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  4. Recombinant Human Transcription initiation factor IIE subunit beta (GTF2E2)

Recombinant Human Transcription initiation factor IIE subunit beta (GTF2E2)

  • 中文名称：
    人GTF2E2重组蛋白
  • 货号：
    CSB-YP010003HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GTF2E2重组蛋白
  • 货号：
    CSB-EP010003HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GTF2E2重组蛋白
  • 货号：
    CSB-BP010003HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GTF2E2重组蛋白
  • 货号：
    CSB-MP010003HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GTF2E2
  • Uniprot No.：
  • 别名：
    FE; General transcription factor IIE subunit 2; General transcription factor IIE; polypeptide 2; beta 34kDa ; gtf2e2; T2EB_HUMAN; TF2E 2; TF2E2; TFIIE B; TFIIE-beta; Transcription initiation factor IIE beta subunit; Transcription initiation factor IIE subunit beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-291
  • 氨基酸序列
    MDPSLLRERE LFKKRALSTP VVEKRSASSE SSSSSSKKKK TKVEHGGSSG SKQNSDHSNG SFNLKALSGS SGYKFGVLAK IVNYMKTRHQ RGDTHPLTLD EILDETQHLD IGLKQKQWLM TEALVNNPKI EVIDGKYAFK PKYNVRDKKA LLRLLDQHDQ RGLGGILLED IEEALPNSQK AVKALGDQIL FVNRPDKKKI LFFNDKSCQF SVDEEFQKLW RSVTVDSMDE EKIEEYLKRQ GISSMQESGP KKVAPIQRRK KPASQKKRRF KTHNEHLAGV LKDYSDITSS K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 基因功能参考文献：
    1. functional analysis of Trichothiodystrophy causative TFIIEbeta mutation demonstrates that mutant TFIIEbeta strongly reduces the total amount of the entire TFIIE complex, with a remarkable temperature-sensitive transcription defect. PMID: 28973399
    2. We underline role of TFIIE in transcription and its distinction from nucleotide excision repair. Trichothiodystrophy individuals with mutations in GTF2E2 highlight the importance of the direct interaction between TFIIH and TFIIE in transcription process. PMID: 26996949
    3. PC4 plays essential roles in the transition step from transcription initiation to elongation by binding to melted DNA in collaboration with TFIIEbeta. PMID: 25308091
    4. introduced point mutations into two regions located near the carboxy terminus of TFIIE beta and identified the functionally essential amino acid residues that bind to RNA polymerase II PMID: 12665589
    5. TFIIE is an alpha/beta heterodimer in solution; A model for the quaternary architecture of the complex is proposed that provides a structural framework to discuss the function of TFIIE in the context of RNA polymerase II transcription initiation. PMID: 16547462
    6. These data suggest a model in which TFIIE binds to a position adjacent to the initiation site via the forkhead domain, enabling TFIIH to begin opening the promoter. PMID: 19210545

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  • 相关疾病：
    Trichothiodystrophy 6, non-photosensitive (TTD6)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIE beta subunit family
  • 数据库链接：

    HGNC: 4651

    OMIM: 189964

    KEGG: hsa:2961

    STRING: 9606.ENSP00000348168

    UniGene: Hs.77100