate-cultured populations, it did not in daf-2(+) males. PMID: 18056973
    39. consolidate and extend our understanding of the complex genetics of daf-2 and its underlying molecular biology in Caenorhabditis elegans. PMID: 18245374
    40. Hypoxia-mediated defects in nervous system development depended on signaling through the insulin-like receptor DAF-2, which serves to control the level of reactive oxygen species that also affects axon pathfinding. PMID: 18587389
    41. Pathogen resistance of daf-2, akt-1, and akt-2 mutants is associated with restricted bacterial colonization, and daf-2 mutants are better able to clear an infection after challenge with Pseudomonas aeruginosa. PMID: 18782349
    42. Pseudomonas aeruginosa suppresses host immunity by activating the DAF-2 insulin-like signaling pathway in Caenorhabditis elegans. PMID: 18927620

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 数据库链接：

    KEGG: cel:CELE_Y55D5A.5

    STRING: 6239.Y55D5A.5a

    UniGene: Cel.8775