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  4. Recombinant Human Serine/threonine-protein phosphatase 2A activator (PPP2R4)

Recombinant Human Serine/threonine-protein phosphatase 2A activator (PPP2R4)

  • 中文名称：
    人PTPA重组蛋白
  • 货号：
    CSB-EP620876HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PTPA
  • Uniprot No.：
  • 别名：
    subunit B''; KIAA0044; MGC2184; Phosphotyrosyl phosphatase activator; PP2A; PP2A phosphatase activator; PP2A subunit B'; PP2A subunit B' isoform PR53; PP2A subunit B' PR53 isoform; PPP2R4; PR53; PR53 isoform; Protein phosphatase 2, regulatory subunit B-prime; Protein phosphatase 2A activator regulatory subunit 4; Protein phosphatase 2A regulatory subunit B' (PR 53); PTPA; PTPA_HUMAN; Serine/threonine protein phosphatase 2A regulatory subunit B'; Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B''
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    60.5kDa
  • 表达区域：
    2-358aa
  • 氨基酸序列
    AEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEMWNEVHEEKEQAAKQSVSCDECIPLPRAGHCAPSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
  • 基因功能参考文献：
    1. PP2A dephosphorylates MYPT1(pThr696) and thereby stimulates MP activity inducing dephosphorylation of eNOS(pThr497) and the 20 kDa myosin II light chains. PMID: 28300193
    2. Study reveals the mechanism controlling abscission through integration of Aurora B kinase and B56-bound PP2A phosphatase activities on the kinesin motor protein MKlp2. MKlp2 is an essential protein for promoting abscission, which may regulate tethering and stabilizing of the PM to the microtubule cytoskeleton at the intercellular bridge through its previously uncharacterized lipid association motif. PMID: 27939310
    3. These results suggest that loss of specific PP2A regulatory subunits is functionally important in breast tumourigenesis, and support strategies to enhance PP2A activity as a therapeutic approach in breast cancer. PMID: 28744751
    4. High expression of PP2A is associated with cisplatin resistant gastric cancer. PMID: 28534965
    5. Study identifies Eya3 as a regulator of PP2A, a major cellular Ser/Thr phosphatase, and uncovers a mechanism of controlling the stability of a critical oncogene, c-Myc. PMID: 29535359
    6. s demonstrated CFTR and PP2AA interact in the cytosol, resulting in PP2A complex inactivation and increased degradation of PP2A substrates via the lysosomal/proteasome pathway. PMID: 28445932
    7. Together, the results suggest a complex antagonistic interplay between the control of ARPP-16 by MAST3 and PKA that creates a mechanism whereby cAMP mediates PP2A disinhibition. PMID: 28613156
    8. An imbalanced regulation in protein kinases and protein phosphatases is the direct cause of tau hyperphosphorylation in Alzheimer's disease; GSK-3beta and PP2A are the most implicated. (Review) PMID: 28585125
    9. Isoliensinine suppresses NF-kappaB in hepatocellular carcinoma cells through impairing PP2A/I2PP2A interaction and stimulating PP2A-dependent p65 dephosphorylation at Ser536 PMID: 27244888
    10. BIR domain of XIAP activated the protein phosphatase 2 (PP2A) activity by decreasing the phosphorylation of PP2A at Tyr307 in its catalytic subunit, PP2A-C. Such activated PP2A prevented the deviant phosphorylation and activation of MAPK kinases/MAPKs, their downstream effector c-Jun; and in turn inhibiting transcription of c-Jun-regulated miR-200a PMID: 28057023
    11. T-type channel signaling is redirected towards the activation of the kinase Akt1, leading to increased expression of the anti-apoptotic protein survivin, and a decrease in the pro-apoptotic mediator FoxO3A. Finally, in iPAH cells, Akt1 is no longer able to regulate caspase 9 activation, whereas T-type channel overexpression reverses PP2A defect in iPAH cells but reinforces the deleterious effects of Akt1 activation PMID: 28655554
    12. The present results indicate that rs959627 predicts PPP2R2 B mRNA prefrontal expression in two independent post-mortem datasets as well as lateral prefrontal activity during working memory in healthy subjects and suggest that genetic modulation of signal transduction mediated by PP2A affects complex biological phenotypes of relevance for cognitive behavior. PMID: 27591184
