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  4. Recombinant Human Transcription factor MafA (MAFA)

Recombinant Human Transcription factor MafA (MAFA)

  • 中文名称：
    人MAFA重组蛋白
  • 货号：
    CSB-YP818821HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MAFA重组蛋白
  • 货号：
    CSB-EP818821HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人MAFA重组蛋白
  • 货号：
    CSB-EP818821HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MAFA重组蛋白
  • 货号：
    CSB-BP818821HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MAFA重组蛋白
  • 货号：
    CSB-MP818821HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAFA
  • Uniprot No.：
  • 别名：
    HMafA; Mafa; MAFA_HUMAN; Pancreatic beta cell specific transcriptional activator; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1; Transcription factor MafA; Transcription factor RIPE3b1; v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian); v maf musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-353
  • 氨基酸序列
    MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL SSTPLSTPCS SVPSSPSFCA PSPGTGGGGG AGGGGGSSQA GGAPGPPSGG PGAVGGTSGK PALEDLYWMS GYQHHLNPEA LNLTPEDAVE ALIGSGHHGA HHGAHHPAAA AAYEAFRGPG FAGGGGADDM GAGHHHGAHH AAHHHHAAHH HHHHHHHHGG AGHGGGAGHH VRLEERFSDD QLVSMSVREL NRQLRGFSKE EVIRLKQKRR TLKNRGYAQS CRFKRVQQRH ILESEKCQLQ SQVEQLKLEV GRLAKERDLY KEKYEKLAGR GGPGSAGGAG FPREPSPPQA GPGGAKGTAD FFL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.
  • 基因功能参考文献：
    1. We investigated a large pedigree with autosomal dominant inheritance of diabetes mellitus or insulinomatosis, an adult-onset condition of recurrent hyperinsulinemic hypoglycemia caused by multiple insulin-secreting neuroendocrine tumors of the pancreas. Using exome sequencing, we identified a missense MAFA mutation (p.Ser64Phe, c.191C>T) segregating with both phenotypes of insulinomatosis and diabetes. PMID: 29339498
    2. PDX1, Neurogenin-3, and MAFA are critical transcription regulators for beta cell development and regeneration. (Review) PMID: 29096722
    3. USP5 regulates c-Maf stability and multiple myeloma cell survival. PMID: 28933784
    4. Pdx1 and MafA play crucial roles in the pancreas and maintain mature beta-cell function. Our results showed that the expression of Pdx1 and MafA were significantly upregulated after a sleeve gastrectomy for morbid obesity. PMID: 26781599
    5. MAFA controls autonomic nervous system-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) receptor genes, which is impaired in patients with type 2 diabetes. PMID: 26904947
    6. These findings demonstrate that regulation of monoamine levels by Mao activity in beta cells is pivotal for physiological insulin secretion and that loss of MaoB expression may contribute to the beta cell dysfunction in type 2 diabetes. PMID: 26546820
    7. MAFA, MAFB, NKX6.1, and PDX1 activity provides a gauge of islet beta cell function, with loss of MAFA (and/or MAFB) representing an early indicator of beta cell inactivity PMID: 23863625
    8. Loss of MAFA expression is associated with insulinoma. PMID: 24157940
    9. MAFA nuclear expression in pancreatic alpha and beta cells, and the percentage of alpha cells expressing PAX4 are altered in patients with type 2 diabetes. PMID: 24013263
    10. under oxidative and nonoxidative conditions p38 MAPK directly binds to MafA and triggers MafA degradation via ubiquitin proteasomal pathway. PMID: 23660596
    11. Beta cell nuclear MafA is markedly decreased in humans with type 2 diabetes, which may contribute to impaired beta cell dysfunction. PMID: 22847061
    12. Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
    13. Data suggest that MafA plays a novel role in the reprogramming of stem cells into pancreatic beta-progenitors, promotes the islet-like characteristics of PDMSCs, as well as functionally regulation of blood glucose levels in transplanted grafts. PMID: 20158571
    14. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    15. MafA transcription is upregulated in beta-cells acutely cultured in high glucose similar to what may occur in vivo under normoglycemic conditions. PMID: 21278484
    16. a novel relationship between the phosphoamino acid-rich transactivation and b-Zip domains in controlling MafA DNA-binding activity. PMID: 20208071
    17. In addition to its expression in pancreatic beta cells, MafA also identifies the early ret-expressing sensory neurons in the dorsal root ganglia. PMID: 20213756
    18. gene is unlikely to have a significant role in monogenic diabetes in humans PMID: 19573128
    19. MafA selectively induces endogenous insulin transcription in non-beta cells PMID: 14973194
    20. mafA has a role in regulating insulin gene expression in the liver PMID: 15664997
    21. MafA plays a key role in coordinating and controlling the level of insulin gene expression in islet beta cells. PMID: 15665000
    22. Co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene. PMID: 15993959
    23. Data show that FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site. PMID: 16847327
    24. MafA has a critical role in islet beta-cell function PMID: 17636040
    25. Under diabetic conditions, expression and/or activities of PDX-1 and MafA in beta-cells are reduced [REVIEW]. PMID: 18508668
    26. Significance of large Maf proteins to Pdx1 expression in beta cells, and in particular MafB during pancreatic development. PMID: 18522939
    27. Data suggest that a group of transcription factors, including MafA and Foxa2, regulate expression of two major autoantigens in type 1 diabetes, including islet-specific glucose-6-phosphatase catalytic subunit-related protein. PMID: 18753309
    28. MafA levels are tightly controlled by the coordinated action of multiple kinase pathways PMID: 18948074
    29. MafA plays a crucial role in pancreatic beta-cells and could be a novel therapeutic target for diabetes PMID: 19393272
    30. p38 MAPK is a major regulator of MafA protein stability under oxidative stress PMID: 19407223
    31. Transcriptional and transforming activities of MafA are positively regulated by GSK-3-mediated phosphorylation. PMID: 18042454

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, Maf subfamily
  • 组织特异性：
    Expressed in the islets of Langerhans (at protein level).
  • 数据库链接：

    HGNC: 23145

    OMIM: 610303

    KEGG: hsa:389692

    STRING: 9606.ENSP00000328364

    UniGene: Hs.521914