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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 A (UBE2A)

Recombinant Human Ubiquitin-conjugating enzyme E2 A (UBE2A)

  • 中文名称：
    人UBE2A重组蛋白
  • 货号：
    CSB-YP025438HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人UBE2A重组蛋白
  • 货号：
    CSB-EP025438HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人UBE2A重组蛋白
  • 货号：
    CSB-EP025438HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人UBE2A重组蛋白
  • 货号：
    CSB-BP025438HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人UBE2A重组蛋白
  • 货号：
    CSB-MP025438HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BHR6A; hHR6A; HR6A; mHR6A; MRXS30; MRXSN; RAD6 homolog A; RAD6A; UBC2; UBCD6; UBE2A; Ube2a ubiquitin-conjugating enzyme E2A; UBE2A_HUMAN; Ubiquitin carrier protein A; Ubiquitin carrier protein; Ubiquitin conjugating enzyme E2 17 kDa; Ubiquitin conjugating enzyme E2 21.5 kDa; Ubiquitin conjugating enzyme E2A (RAD6 homolog); Ubiquitin conjugating enzyme E2A; Ubiquitin protein ligase A; Ubiquitin-conjugating enzyme E2 A; Ubiquitin-protein ligase A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-152
  • 氨基酸序列
    MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
  • 基因功能参考文献：
    1. RAD6 is upregulated in response to chemotherapy and significantly correlated with expression of ovarian cancer (OC) stem cell signaling genes ALDH1A1 and SOX2 and poor prognosis of OC patients. PMID: 28806395
    2. RAD6 promotes proteasome activity and nuclear translocation by enhancing the degradation of PSMF1 and the lamin B receptor. PMID: 28031328
    3. Data show that the ubiquitin-conjugating enzyme E2 RAD6A/B-MDM2 ubiquitin ligase machinery regulates anti-silencing function 1A protein (ASF1A) degradation. PMID: 26336826
    4. Results showed KCMF1 C-terminus binds directly to RAD6, whereas N-terminal domains interact with UBR4 and point mutations found in X-linked intellectual disability (XLID) patients specifically lose the interaction with KCMF1 and UBR4. PMID: 25582440
    5. This study investigates clinical and molecular data of two unrelated, affected males with chromosome Xq24 deletions encompassing UBE2A. PMID: 25287747
    6. RAD6 physically interacts with heterochromatin protein 1alpha and ubiquitinates HP1alpha at residue K154, thereby promoting heterochromatin protein 1alpha degradation through the autophagy pathway PMID: 25384975
    7. HHR6 and hRad18 can monoubiquitinate FANCD2 at lysine 561 in vitro. This activity may represent a novel stress response pathway. PMID: 24036990
    8. RNF168, in complex with RAD6A or RAD6B, is activated in the DNA-damage-induced protein ubiquitination cascade. PMID: 23525009
    9. RAD6A is a regulator of Parkin-dependent mitophagy plays a critical role in maintaining neuronal function. PMID: 23685073
    10. UBE2A specifically interacts with CDK9, but not CDK2 and is phosphorylated by CDK9 in vitro. PMID: 22592529
    11. RAD6 can form a ternary complex with MDM2 and p53 that contributes to the degradation of p53. PMID: 22083959
    12. UBE2A deficiency syndrome is reported in two male patients. PMID: 21108393
    13. showed that the function of FA signaling pathway is at least partly mediated through coupling with hRad6/hRad18 signaling (HHR6 pathway) PMID: 20967207
    14. Novel missense mutations in the ubiquitination-related gene UBE2A cause a recognizable X-linked mental retardation syndrome PMID: 20412111
    15. high resolution backbone structure from nmr PMID: 11885984
    16. A single-nucleotide substitution, c.382C-->T in UBE2A, led to a premature UAG stop codon (Q128X). This is the first description of a mutation in a ubiquitin-conjugating enzyme gene as the cause of a human disease. PMID: 16909393

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  • 相关疾病：
    Mental retardation, X-linked, syndromic, Nascimento-type (MRXSN)
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    HGNC: 12472

    OMIM: 300860

    KEGG: hsa:7319

    STRING: 9606.ENSP00000360613

    UniGene: Hs.379466