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  4. Recombinant Human tRNA (guanine-N (7)-)-methyltransferase subunit WDR4 (WDR4)

Recombinant Human tRNA (guanine-N (7)-)-methyltransferase subunit WDR4 (WDR4)

  • 中文名称：
    人WDR4重组蛋白
  • 货号：
    CSB-YP026021HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人WDR4重组蛋白
  • 货号：
    CSB-EP026021HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人WDR4重组蛋白
  • 货号：
    CSB-EP026021HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人WDR4重组蛋白
  • 货号：
    CSB-BP026021HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人WDR4重组蛋白
  • 货号：
    CSB-MP026021HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WDR4
  • Uniprot No.：
  • 别名：
    OTTHUMP00000109401; OTTHUMP00000109402; TRM82; tRNA (guanine N(7) ) methyltransferase subunit WDR4; tRNA (guanine-N(7)-)-methyltransferase subunit WDR4; WD repeat containing protein 4; WD repeat protein 4; WD repeat protein domain 4 protein isoform 2; WD repeat-containing protein 4; wdr4; WDR4_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-412
  • 氨基酸序列
    AGSVGLALCGQTLVVRGGSRFLATSIASSDDDSLFIYDCSAAEKKSQENKGEDAPLDQGS GAILASTFSKSGSYFALTDDSKRLILFRTKPWQCLSVRTVARRCTALTFIASEEKVLVAD KSGDVYSFSVLEPHGCGRLELGHLSMLLDVAVSPDDRFILTADRDEKIRVSWAAAPHSIE SFCLGHTEFVSRISVVPTQPGLLLSSSGDGTLRLWEYRSGRQLHCCHLASLQELVDPQAP QKFAASRIAFWCQENCVALLCDGTPVVYIFQLDARRQQLVYRQQLAFQHQVWDVAFEETQ GLWVLQDCQEAPLVLYRPVGDQWQSVPESTVLKKVSGVLRGNWAMLEGSAGADASFSSLY KATFDNVTSYLKKKEERLQQQLEKKQRRRSPPPGPDGHAKKMRPGEATLSC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs. Also required for methylation of a specific subset of miRNAs, such as let-7. Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1.
  • 基因功能参考文献：
    1. WDR4 is an oncoprotein that negatively regulates PML via ubiquitination to promote lung cancer progression by fostering an immunosuppressive and prometastatic tumor microenvironment PMID: 28691927
    2. Combined autozygome/exome analysis revealed a novel missense mutation in WDR4 as the likely causal variant of Primordial dwarfism PMID: 26416026
    3. regulates FEN1's potential DNA cleavage threat near the site of replication PMID: 26751069
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    WD repeat TRM82 family
  • 数据库链接：

    HGNC: 12756

    OMIM: 605924

    KEGG: hsa:10785

    STRING: 9606.ENSP00000328671

    UniGene: Hs.248815