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  4. Recombinant Human Transcription factor IIIB 50 kDa subunit (BRF2)

Recombinant Human Transcription factor IIIB 50 kDa subunit (BRF2)

  • 中文名称：
    Recombinant Human Transcription factor IIIB 50 kDa subunit(BRF2),Yeast
  • 货号：
    CSB-YP872533HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Transcription factor IIIB 50 kDa subunit(BRF2),Yeast
  • 货号：
    CSB-EP872533HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Transcription factor IIIB 50 kDa subunit(BRF2),Yeast
  • 货号：
    CSB-EP872533HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Transcription factor IIIB 50 kDa subunit(BRF2),Yeast
  • 货号：
    CSB-BP872533HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Transcription factor IIIB 50 kDa subunit(BRF2),Yeast
  • 货号：
    CSB-MP872533HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BRF2
  • Uniprot No.：
  • 别名：
    B-related factor 2; BRF-2; BRF2; BRF2_HUMAN; BRFU; FLJ11052; hBRFU; hTFIIIB50; RNA polymerase III transcription initiation factor BRF2; TFIIIB50; Transcription factor IIB- related factor TFIIIB50; Transcription factor IIIB 50 kDa subunit
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-419
  • 氨基酸序列
    MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA YRHSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGAICT LLYADLDVFS STYMQIVKLL GLDVPSLCLA ELVKTYCSSF KLFQASPSVP AKYVEDKEKM LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA DRLSCSLARF CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR PASPALLLPP CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR DFQRAQAARQ AATSVPNPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.
  • 基因功能参考文献：
    1. Results show that the mRNA and protein expression levels of BRF2 were markedly higher in non-small cell lung cancer (NSCLC) cells and highly related to differentiation degree, lymph node metastasis, clinical stage, and vascular invasion. miR-373 was identified to directly target BRF2, resulting in the enhanced migration and invasion of NSCLC A549 cells and indicating that BRF2 affects the metastasis of NSCLC. PMID: 29025258
    2. REVIEW: highlights the interplay of Brf2 with the Nrf2/Keap1 pathway, as well as the role of Brf2 in cancer and other possible regulations PMID: 28854119
    3. Data indicate the crystal structure of a Brf2-TBP-Bdp1 complex bound to a DNA promoter. PMID: 28743884
    4. Study solved crystal structures of a human Brf2-TATA-binding complex bound to natural promoters, obtaining a detailed view of the molecular interactions occurring at Brf2-dependent Pol III promoters and highlighting the general structural and functional conservation of human Pol II and Pol III pre-initiation complexes. PMID: 26638071
    5. hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
    6. These results suggested that BRF2 overexpression in tumor tissues is significantly associated with the poor prognosis of NSCLC patients through promoting epithelial-mesenchymal transition (EMT) program. PMID: 24738062
    7. BRF2 may have a role in poor prognosis and anti-angiogenic therapy for early-stage non-small cell lung cancer PMID: 24523874
    8. High expression of BRF2 is closely associated with tumor progression, angiogenesis, and poor survival in esophageal squamous cell cancer. PMID: 23550276
    9. BRF2 can serve as a marker for lung squamous cell carcinoma and may provide a novel target for therapy. PMID: 20668658
    10. C-terminal domain of Brf2 is required for efficient association of the protein with U6 promoter-bound TBP and SNAP(c), a type 3 promoter-specific transcription factor, and for efficient recruitment of Bdp1 PMID: 16227591
    11. deregulation of Brf1 and Brf2 expression could be a key mechanism responsible for the observed deregulation of RNA pol III transcription in cancer cells PMID: 18700021

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIB family
  • 数据库链接：

    HGNC: 17298

    OMIM: 607013

    KEGG: hsa:55290

    STRING: 9606.ENSP00000220659

    UniGene: Hs.709301