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  4. Recombinant Human WD repeat-containing protein 5 (WDR5)

Recombinant Human WD repeat-containing protein 5 (WDR5)

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  • 中文名称：
    人WDR5重组蛋白
  • 货号：
    CSB-EP026038HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    WDR5
  • Uniprot No.：
  • 别名：
    2410008O07Rik; AA408785; AA960360; BIG 3; Big; BIG3; BMP2 induced 3 kb gene protein; BMP2 induced gene; 3-KB; BMP2-induced 3-kb gene protein; MGC114572; OTTHUMP00000162494; SWD 3; SWD3; SWD3 Set1c WD40 repeat protein homolog; WD repeat containing protein 5; WD repeat domain 5; WD repeat protein 5; WD repeat-containing protein 5; WD repeat-containing protein BIG-3; WDR 5; Wdr5; WDR5_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    52.5kDa
  • 表达区域：
    2-334aa
  • 氨基酸序列
    ATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B.
  • 基因功能参考文献：
    1. WDR5 was identified as a substrate of SETD6 and it was revealed that the methylation of specific lysines (K207/K325) of WDR5 was critical for maintaining global histone H3K4me3 levels and promoting breast cancer cell proliferation and migration PMID: 30226578
    2. WDR5 is aberrantly overexpressed in head neck squamouos cell carcinoma and associates with aggressiveness and unfavorable prognosis, thus representing a novel diagnostic and prognostic biomarker for the cancer. PMID: 29569374
    3. This is the first demonstration of the mechanism of E2/ERalpha signalling activation via the BIG3-PKA-PP1Calpha tri-complex in breast cancer cells. PMID: 28555617
    4. Data found that the expression of WDR5 increased in a majority of the human gastric cancer tissues. Its knockdown significantly inhibited the ability of cell proliferation, reversed cell cycle arrest as well as cell's tumorigenicity. Data reveal that WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in gastric cancer. PMID: 29125890
    5. findings reveal a nongenomic function for WDR5 in regulating H3K4 methylation induced by 3D environments, physical properties of the nucleus, cell polarity, and cell migratory capacity. PMID: 29987046
    6. HCMV infection increases protein levels of WD repeat-containing protein 5 (WDR5) during infection, overexpression of WDR5 enhances viral replication, and knockdown of WDR5 dramatically attenuates viral replication. The results indicate that WDR5 promotes the nuclear egress of viral capsids, the depletion of WDR5 resulting in a significant decrease in production of infectious virions. PMID: 29437978
    7. our findings indicate that HOXD-AS1 promotes proliferation, castration resistance, and chemo-resistance in prostate cancer by recruiting WDR5. PMID: 28487115
    8. Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation. PMID: 28813667
    9. The decrease in WDR5 expression was particularly evident in HD-hiPSCs compared to hESCs and control-hiPSCs from healthy subjects PMID: 28476540
    10. we demonstrate for the first time that targeted expression of miR-31-5p using a nonviral minicircle vector can serve as a novel approach for tumor miRNA therapy. Moreover, WDR5 may be a promising therapeutic target for NPC treatment. PMID: 28042945
    11. WDR5 may have oncogenic effect and WDR5-mediated H3K4 methylation plays an important role in leukemogenesis PMID: 27192115
    12. WDR5 functions to sustain proper execution of DNA replication in pancreatic ductal adenocarcinoma cells, as previously suggested by replication stress studies involving MLL1, and c-Myc, also found to interact with WDR5. PMID: 27320920
    13. This report documents a novel lncRNA, GClnc1, which may act as a scaffold to recruit the WDR5 and KAT2A complex and modify the transcription of target genes. This study reveals that GClnc1 is an oncogenic lncRNA in human gastric cancer. PMID: 27147598
    14. different cancer mutations in MLL1 lead to a loss or increase in activity, illustrating the complex and tumor-specific role of MLL1 in carcinogenesis. PMID: 28182322
    15. WDR5 shows a direct binding to the ZNF407 promoter. PMID: 28300833
    16. Taken together, these data suggest that WDR5 is directly involved in p53 signaling pathway. Our studies provide a new insight into WDR5 functions in A549 cells. PMID: 28412363
    17. Targeted Disruption of the Interaction between WD-40 Repeat Protein 5 (WDR5) and Mixed Lineage Leukemia (MLL)/SET1 Family Proteins Specifically Inhibits MLL1 and SETd1A Methyltransferase Complexes. PMID: 27563068
    18. WDR5 over-expression is associated with poor breast cancer clinical outcome. PMID: 26355959
    19. Results identify WDR5 as a key cofactor for N-Myc-regulated transcriptional activation and tumorigenesis and as a novel therapeutic target for MYCN-amplified neuroblastomas. PMID: 26471359
    20. BIG3 may block the KPNAs (KPNA1, KPNA5, and KPNA6) binding region(s) of PHB2. PMID: 26052702
