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  4. Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2)

Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2)

  • 中文名称：
    人U2AF2重组蛋白
  • 货号：
    CSB-YP025408HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人U2AF2重组蛋白
  • 货号：
    CSB-EP025408HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人U2AF2重组蛋白
  • 货号：
    CSB-BP025408HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人U2AF2重组蛋白
  • 货号：
    CSB-MP025408HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    U2AF2
  • Uniprot No.：
  • 别名：
    hU2AF(65); hU2AF65; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit; U2af2; U2AF2_HUMAN; U2AF65
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-475
  • 氨基酸序列
    SDFDEFERQ LNENKQERDK ENRHRKRSHS RSRSRDRKRR SRSRDRRNRD QRSASRDRRR RSKPLTRGAK EEHGGLIRSP RHEKKKKVRK YWDVPPPGFE HITPMQYKAM QAAGQIPATA LLPTMTPDGL AVTPTPVPVV GSQMTRQARR LYVGNIPFGI TEEAMMDFFN AQMRLGGLTQ APGNPVLAVQ INQDKNFAFL EFRSVDETTQ AMAFDGIIFQ GQSLKIRRPH DYQPLPGMSE NPSVYVPGVV STVVPDSAHK LFIGGLPNYL NDDQVKELLT SFGPLKAFNL VKDSATGLSK GYAFCEYVDI NVTDQAIAGL NGMQLGDKKL LVQRASVGAK NATLVSPPST INQTPVTLQV PGLMSSQVQM GGHPTEVLCL MNMVLPEELL DDEEYEEIVE DVRDECSKYG LVKSIEIPRP VDGVEVPGCG KIFVEFTSVF DCQKAMQGLT GRKFANRVVV TKYCDPDSYH RRDFW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.
  • 基因功能参考文献：
    1. Data show that trans-acting RNA-binding proteins (RBPs) extensively regulate U2 (RNU2) small nuclear RNA auxiliary factor 2 protein (U2AF2) binding in vivo, including enhanced recruitment to 3' splice sites and clearance of introns PMID: 29643205
    2. Data suggest that one way heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) regulates exon definition is to modulate the interaction of U2 small nuclear RNA auxiliary factor 2 (U2AF2) with decoy or bona fide 3' splice site (3'ss). PMID: 29650551
    3. In summary, we provide insight into the underlying molecular mechanism of U2AF binding to 3' splice sites. PMID: 27799531
    4. the most discriminant differences between lincRNAs and mRNAs involve splicing. LincRNAs are less efficiently spliced, which cannot be explained by differences in U1 binding or the density of exonic splicing enhancers but may be partially attributed to lower U2AF65 binding and weaker splicing-related motifs. PMID: 27927715
    5. RBM39 is extensively involved in alternative splicing of RNA and helps regulate transcript levels. RBM39 may modulate alternative splicing similarly to U2AF65 by either directly binding to RNA or recruiting other splicing factors, such as U2AF65. PMID: 27354116
    6. These findings uncovered a hitherto unappreciated function of CD82 in severing the linkage between U2AF2-mediated CD44 alternative splicing and cancer aggressiveness, with potential prognostic and therapeutic implications in melanoma PMID: 27041584
    7. JMJD6 regulated a large set of alternative splicing events, and importantly, its regulated splicing events were significantly overlapped those controlled by U2AF65. PMID: 27899633
    8. This study therefore establishes a structural basis for specific UHM-ULM interactions by splicing factors such as U2AF35, U2AF65, RBM39 and SF3b155, and a platform for continued studies of intermolecular interactions governing disease-related alternative splicing in eukaryotic cells. PMID: 27050129
    9. The U2AF(65) linker residues between the dual RNA recognition motifs (RRMs) recognize the central nucleotide, whereas the N- and C-terminal RRM extensions recognize the 3' terminus and third nucleotide. PMID: 26952537
    10. Nuclear retention of full-length HTT RNA is mediated by splicing factors MBNL1 and U2AF65 PMID: 26218986
    11. Serum RNU2-1f may serve as a biomarker for lung cancer detection, prognosis prediction and treatment monitoring. PMID: 26687686
    12. U2AF(65) possesses a splicing Inhibitory function that leads to alternative exon skipping. PMID: 26216990
    13. The spatial distribution of U2AF65 conformations is found to be highly anisotropic, comprising significantly populated interdomain contacts that appear to be electrostatic in origin. PMID: 24734879
    14. reduced U2AF(65) binding is a molecular consequence of disease-relevant mutations, and a structure-guided U2AF(65) variant is capable of manipulating gene expression in eukaryotic cells PMID: 25422459
    15. We demonstrated that PRPF40B interacts directly with SF1 and associates with U2AF(65 PMID: 25605964
    16. U2AF has the capacity to directly define ~88% of functional 3' splice sites in the human genome; numerous U2AF binding events also occur in intronic locations. PMID: 25326705
    17. U2AF65 represents a new route for modulating TRF1 function at telomeres. PMID: 24389012
