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  4. Recombinant Human Zinc finger protein Gfi-1 (GFI1)

Recombinant Human Zinc finger protein Gfi-1 (GFI1)

  • 中文名称：
    人GFI1重组蛋白
  • 货号：
    CSB-YP858722HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GFI1重组蛋白
  • 货号：
    CSB-EP858722HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GFI1重组蛋白
  • 货号：
    CSB-EP858722HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GFI1重组蛋白
  • 货号：
    CSB-BP858722HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GFI1重组蛋白
  • 货号：
    CSB-MP858722HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GFI1
  • Uniprot No.：
  • 别名：
    GFI 1; GFI1; GFI1_HUMAN; Growth factor independence 1; Growth factor independent 1; Growth factor independent protein 1; SCN2; Zinc finger protein 163; Zinc finger protein Gfi 1; Zinc finger protein Gfi-1; Zinc finger protein Gfi1; ZNF 163; ZNF163
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ LTEAPDRASA SPDSCEGSVC ERSSEFEDFW RPPSPSASPA SEKSMCPSLD EAQPFPLPFK PYSWSGLAGS DLRHLVQSYR PCGALERGAG LGLFCEPAPE PGHPAALYGP KRAAGGAGAG APGSCSAGAG ATAGPGLGLY GDFGSAAAGL YERPTAAAGL LYPERGHGLH ADKGAGVKVE SELLCTRLLL GGGSYKCIKC SKVFSTPHGL EVHVRRSHSG TRPFACEMCG KTFGHAVSLE QHKAVHSQER SFDCKICGKS FKRSSTLSTH LLIHSDTRPY PCQYCGKRFH QKSDMKKHTF IHTGEKPHKC QVCGKAFSQS SNLITHSRKH TGFKPFGCDL CGKGFQRKVD LRRHRETQHG LK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.
  • 基因功能参考文献：
    1. GFI1 facilitates efficient DNA repair by regulating PRMT1 dependent methylation of MRE11 and 53BP1. PMID: 29651020
    2. High GFI1 expression is associated with Acute myeloid leukemia. PMID: 28574132
    3. Differential methylation was observed in the CYP1A1 promoter and AHRR gene body regions between women who smoked throughout pregnancy and non-smokers on the fetal-side of the placenta and in the GFI1 promoter between women who quit smoking while pregnant and non-smokers on the maternal-side of the placenta PMID: 28820654
    4. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. PMID: 28387757
    5. Simvastatin attenuates the tumor-associated macrophage mediated gemcitabine resistance of PDAC by blocking the TGF-beta1/Gfi-1 axis. PMID: 27840243
    6. combination of the sequence-specific and nonspecific DNA-binding modes of SATB1 should be advantageous in a search for target loci during transcriptional regulation PMID: 27480105
    7. GFI1(36S) homozygous patients exhibited a sustained response to treatment with hypomethylating agents, whereas GFI1(36N) patients had a poor sustained response to this therapy. Because allele status of GFI1(36N) is readily determined using basic molecular techniques, we propose inclusion of GFI1(36N) status in future prospective studies for MDS patients to better predict prognosis and guide therapeutic decisions. PMID: 27080012
    8. Maternal-smoking sensitive CpG sites in newborns were significantly associated with cg18146737 SNP located proximal to GFI1. PMID: 27403598
    9. In this study, the influence of prenatal smoking exposure on the childrens' DNA methylation state of a CpG island located upstream of the promoter of the growth factor independent 1 (GFI1) gene was analyzed. Significant hypomethylation was observed in this CpG island in SIDS cases with cigarette smoke exposure compared to non-exposed cases. PMID: 27677632
    10. Gfi1 gene expression is regulated by cytokines in activated T cells. PMID: 27143377
    11. miR-495 directly interacts with the Gfi1 3'UTR to regulate Gfi1 at a post-transcriptional level and the expression level of miR-495 is inversely correlated with the Gfi1 protein level in medulloblastoma specimens. PMID: 26160036
    12. Gfi1 acts as a transcriptional repressor by recruiting histone-modifying enzymes to promoters and enhancers of target genes. Mutations of the C-terminal zinc finger domain causes congenital neutropenia. It may be involved in leukemia and lymphoma. Review. PMID: 26447191
    13. During infection, the incoming HCMV rapidly downregulates the GFI1 mRNA and protein in both wild-type cells and in cells in which EZH2, NDY1/KDM2B or JARID2 were knocked down. PMID: 24830456
    14. Data shows that MYB regulates the expression of endogenous human GFI1. PMID: 24121275
    15. results, supported by evidence from mouse models, identify GFI1 and GFI1B as prominent medulloblastoma oncogenes and implicate 'enhancer hijacking' as an efficient mechanism driving oncogene activation in a childhood cancer PMID: 25043047
    16. Lymphoid progenitor cells from childhood acute lymphoblastic leukemia are functionally deficient and express high levels of the transcriptional repressor Gfi-1. PMID: 24198842
    17. GFI1 is repressed by p53 PMID: 24023884
