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  4. Recombinant Human Toll/interleukin-1 receptor domain-containing adapter protein (TIRAP)

Recombinant Human Toll/interleukin-1 receptor domain-containing adapter protein (TIRAP)

  • 货号：
    CSB-EP023570HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    TIRAP; MAL; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    50.9kDa
  • 表达区域：
    1-221aa
  • 氨基酸序列
    MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
  • 基因功能参考文献：
    1. TIRAP is phosphorylated at Thr28 within its phosphoinositide (PI)-binding motif (PBM), which leads to its ubiquitination and degradation. The s demonstrate that phosphorylation distorts the helical structure of TIRAP PBM, reducing PI interactions and cell membrane targeting. PMID: 28225045
    2. MAL is not disulfide-bonded and requires glutathionylation of C91 for signaling. PMID: 28739909
    3. The Mal-Toll/interleukin-1 receptor (TIR) domains AB loop is capable of mediating direct binding to the TIR domains of TLR4 and MyD88 simultaneously. PMID: 22485159
    4. MAL is multifunctional and integral for the molecular control of bacterial infections as well as inflammatory diseases. [Review] PMID: 23983209
    5. Data indicate that TcpB (Q8YF5, BtpA/Btp1) interacts with MAL, MyD88, and TLR4 but interferes only with the MAL-TLR4 interaction. PMID: 24265315
    6. Docking and physicochemical studies indicated that BTK was involved in close contact with Tyr86 and Tyr106 of MAL, whereas PKCdelta may phosphorylate Tyr106 only. PMID: 24840642
    7. Results show that MAL protein binds to and regulates MYD88. PMID: 26876098
    8. Here the s show that MAL TIR domains spontaneously and reversibly form filaments in vitro. They also form cofilaments with TLR4 TIR domains and induce formation of MyD88 assemblies. PMID: 28759049
    9. The major allele A of rs2511521 located in DRD2 and the minor allele T of rs625413 located in TIRAP are significantly associated with increased risk of food addiction in overweight/obese subjects with low/zero food addiction symptoms. PMID: 28115213
    10. Combined targeting of UBAP1 and toll-like receptor adaptors TIRAP and MyD88 by Pseudomonas aeruginosa PumA impedes both cytokine and toll-like receptor signalling, highlighting a novel strategy for innate immune evasion. PMID: 28483816
    11. Epistatic interaction between MyD88 and TIRAP against Helicobacter pylori. PMID: 27296467
    12. Presence of at least one copy of the TIRAP (2054C > T) variant may be associated with severity of bronchopulmonary dysplasia among preterm neonates. PMID: 27457283
    13. Chances of malaria caused by Plasmodium falciparum were low in CC genotype carriers in comparison to other genotypes PMID: 26614847
    14. TIRAP polymorphisms are associated with progression and survival of patients with symptomatic myeloma. PMID: 26564000
    15. This present meta-analysis suggests TIRAP C539T polymorphism is significantly correlated with reduced risk of TB infection, with stronger effect in European. PMID: 25003251
    16. Evaluated the possible association between TIRAP rs1893352 and rs8177374 (S180L) gene polymorphisms and pulmonary tuberculosis in a sample of Iranian population. PMID: 25066393
    17. CCL2rs2530797A/A and TIRAPrs8177376A/A were associated to an increase susceptibility to Chagas disease cardiomyopathy. PMID: 24330528
    18. TIRAP rs81777374 is associated with resistance to pulmonary tuberculosis. PMID: 24067789
    19. Data indicate that TcpB (NP_540591) may mimic the function of TIRAP through their similar TIR domain structures. PMID: 24275656
    20. PSP has an immunoregulatory effect through regulation of the TLR4-TIRAP/MAL-MyD88 signaling pathway. PMID: 23802631
    21. the results of our study suggest that there is a defect of TIRAP and MyD88 proteins in B-CLL lymphocytes. PMID: 23419703
    22. TIRAP S180L polymorphism is not increased in Behcet's disease patients in Italian or Turkish populations. PMID: 24064014
    23. In our study, a TLR pathway adapter variant (TIRAP (rs8177374)) protected against preterm birht<32 weeks, supporting our hypothesis that genetic variation in the innate immune signaling pathway contributes to altered risk of PTB. PMID: 23047423
    24. MyD88 exerts antiapoptotic functions in colon cancer cells via the Ras/Erk, but not the NF-kappaB, pathway. MyD88 inhibition leads to defective ERCC1-dependent DNA repair and to accumulation of DNA damage, resulting in cancer cell death via p53. PMID: 23766530
