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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 E1 (UBE2E1)

Recombinant Human Ubiquitin-conjugating enzyme E2 E1 (UBE2E1)

  • 中文名称：
    人UBE2E1重组蛋白
  • 货号：
    CSB-YP025448HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人UBE2E1重组蛋白
  • 货号：
    CSB-EP025448HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人UBE2E1重组蛋白
  • 货号：
    CSB-EP025448HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人UBE2E1重组蛋白
  • 货号：
    CSB-BP025448HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人UBE2E1重组蛋白
  • 货号：
    CSB-MP025448HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    UB2E1_HUMAN; UbcH6; Ube2e1; Ubiquitin carrier protein E1; Ubiquitin conjugating enzyme E2E 1 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 1; Ubiquitin-conjugating enzyme E2 E1; ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-193
  • 氨基酸序列
    SDDDSRAST SSSSSSSSNQ QTEKETNTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH DRMARQWTKR YAT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
  • 基因功能参考文献：
    1. UBE2E1 expression was adversely correlated with AML survival; UBE2E1 expression was negatively correlated with patients' response to induction chemotherapy PMID: 27855695
    2. IPO11 provides protection in another way, the researchers found. The transport receptor also shuttles UBE2E1, an enzyme necessary for PTEN ubiquitination, into the nucleus. Thanks to IPO11, normal cells keep all of their UBE2E1 in the nucleus, allowing PTEN to continue functioning in the cytoplasm. In cells lacking IPO11, UBE2E1 remains in the cytoplasm and promotes the destruction of PTEN. PMID: 28258103
    3. study reveals that UbE2E1 is an in vivo E2 for the PRC1 ligase complex and thus plays an important role in the regulation of H2A Lys-119 monoubiquitination PMID: 28073915
    4. Auto-ubiquitination of Ube2E1 is intramolecular.RNF4 enhances Ube2E1 self-ubiquitination. PMID: 25960396
    5. USP7 is critical in maintaining the steady state levels of UbE2E1 in cells. PMID: 23603909
    6. isolated ISG15-modified and unmodified UbcH6 proteins, and analyzed their abilities to form thioester intermediates with ubiquitin PMID: 16428300
    7. UbcH6 and ataxin-1 are E2-substrate cognate pairs in the ubiquitin-proteasome system. PMID: 18439907
    8. UbcH6 modulates the transcriptional repression activity of ataxin-1 by modulating the degradation of ataxin-1. PMID: 18519031
    9. E1 ligase of the proteasome is inhibitd by ziram and has a role in preventing dopaminergic cell damage PMID: 18818210
    10. Smallangle X-ray scattering experiments show that the Lys63 tetra-Ub chain is dynamic in solution, adopting an ensemble of conformations that are more compact than the extended form in the crystal. PMID: 19664638

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    HGNC: 12477

    OMIM: 602916

    KEGG: hsa:7324

    STRING: 9606.ENSP00000303709

    UniGene: Hs.164853