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  4. Recombinant Human T cell receptor alpha chain constant (TRAC)

Recombinant Human T cell receptor alpha chain constant (TRAC)

In Stock
  • 中文名称：
    人TRAC重组蛋白
  • 货号：
    CSB-EP024144HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    TRAC
  • Uniprot No.：
  • 别名：
    TRAC; TCRA; T cell receptor alpha constant
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    19.9kDa
  • 表达区域：
    1-142aa
  • 氨基酸序列
    PNIQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The Recombinant Human TRAC protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of TRAC gene. This recombinant TRAC protein was expressed in the host. The expression region is 1-142aa of the Human TRAC. In the production, the expression vector contains N-terminal 6xHis tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.

    The TRAC gene encodes for the constant region of the TCR subunit and is essential for membrane expression of the TCRαβ heterodimer. In recent years, researcher revealed the important roles of TRAC in different diseases. For example, it has been found that mutation in the TCRα subunit constant gene leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cells. Besides, narcolepsy is strongly associated with the T-cell receptor alpha locus. Furthermore, gene rearrangements and lymphoma are partly responsible for a pathogenic TRAC Variant. Previous studies have shown that mutation in TCRα subunit constant (TRAC) leads to loss of αβ TCR on T-cell surface. Beyond this, some findings indicate that CRISPR-Cas9-mediated multiplex gene editing of TRAC, B2M, and PD-1 in CAR-T cells. The reports further adds that although these double (TRAC/B2M)- and triple (TRAC/B2M/PD-1)-negative CAR-T cells need to be further tested for their safety and efficacy in clinical studies, our results suggest that they are promising reagents for cancer therapy.

     

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.
  • 相关疾病：
    Immunodeficiency 7 (IMD7)
  • 亚细胞定位：
    Cell membrane.
  • 数据库链接：

    HGNC: 12029

    OMIM: 186880

    UniGene: Hs.74647