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  4. Recombinant Human Transmembrane prolyl 4-hydroxylase (P4HTM), partial

Recombinant Human Transmembrane prolyl 4-hydroxylase (P4HTM), partial

  • 中文名称：
    人P4HTM重组蛋白
  • 货号：
    CSB-YP878913HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人P4HTM重组蛋白
  • 货号：
    CSB-EP878913HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人P4HTM重组蛋白
  • 货号：
    CSB-BP878913HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人P4HTM重组蛋白
  • 货号：
    CSB-MP878913HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    P4HTM
  • Uniprot No.：
  • 别名：
    EGLN4; HIF PH4; HIF Prolyl Hydroxylase 4; HIF prolyl hydroxylase PH 4; HIF prolyl hydroxylase PH4; HIF-PH4; HIF-prolyl hydroxylase 4; HIFPH4; HPH 4; HPH-4; Hypoxia inducible factor prolyl 4 hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD 4; Proline 4 hydroxylase; prolyl 4-hydroxylase ; Transmembrane prolyl 4-hydroxylase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • 基因功能参考文献：
    1. The results suggest that SESN2 increases degradation of HIF-1A via AMPK-PHD regulation that contributes to inhibition of in vitro and in vivo tumorigenesis. PMID: 27840318
    2. This review will expand our knowledge of biology of HIFs, PHDs, PHD inhibitors, and bone regeneration, and it may also aid the design of novel therapies for accelerating bone repair and regeneration or inhibiting bone tumours. [review] PMID: 24895555
    3. Results suggest that increased expression of prolyl hydroxylase (PH) might play an role in the physiology of uterine leiomyoma during the menstrual cycle. PMID: 23241241
    4. Data suggest that PH-4 is a novel hypoxia-inducible factor-prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. PMID: 12163023
    5. the longest and shortest isoenzymes have major transcripts encoding inactive polypeptides, which suggest novel regulation by alternative splicing. PMID: 12788921
    6. crystal structure of the peptide-substrate-binding domain PMID: 15456751
    7. This review summarizes recent progress in elucidating the molecular mechanisms of hypoxia-inducible factor (HIF)-1 activation, focusing on the role of oxygen-dependent asparaginyl hydroxylase in hypoxia signal transduction. PMID: 16154531

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein.
  • 组织特异性：
    Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
  • 数据库链接：

    HGNC: 28858

    OMIM: 614584

    KEGG: hsa:54681

    STRING: 9606.ENSP00000341422

    UniGene: Hs.654944