    21. solution structures of the MLL3 core complex assembled with and without WDR5 by small angle x-ray scattering show similar overall topologies PMID: 26324722
    22. Target gene recognition by MYC depends on its interaction with WDR5. This interaction could create an avidity-based chromatin recognition mechanism allowing MYC to select its target genes in response to both genetic and epigenetic determinants. Review. PMID: 26383167
    23. we have discovered that WDR5 plays an important role in bladder cancer suggesting that WDR5 is a potential biomarker and a promising target in the treatment of bladder cancer. PMID: 25656485
    24. Wdr5-MLL interaction in C/EBPA N-terminal leukemia is a promising pharmacological target. PMID: 26167872
    25. findings prove that the TSP-1/CD47/SIRP-alpha signal axis is important to the evolution of tumor cells in the microenvironment of immunotherapy and identify thrombospondin-1 as a key signal PMID: 25666610
    26. results identify WDR5 as a critical epigenomic integrator of histone phosphorylation and methylation and as a major driver of androgen-dependent prostate cancer cell proliferation PMID: 24793694
    27. BIG3(WRD5)-PHB2 interaction is critical for the tamoxifen resistance of breast cancer cells; its targeting reverses the resistance. PMID: 24051437
    28. Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID: 24235145
    29. data are consistent with a model in which WDR5 binds the gamma-globin promoter in a PRMT5-dependent manner. PMID: 22689669
    30. A small molecule antagonist binds to WDR5 in the peptide-binding pocket which inhibits the catalytic activity of mixed-lineage leukemia protein. PMID: 22989411
    31. we defined a role for Trithorax proteins WDR5 and MLL2 in activating the differentiation gene program in epidermal progenitor cells PMID: 22829784
    32. WDR5-Win motif interaction is important for the assembly of the MLL1 core complex in vivo. PMID: 22665483
    33. findings show that WDR5 is a direct target of SRY; the interaction of WDR5 and SRY activates Sox9 expression; results suggest that, in conjunction with SRY, WDR5 plays an important role in sex determination PMID: 22523547
    34. WDR5 as a critical substrate of CUL4B in regulating neuronal gene expression. PMID: 21816345
    35. crystal structure of WDR5 in ternary complex with RbBP5 and MLL1 PMID: 21220120
    36. the change of folding free energy by mutations mainly corresponds to the deletion of hydrogen bonds. PMID: 20939513
    37. NSL is composed of nine subunits. Two of its subunits, WD repeat domain 5 (WDR5) and host cell factor 1 (HCF1), are shared with members of the MLL/SET family of histone H3 lysine 4 (H3K4) methyltransferase complexes. PMID: 20018852
    38. Results show that Depletion of CHD8 enhances HOXA2 expression and a loss of the WDR5/Ash2L/RbBP5 subcomplex. PMID: 20085832
    39. WDR5 is essential in assembling a virus-induced VISA-associated complex and plays an important role in virus-triggered induction of type I interferons. PMID: 20080758
    40. The WDR5 is required for binding of the methyltransferase complex to the K4-dimethylated H3 tail as well as for global H3 K4 trimethylation and HOX gene activation in human cells. PMID: 15960974
    41. Wdr5 has a funcitonal role in endocondral bone formation PMID: 16340128
    42. the high-resolution X-ray structures of WDR5 in the unliganded form and complexed with histone H3 peptides having unmodified and mono-, di- and trimethylated K4 PMID: 16829959
    43. the crystal structure of WDR5 bound to a histone H3 PMID: 16829960
    44. WDR5 mediates interactions of the MLL1 catalytic unit both with the common structural platform and with the histone substrate. PMID: 16878130
    45. involvement of WDR5 in binding and presenting histone H3K4 for further methylation PMID: 16946699
    46. WDR5's recognition of arginine 3765 of MLL1 is essential for the assembly and enzymatic activity of the MLL1 core complex in vitro PMID: 18829457
    47. WDR5 recognizes Arg-3765 of MLL1, which is essential for the assembly and enzymatic activity of the MLL1 core complex PMID: 18829459
    48. analysis of interactions between WDR5, the catalytic subunit, MLL, and the substrate, histone H3, of the MLL complex PMID: 18840606
    49. The identified components revealed factors involved in histone methylation and cell cycle control and include Ash2L, RbBP5, WDR5, HCF-1, DBC-1, and EMSY. PMID: 19131338
    50. Activation of an estrogen/estrogen receptor signaling by BIG3 through its inhibitory effect on nuclear transport of PHB2/REA in breast cancer is reported. PMID: 19496786

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat WDR5/wds family
  • 数据库链接：

    HGNC: 12757

    OMIM: 609012

    KEGG: hsa:11091

    STRING: 9606.ENSP00000351446

    UniGene: Hs.397638