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  4. Recombinant Human TBC1 domain family member 10A (TBC1D10A)

Recombinant Human TBC1 domain family member 10A (TBC1D10A)

  • 中文名称：
    人TBC1D10A重组蛋白
  • 货号：
    CSB-YP880127HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TBC1D10A重组蛋白
  • 货号：
    CSB-EP880127HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TBC1D10A重组蛋白
  • 货号：
    CSB-EP880127HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TBC1D10A重组蛋白
  • 货号：
    CSB-BP880127HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TBC1D10A重组蛋白
  • 货号：
    CSB-MP880127HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TBC1D10A
  • Uniprot No.：
  • 别名：
    dJ130H16.1; dJ130H16.2; EBP50 PDZ interactor of 64 kD; EBP50-PDX interactor of 64 kDa; EPI 64; EPI64; EPI64 protein; Rab27A-GAP-alpha; TB10A_HUMAN; TBC1 domain family member 10A; TBC1D10; TBC1D10A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-508
  • 氨基酸序列
    MAKSNGENGP RAPAAGESLS GTRESLAQGP DAATTDELSS LGSDSEANGF AERRIDKFGF IVGSQGAEGA LEEVPLEVLR QRESKWLDML NNWDKWMAKK HKKIRLRCQK GIPPSLRGRA WQYLSGGKVK LQQNPGKFDE LDMSPGDPKW LDVIERDLHR QFPFHEMFVS RGGHGQQDLF RVLKAYTLYR PEEGYCQAQA PIAAVLLMHM PAEQAFWCLV QICEKYLPGY YSEKLEAIQL DGEILFSLLQ KVSPVAHKHL SRQKIDPLLY MTEWFMCAFS RTLPWSSVLR VWDMFFCEGV KIIFRVGLVL LKHALGSPEK VKACQGQYET IERLRSLSPK IMQEAFLVQE VVELPVTERQ IEREHLIQLR RWQETRGELQ CRSPPRLHGA KAILDAEPGP RPALQPSPSI RLPLDAPLPG SKAKPKPPKQ AQKEQRKQMK GRGQLEKPPA PNQAMVVAAA GDACPPQHVP PKDSAPKDSA PQDLAPQVSA HHRSQESLTS QESEDTYL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A.
  • 基因功能参考文献：
    1. Data suggest that Rab35, interacting with TBC1D10A, functions in vascular endothelial cells as a negative regulator of histamine-evoked, Ca2+-dependent Weibel-Palade body exocytosis, most likely acting through the downstream effectors ACAP2 and Arf6. (Rab35 = rab GTP-binding protein 53; TBC1D10A = TBC1 domain family member 10A; ACAP2 = centaurin beta2; Arf6 = ADP-ribosylation factor 6) PMID: 28566286
    2. EPI64, a candidate GAP that is specific for Rab27. PMID: 24673604
    3. Data suggest that EPI64A and B, which are ubiquitously expressed members of the EPI64 subfamily, inactivate Ras and certain Rabs at the periphery of cells. PMID: 23248241
    4. EPI64 regulates membrane trafficking both by stabilizing Arf6-GTP and by inhibiting the recycling of membrane through the tubular endosome by decreasing Rab8a-GTP levels. PMID: 22219378
    5. analysis of recycling of the Ca2+-activated K+ channel, KCa2.3, is dependent upon RME-1, Rab35/EPI64C, and an N-terminal domain PMID: 20360009
    6. EPI64 is a GTPase-activating protein specific for Rab27A PMID: 16923811
    7. These data reveal that microvilli have distinct cytoskeletal subdomains and that EPI64 regulates microvillar structure. PMID: 17145964
    8. EPI64C and Rab35 regulate a recycling pathway in T cells and contribute to immunological synapse formation, most likely by participating in TCR transport to the immunological synapse PMID: 18450757

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  • 亚细胞定位：
    Cell projection, microvillus. Note=Localizes to the microvilli-rich region of the syncytiotrophoblast. In melanocytes, located at the periphery of cells.
  • 数据库链接：

    HGNC: 23609

    OMIM: 610020

    KEGG: hsa:83874

    STRING: 9606.ENSP00000384996

    UniGene: Hs.655273