    25. Identification of binding sites for myeloid differentiation primary response gene 88 (MyD88) and Toll-like receptor 4 in MyD88 adapter-like (Mal). PMID: 23460645
    26. PIP5Kalpha promotes TLR4-associated microglial inflammation by mediating PIP(2)-dependent recruitment of TIRAP to the plasma membrane PMID: 23297396
    27. Compared to white women, African American women with clinically suspected pelvic inflammatory disease were more likely to carry variants in the TLR1, TLR2, and TIRAP genes. PMID: 23255565
    28. induction of both IL-6 and IL-8 is associated with elevated TIRAP and reduced TRAM mRNA expression PMID: 22970235
    29. Data suggest a protection effect of the Mal/TIRAP S180L SNP against serious infections in HIV-1 infected individuals with low CD4 cell counts. PMID: 22683004
    30. Data indicate that MyD88 works together with the IL-1/IL-18 receptors, can interact with two distinct sorting adaptors, TRAM and Mal, in a conserved manner. PMID: 22685567
    31. Poxviral protein A46 inhibits TLR4 signaling and interacts with Toll-IL-1 receptor (TIR) domain-containing proteins of the receptor complex. PMID: 22593572
    32. TIRAP S180L polymorphism is unlikely to substantially contribute to tuberculosis susceptibility (Meta-analysis) PMID: 21419702
    33. Blocking of the function of TIRAP and MyD88 largely abrogated intracellular signaling from ligand-activated RAGE PMID: 21829704
    34. TIRAP G286A (D96N) polymorphism is associated with susceptibility to tuberculosis. PMID: 21218381
    35. TIRAP 180Leu was significantly associated with Behcet's disease in UK, but not Middle Eastern, patients. PMID: 21705416
    36. a molecular and structural basis for the role of MAL in TLR signaling and disease protection. PMID: 21873236
    37. RA synovium showed abundant expression of TLR. RA synovitis tissue seems to be responsive to TLR ligands. PMID: 21324962
    38. Comparison of the tirap S180L genotypes between patients with mild malaria and those healthy individuals showed that the frequency of heterozygosity was significantly higher in infected than non-infected individuals. PMID: 21457584
    39. Data demonstrate a key role for Mal in mediating TLR2 and TLR4 activation of CREB and induction of CREB-responsive genes including Il-10. PMID: 21398611
    40. Genetic variations in MyD88 adaptor-like are associated with atopic dermatitis PMID: 21399862
    41. TIRAP gene might be associated with susceptibility to sepsis-associated acute lung injury in the Han Chinese population. PMID: 21118491
    42. Patients simultaneously carrying polymorphisms in TIRAP/Mal and TLR4 and patients homozygous for the TIRAP/Mal SNP had a significantly higher risk of severe infections after surgery PMID: 20525286
    43. The recessive homozygous genotype for a single-nucleotide polymorphisms in the TIRAP (also known as MAL) gene (rs1893352) was strongly associated with nonmeningitis cases of haemophilus influenzae serotype b vaccine failure. PMID: 20804371
    44. An Asp96Asn single nucleotide polymorphism prevents Mal from recruiting its signaling partner MyD88 to the plasma membrane and fails to induce NF-kappa B signaling in human tumor line Huh-7 hepatocytes. PMID: 20164415
    45. Data show that AIP1 is a novel GTPase-activating protein for Arf6, a small GTPase regulating cellular PIP(2) production and formation of the TLR4-TIRAP-MyD88 complex. PMID: 19948740
    46. A TIR domain variant of MyD88 adapter-like (Mal)/TIRAP results in loss of MyD88 binding and reduced TLR2/TLR4 signaling. PMID: 19509286
    47. Polymorphisms in TIRAP do not appear to be involved in childhood tuberculosis susceptibility in South Africa, but may play a role in determining occurrence of tubercular meningitis PMID: 19693265
    48. MyD88 and Mal/TIRAP are essential for LPS-induced I kappa B alpha phosphorylation, NF-kappa B activation, and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2. PMID: 14630816
    49. involvement of a previously uncharacterized, evolutionarily conserved TIR domain protein in innate immunity that is functionally distinct from other known TIR domain signaling adapters PMID: 15123841
    50. activation of Rac1 leads to HIV-LTR trans-activation, mediated through TIRAP. Rac1 and TIRAP are important in TLR4 activation of HIV replication PMID: 15187145

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Membrane. Note=Colocalizes with DAB2IP at the plasma membrane.
  • 组织特异性：
    Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
  • 数据库链接：

    HGNC: 17192

    OMIM: 606252

    KEGG: hsa:114609

    STRING: 9606.ENSP00000376445

    UniGene: Hs.537126