    18. the expression of Gfi-1 contributes to SOCS1 silencing in AML cells through epigenetic modification, and suppression of histone methyltransferase can provide new insight in AML therapy. PMID: 24018353
    19. Studies indicate that Gfi1 recruits LSD1 to p53 and dampens its activity by de-methylating p53 at C-terminal lysines to prevent immediate apoptosis. PMID: 23528269
    20. The competition for this binding site inhibits the expression of HOXA9 and induces different transcriptional outcomes, which suggests a new direction for investigation of the mechanism underlying targeted therapy of malignant gliomas. PMID: 23466061
    21. Our data suggest that the presence of a GFI136N variant allele induces a preleukemic state in myeloid precursors by deregulating the expression of Hoxa9 and other AML-related genes. PMID: 22932805
    22. Data suggest that Gfi1 may have an important role in prolonged multiple myeloma (MM)-induced osteoblast (OB) suppression and provide a new therapeutic target for MM bone disease. PMID: 22042697
    23. GFI1 may play an important role in leukemia, especially in CML incidence and progression. PMID: 20723283
    24. GFI-1 expression is upregulated in leukemia patients and may contribute to leukemogenesis. PMID: 20723286
    25. Studies indicate that Gfi1 is central to several transcriptional circuits whose dysregulation leads to abnormal or malignant hematopoiesis. PMID: 20571393
    26. An analysis and fine mapping of GFI-EVI5-RPL5-FAM69A locus, genotyping eight Tag-single nucleotide polymorphisms in 732 multiple sclerosis patients and 974 controls from Spain, was performed. PMID: 20087403
    27. a variant allele of GFI1 (GFI1(36N)) is associated with acute myeloid leukemia (AML) in white subjects PMID: 20075157
    28. Mutations in proto-oncogene GFI1 cause human neutropenia and target ELA2 PMID: 12778173
    29. role for auto- and trans-regulation of Gfi1 by GFI1 and GFI1B in maintaining the normal expression patterns of Gfi1 PMID: 15131254
    30. GFI1 in prostate cancer cells acts as a repressor of 25-hydroxyvitamin D 1-alpha hydroxylase gene. PMID: 15947108
    31. findings highlight the role of epigenetics in the regulation of development and oncogenesis by Gfi1 PMID: 16287849
    32. Gfi1 levels are regulated by the ubiquitin-proteasome system. It binds the promoters of several granulocyte-specific genes in primary monocytes. This correlated with low expression of these genes in monocytes compared with granulocytes. PMID: 16888099
    33. GFI1 may play a significant role in the down regulation of endogenous production of 1,25D in prostate cancer cells and could provide a novel insight to future diagnosis and treatment PMID: 17207994
    34. Decreased Gfi-1 levels in our SGD patient, together with the mutant C/EBPepsilon, block secondary granules proteins expression, thereby contributing to the underlying etiology of the disease in our patient. PMID: 17244686
    35. Results identify PRDM5, which acts as a sequence-specific, DNA binding transcription factor that targets hematopoiesis-associated protein-coding and microRNA genes, some of which are targets of Gfi1. PMID: 17636019
    36. The fine-tuning of Gfi1 protein levels regulated by Triad1 defines an unexpected role for this protein in hematopoiesis. PMID: 17646546
    37. The measurement of CD34 and GFI1 mRNA expressions could be useful as a diagnostic and prognostic marker for myelodysplastic syndrome. PMID: 18371060
    38. Ajuba is utilized as a corepressor selectively on Gfi1 target genes PMID: 18805794
    39. These results reveal novel contributions of MTGR1 and GFI1 to the regulation of neurite outgrowth and identify novel repressors of integrin-dependent neurite outgrowth. PMID: 19026687
    40. data reveal a mechanism of transcriptional repression by Gfi-1 and may have important implications for understanding the roles of Gfi-1 in normal development and tumorigenesis PMID: 19164764
    41. Data suggest that expression of PFAAP5 allows neutrophil elastase to potentiate the repression of Gfi1 target genes. PMID: 19506020
    42. Gfi1 mRNA level in both the mononuclear cells and purified CD34(+) cells from CML were significantly higher as measured by quantitative real-time PCR. PMID: 19887785

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  • 相关疾病：
    Neutropenia, severe congenital 2, autosomal dominant (SCN2); Dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA)
  • 亚细胞定位：
    Nucleus. Note=Colocalizes with PIAS3 and RUNX1T1 in nuclear dots.
  • 数据库链接：

    HGNC: 4237

    OMIM: 600871

    KEGG: hsa:2672

    STRING: 9606.ENSP00000294702

    UniGene: Hs.608370