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  4. Recombinant Human Tyrosyl-DNA phosphodiesterase 2 (TDP2)

Recombinant Human Tyrosyl-DNA phosphodiesterase 2 (TDP2)

  • 中文名称：
    人TDP2重组蛋白
  • 货号：
    CSB-YP528514HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TDP2重组蛋白
  • 货号：
    CSB-EP528514HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TDP2重组蛋白
  • 货号：
    CSB-EP528514HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TDP2重组蛋白
  • 货号：
    CSB-BP528514HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TDP2重组蛋白
  • 货号：
    CSB-MP528514HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TDP2
  • Uniprot No.：
  • 别名：
    5'-Tyr-DNA phosphodiesterase; 5'-tyrosyl-DNA phosphodiesterase; AD 022; AD022; dJ30M3.3; EAP 2; EAP II; EAP2; EAPII; ETS 1 associated protein 2; ETS 1 associated protein II; ETS1 associated protein 2; ETS1 associated protein II; ETS1-associated protein 2; ETS1-associated protein II; MGC111021; MGC9099; RP1-30M3.3; tdp2; TRAF and TNF receptor associated protein; TRAF and TNF receptor-associated protein; TTRAP; TYDP2_HUMAN; Tyr DNA phosphodiesterase 2; Tyr-DNA phosphodiesterase 2; Tyrosyl DNA phosphodiesterase 2; Tyrosyl-DNA phosphodiesterase 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    MELGSCLEGG REAAEEEGEP EVKKRRLLCV EFASVASCDA AVAQCFLAEN DWEMERALNS YFEPPVEESA LERRPETISE PKTYVDLTNE ETTDSTTSKI SPSEDTQQEN GSMFSLITWN IDGLDLNNLS ERARGVCSYL ALYSPDVIFL QEVIPPYYSY LKKRSSNYEI ITGHEEGYFT AIMLKKSRVK LKSQEIIPFP STKMMRNLLC VHVNVSGNEL CLMTSHLEST RGHAAERMNQ LKMVLKKMQE APESATVIFA GDTNLRDREV TRCGGLPNNI VDVWEFLGKP KHCQYTWDTQ MNSNLGITAA CKLRFDRIFF RAAAEEGHII PRSLDLLGLE KLDCGRFPSD HWGLLCNLDI IL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.; (Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.
  • 基因功能参考文献：
    1. Data indicate that tyrosyl-DNA phosphodiesterase 2 (TDP2) alone does not remove DNA topoisomerase II (TOP2)-DNA complexes from genomic DNA in vitro and that depletion of TDP2 in cells does not slow the removal of TOP2-DNA complexes. PMID: 30011940
    2. TDP2 re-localizes from the nucleus to the cytoplasm of EMCV infected cells. PMID: 29353210
    3. TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription. PMID: 28794467
    4. Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions. PMID: 27814490
    5. Study revealed a post-translational regulation of TDP2 activity and discovered a new role of ERK3 in increasing cancer cells' DNA damage response and chemoresistance to Top2 inhibitors. PMID: 26701725
    6. Data show that DNA-repair enzyme TDP2 knockdown in HepG2 cells significantly delays the conversion of relaxed circular DNA (RC-DNA) to covalently closed circular (ccc) DNA. PMID: 25201958
    7. This article summarizes and compares the biochemistry, functions, and post-translational regulation of TDP1 and TDP2, as well as the relevance of TDP1 and TDP2 as determinants of response to anticancer agents PMID: 24856239
    8. Proteolytic degradation of Top2 enables the processing of Top2.DNA and Top2.RNA covalent complexes by TDP2. PMID: 24808172
    9. TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function. PMID: 24658003
    10. Mutations in the 5'-tyrosyl-DNA phosphodiesterase activity of TTRAP,in particular TTRAP(E152A), showed decreased inhibitory activity on cell growth. PMID: 23433115
    11. Magnesium is indispensable for a 5'-tyrosyl DNA phosphodiesterase activity. PMID: 22405347
    12. VPg unlinkase activity in different mammalian cell lines correlates with their differential expression of TDP2. PMID: 22908287
    13. Biochemical characterization of human tyrosyl-DNA phosphodiesterase 2 (TDP2/TTRAP): a Mg(2+)/Mn(2+)-dependent phosphodiesterase specific for the repair of topoisomerase cleavage complexes. PMID: 22822062
    14. findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress. PMID: 21921940
    15. both FOXP2 and KIAA0319/TTRAP/THEM2 genes play an important role in human language development, but probably through different cerebral pathways. PMID: 22262880
    16. EAPII is an oncogenic factor and the activation of MAPK-ERK signaling pathway by EAPII may contribute to lung cancer development. PMID: 21478903
    17. an important mechanism for resistance to Top2-induced chromosome breakage and the possibility that TDP2 is a significant factor in cancer development and treatment PMID: 21030584
    18. PhiC31 integrase interacts with TTRAP and inhibits the NFkappaB activation. PMID: 19757154
    19. interacts with ETS1 and modulates its transcriptional function PMID: 12743594
    20. a significant association with (TTRAP)on reading and spelling ability was observed PMID: 17597587
    21. TTRAP is a novel PML nuclear body protein. PMID: 18706885
    22. This is the first time that TTRAP has been shown to interact with HIV-1 integrase and facilitate lentiviral vector integration. PMID: 19580783
    23. TTRAP is the first human 5'-tyrosyl DNA phosphodiesterase to be identified, and we suggest that this enzyme is denoted tyrosyl DNA phosphodiesterase-2 (TDP2). PMID: 19794497

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  • 相关疾病：
    Spinocerebellar ataxia, autosomal recessive, 23 (SCAR23)
  • 亚细胞定位：
    Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm.; Cytoplasm.
  • 蛋白家族：
    CCR4/nocturin family
  • 组织特异性：
    Widely expressed. Highly expressed in various brain regions, including the frontal and occipital lobes, the hippocampus, the striatum and the cerebellum.
  • 数据库链接：

    HGNC: 17768

    OMIM: 605764

    KEGG: hsa:51567

    STRING: 9606.ENSP00000367440

    UniGene: Hs.403010