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  4. Recombinant Human immunodeficiency virus type 1 group M subtype B Virion infectivity factor (vif)

Recombinant Human immunodeficiency virus type 1 group M subtype B Virion infectivity factor (vif)

  • 货号：
    CSB-YP322701HKZ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP322701HKZ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP322701HKZ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP322701HKZ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP322701HKZ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    vif
  • Uniprot No.：
  • 别名：
    vifVirion infectivity factor; Vif; SOR protein) [Cleaved into: p17; p7]
  • 种属：
    Human immunodeficiency virus type 1 group M subtype B (isolate OYI) (HIV-1)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-192
  • 氨基酸序列
    MENRWQVMIV WQVDRMRIRT WKSLVKHHMY VSKKAKGWFY RHHYESTHPR ISSEVHIPLG DATLVVTTYW GLHTGEREWH LGQGASIEWR KKRYSTQVDP GLADQLIHTY YFDCFSESAI RNAILGNIVS PRCEYPAGHN KVGSLQYLAL AALIKPKKIK PPLPSVTKLT EDRWNKPQKT KGHRGSHTMN GH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Counteracts the innate antiviral activity of host APOBEC3F and APOBEC3G. Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions. Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F. In turn, they are directed to the 26S proteasome for degradation. Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. May interact directly with APOBEC3G mRNA in order to inhibit its translation. Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core. Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.
  • 亚细胞定位：
    Host cytoplasm. Host cell membrane; Peripheral membrane protein; Cytoplasmic side. Virion.
  • 蛋白家族：
    Primate lentivirus group Vif protein family