    18. The conserved SPSP motif phosphorylation and the SF1/U2AF interface are essential in vivo. PMID: 23273425
    19. The results highlight both local and global conformational selection as a means for universal 3' splice site recognition by U2AF65. PMID: 23376934
    20. The REST N exon is a very versatile sequence with a complex array of splicing signals, and its activation in H69 cells depends on the relative amounts of hnRNP H and U2AF65. PMID: 22792276
    21. By preventing U2AF65 binding to Alu elements, hnRNP C plays a critical role as a genome-wide sentinel protecting the transcriptome. PMID: 23374342
    22. These complementary structural methods demonstrate that diverse splice sites have the opportunity to select compact or extended inter-RRM proximities from the U2AF65 conformational pool PMID: 22702716
    23. hnRNP A1 forms a ternary complex with the U2AF heterodimer on AG-containing/uridine-rich RNAs, while it displaces U2AF from non-AG-containing/uridine-rich RNAs, an activity that requires the glycine-rich domain of hnRNP A1 PMID: 22325350
    24. dephosphorylation of pS776-ATXN1 by PP2A regulates the interaction of ATXN1 with the splicing factors RBM17 and U2AF65 PMID: 21835928
    25. the molecular mechanisms of the recognition of the 3'-splice-site-associated polypyrimidine tract RNA by the large subunit of the human U2 snRNP auxiliary factor (U2AF65) as a key early step in pre-mRNA splicing PMID: 21753750
    26. U2AF65 binds directly to the phosphorylated C-terminal domain, and that this interaction results in increased recruitment of U2AF65 and PRP19C to the pre-mRNA PMID: 21536736
    27. the conformational changes that are induced by assembly of the SF1/U2AF(65)/RNA complex serve to position the pre-mRNA splice site optimally for subsequent stages of splicing. PMID: 21146534
    28. U2AF 65 promotes 3'-end processing, which contributes to 3'-terminal exon definition in RNA splicing PMID: 12189174
    29. U2AF35 RRM is unstructured in solution but its tertiary structure is induced upon binding to U2AF65. PMID: 12297299
    30. U2AF65 modulates the function of the PLE (poly(A)-limiting element) PMID: 14576315
    31. two members of the U2AF65 family of proteins, hCC1.3, which we call CAPERalpha, and a related protein, CAPERbeta, regulate both steroid hormone receptor-mediated transcription and alternative splicing PMID: 15694343
    32. identified and spatially localized sites of direct interaction between U2AF35 and U2AF65 in vivo in live cell nuclei. PMID: 16043505
    33. Multiple U2AF65 binding sites within SF3b155 regulate conformational rearrangements during spliceosome assembly. PMID: 16376933
    34. Cocrystals of a U2AF65 variant with a deoxyuridine dodecamer diffract X-rays to 2.9 angstroms resolution and contain one complex per asymmetric unit. PMID: 16682775
    35. DEK enforces 3' splice site discrimination by U2AF; DEK phosphorylated at serines 19 and 32 associates with U2AF35, facilitates the U2AF35-AG interaction and prevents binding of U2AF65 to pyrimidine tracts not followed by AG PMID: 16809543
    36. Results describe the roles of the two subunits of U2AF, U2AF65 and 35, in the selection between alternative 3' splice sites associated with polypyrimidine tracts of different strengths. PMID: 16940179
    37. U2AF65 associates with specific subsets of spliced mRNAs, strongly suggesting that it is involved in novel cellular functions in addition to splicing. PMID: 17137510
    38. This rare, high-resolution view of an important member of the RNA recognition motifs class of RNA-binding domains highlights the role of alternative side-chain conformations in RNA recognition. PMID: 17188295
    39. SF1 and U2AF form extraspliceosomal complexes before and after taking part in the assembly of catalytic spliceosomes. PMID: 18285458
    40. there is a functional link among apoptosis induction, U2AF65 cleavage, and the regulation of Fas alternative splicing PMID: 18508922
    41. Solution conformation and thermodynamic characteristics of RNA binding by the splicing factor U2AF65. PMID: 18842594
    42. Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155. PMID: 18974054
    43. Collectively, the results suggest that both U2AF binding and other steps of U2 snRNP recruitment can be control points in SMN splicing regulation. PMID: 19244360
    44. study reveals the splicing factor U2AF65 undergoes posttranslational lysyl-5-hydroxylation catalyzed by Jmjd6 PMID: 19574390

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Splicing factor SR family
  • 数据库链接：

    HGNC: 23156

    OMIM: 191318

    KEGG: hsa:11338

    STRING: 9606.ENSP00000307863

    UniGene: Hs.528007