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  4. Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial

Recombinant Human parvovirus B19 Non-capsid protein NS-1 (NS1), partial

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  • 中文名称：
    Recombinant Human parvovirus B19 Non-capsid protein NS-1(NS1),partial
  • 货号：
    CSB-EP357083HPM
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP357083HPM could indicate that this peptide derived from E.coli-expressed Human parvovirus B19 (isolate AU) (HPV B19) NS1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP357083HPM could indicate that this peptide derived from E.coli-expressed Human parvovirus B19 (isolate AU) (HPV B19) NS1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NS1
  • Uniprot No.：
  • 别名：
    NS1; Initiator protein NS1; NS1; EC 3.1.21.-; EC 3.6.4.12; NCVP1; Non-capsid protein NS-1; Non-structural protein 1; Non-structural protein NS1
  • 种属：
    Human parvovirus B19 (isolate AU) (HPV B19)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    44.6kDa
  • 表达区域：
    1-255aa
  • 氨基酸序列
    MELFRGVLQVSSNVLDCANDNWWCSLLDLDTSDWEPLTHTNRLMAIYLSSVASKLDFTGGPLAGCLYFFQVECNKFEEGYHIHVVTGGPGLNPRNLTVCVEGLFNNVLYHLVTENVKLKFLPGMTTKGKYFRDGEQFIENYLMKKIPLNVVWCVTNIDGYIDTCISATFRRGACHAKKPRITTAINDTSSDAGESSGTGAEVVPFNGKGTKASIKFQTMVNWLCENRVFTEDKWKLVDFNQYTLLSSSHSGSFQI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this recombinant HPV B19 NS1 protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the NS1 protein. N-terminal 6xHis-SUMO tag was used in the process. The purity is 0.9 determined by SDS-PAGE.

    The non-structural protein 1 (NS1) of human parvovirus B19 plays a critical role in viral DNA replication, which performs many diferent functions during the virus life cycle. NS1 of parvovirus B19 induces cell death by apoptosis in at least erythroid-lineage cells by a pathway that involves caspase 3, whose activation may be a key event during NS1-induced cell death. Some studies showed that have shown that NS1 initiates apoptosis by activating caspase 3 (but not caspase 1) in a manner which is deferent from the IL-6 activation pathway. The cytotoxicity of NS1 in such cells results from chromosomal DNA damage caused by the DNA-nicking and DNA-attaching activities of NS1. Studies have been shown NS1 covalently binds to cellular DNA and is modiied by PARP (Poly ADP ribose polymerase), an enzyme involved in repairing single-stranded DNA nicks.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters. Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins. Upregulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition. This arrest promotes apoptosis for viral release.
  • 亚细胞定位：
    Host nucleus.
  • 蛋白家族：
    Parvoviruses non-capsid protein family