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  4. Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1)

Recombinant Human Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1)

  • 中文名称：
    人MAPKAP1重组蛋白
  • 货号：
    CSB-EP857767HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP857767HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MAPKAP1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP857767HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MAPKAP1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    MAPKAP 1; MAPKAP1; MEKK2 interacting protein 1; MGC2745; MIP 1; MIP1; Mitogen activated protein kinase associated protein 1; Mitogen-activated protein kinase 2-associated protein 1; mSIN1; OTTHUMP00000064207; Ras inhibitor MGC2745; SAPK interacting protein 1; SAPK-interacting protein 1; SIN 1; SIN1_HUMAN; SIN1b; SIN1g; Stress activated map kinase interacting protein 1; Stress activated protein kinase interacting 1; Stress-activated map kinase-interacting protein 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    75.0kDa
  • 表达区域：
    2-522aa
  • 氨基酸序列
    AFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
  • 基因功能参考文献：
    1. SIN1 interacted and co-located with PKC zeta by pleckstrin homology (PH) domain. Downregulation of SIN1 severely impaired PKC zeta translocation and phosphorylation induced by insulin. PMID: 28751630
    2. SIN1 plays an important role in breast cancer. PMID: 27780891
    3. In colorectal cancer tissues, the Sin1 protein but not mRNA was significantly upregulated while Pdcd4 protein was downregulated, suggesting that loss of Pdcd4 might correlate with Sin1 protein level but not mRNA level in colorectal cancer. PMID: 28692058
    4. SIN1 plays an important role in non-small cell lung cancer; SIN1 is a potential biomarker and a promising target in the treatment of NSCLC PMID: 27993679
    5. This study provides evidence that Sin1, a known element of the mammalian target of rapamycin complex 2 (mTORC2), is required for Interferon-gamma-induced phosphorylation and activation of AKT and that such activation mediates downstream regulation of mTORC1 and its effectors. PMID: 28174303
    6. Intracellular localization of mTORC2 component, mSin1, contributes to regulation of Akt phosphorylation. PMID: 28143890
    7. Akt phosphorylates SIN1 at T86, enhancing mTORC2 kinase activity, which leads to phosphorylation of Akt S473 by mTORC2, thereby catalyzing full activation of Akt. PMID: 26235620
    8. MAPKAP1 may represent a novel anti-infection and anti-fibrogenesis genomic locus in chronic schistosomiasis japonica. PMID: 25153992
    9. mitogen-activated protein kinase associated protein 1 rs10118570 may be an important protective factor for developing better management strategies in lung squamous cell carcinoma. PMID: 24926550
    10. DNA-PKcs-mTORC2(SIN1) association is required for UVB-induced Akt Ser-473 phosphorylation and cell survival. PMID: 24365180
    11. Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient lymphangioleiomyomatosis cells. PMID: 24395886
    12. results reveal a Sin1-phosphorylation-dependent mTORC2 regulation, providing a potential molecular mechanism by which mutations in the mTORC1-S6K-Sin1 signalling axis might cause aberrant hyper-activation of the mTORC2-Akt pathway PMID: 24161930
    13. NBS1 interacts with the mTOR/Rictor/SIN1 complex through the a.a. 221-402 domain and contributes to the activation of Akt activity. PMID: 23762398
    14. SIN1 plays an important role in hepatocellular carcinoma invasion and metastasis by facilitating epithelial-mesenchymal transition. PMID: 23564492
    15. structures show that the C-termini of Avo1 and Sin1 both have the pleckstrin homology (PH) domain fold PMID: 22505404
    16. mSIN1 protein mediates SGK1 protein interaction with mTORC2 protein complex and is required for selective activation of the epithelial sodium channel PMID: 21757730
    17. There is an increased mitochondrial dependence upon mTORC2 dependent cell growth due to PTEN loss PMID: 21170086
    18. Results demonstrate that Sin1 transcripts can use alternative polyadenylation signals and describe a number of Sin1 splice variants that potentially encode functionally different isoforms. PMID: 15363842
    19. The human Sin1 proteins form a complex with Jun N-terminal kinase. PMID: 15722200
    20. A novel MEKK2-interacting protein, Mip1, that regulates MEKK2 dimerization and activation by forming a complex with inactive and nonphosphorylated MEKK2, was identified. PMID: 15988011
    21. Sin1 is necessary for the assembly of TORC2 and for its capacity to phosphorylate Akt/PKB. PMID: 16919458
    22. Results reveal that the SIN1-rictor-mTOR function in Akt-Ser473 phosphorylation is required for TORC2 function in cell survival but is dispensable for TORC1 function. PMID: 16962653
    23. Sin1 together with Rictor are key components of mTORC2 and play an essential role in Akt phosphorylation and signaling PMID: 17043309
    24. Sin1 may contribute to ATF-2 signaling specificity by acting as a nuclear scaffold. PMID: 17054722
    25. These data suggest that Sin1 is a mammalian Ras-inhibitor. PMID: 17303383
    26. MIP-1 delta expression is increased in RBM (RCC (Renal Cell Carcinoma) bone metastasis) relative to RCC and bone marrow, and may promote RBM-induced osteolysis by stimulating recruitment/differentiation of osteoclast precursors into mature osteoclasts PMID: 18316587
    27. SIN1 and PCBP2 are generally coregulated with large numbers of genes implicated in both cell survival and death and in cellular stress responses, including RNA translation and processing PMID: 18687895

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.
  • 蛋白家族：
    SIN1 family
  • 组织特异性：
    Ubiquitously expressed, with highest levels in heart and skeletal muscle.
  • 数据库链接：

    HGNC: 18752

    OMIM: 610558

    KEGG: hsa:79109

    STRING: 9606.ENSP00000265960

    UniGene: Hs.495138