    13. the present study suggested that PP2A has an important role in regulating mast cell beta2-adrenoceptors PMID: 28090813
    14. The results of this study demonstrated that HBx of hepatitis B virus impairs interferon signaling via increased expression of SOCS3 and PP2A. PMID: 27459003
    15. The current study describes a novel pathogenic mechanism of action for human polyomavirus 6 small tumor (sT) antigen which involves binding to protein phosphatase 2A (PP2A) via its WFG motif and zinc binding sites for activation of PP2A's downstream oncogenic pathways (MEK/ERK/c-Jun). PMID: 27632801
    16. The aim of this review is to shed light on the role of four different phosphatases (PTEN, PP2A, CDC25 and DUSP1) in five different solid tumors (breast cancer, lung cancer, pancreatic cancer, prostate cancer and ovarian cancer), in order to better understand the most frequent and aggressive primary cancer of the central nervous system, glioblastoma. PMID: 28801478
    17. Oncoprotein CIP2A is stabilized via interaction with tumor suppressor PP2A/B56 PMID: 28174209
    18. Such effect by miR-429 was again abolished with AMPKalpha1 silence or mutation. Together, we propose that PP2A-c silence by miR-429 activates AMPK and protects osteoblastic cells from Dex. PMID: 28438603
    19. PTPalpha is identified as a novel substrate of N-Acetylglucosaminyltransferase V (GnT-V) and could be a factor regulating promotion of migration in breast cancer cells PMID: 27965091
    20. This article reviews the involvement of PP2A in the regulation of three cell signaling pathways: wnt, mTOR and MAP kinase, as well as the G1-->S transition, DNA synthesis and mitotic initiation. These processes are all crucial for proper cell survival and proliferation and are often deregulated in cancer and other diseases. [review] PMID: 26906453
    21. PP2A-B55 substrates were identified and assigned their temporal order in mitotic exit. PMID: 27551054
    22. Our results provide a novel mechanistic insight into the activation of Myc by 27-HC via transcriptional repression of PP2A, SCP1 and FBW7 to increase Myc protein stability in breast cancer cells. PMID: 27751849
    23. Data show that the truncation promotes glycogen synthase kinase-3beta (GSK-3beta) nuclear translocation and enhances its interaction with protein phosphatase 2A (PP2A), leading to dephosphorylation. PMID: 28267204
    24. that KAP1 phosphorylation is decreased following recruitment of PP2A by URI. PMID: 27780869
    25. These results provide direct evidence for the rapid response of PP2A catalytic activity to extracellular insulin stimulation, as well as insight into the complex regulation of phosphorylation levels by opposing kinase and phosphatase activities within the cell. PMID: 27805358
    26. LPS tolerance interferes with TLR4 signaling by inhibiting Lyn and c-Src phosphorylation and their recruitment to TLR4, while increasing the phosphatase activity and expression of PP2A, PTPN22, PTP1B and MKP1. PMID: 26457672
    27. we propose that RhoB could be responsible for early metastatic prevention by inhibiting the EMT-derived invasiveness of lung cells through the control of PP2A activity. PMID: 26148238
    28. Cohesin complex is shown to be a target of the prophase pathway at centrosomes and protected by Sgo1-dependent PP2A recruitment. PMID: 26365192
    29. Zinc-alpha2-glycoprotein modulates AKT-dependent insulin signaling in adipocytes by activation of the PP2A phosphatase. PMID: 26068931
    30. these data reveal the molecular mechanisms of PP2A regulation and highlight the potential of boosting the power of endogenous phosphatases as novel anti-inflammatory strategies to combat asthmatic inflammation. PMID: 25985190
    31. Transgenic overexpression of myocardial PP2A causes adverse remodeling which coincides with a disruption of the classical Akt/GSK3/beta-catenin pathway under baseline conditions that is restored to normal values in chronic myocardial infarction. PMID: 25662052
    32. an increase in the phosphorylation level of PTPalphaTyr789 by its upstream activator, FAKDel33, leading to Src activation in certain breast cancer cells, which has significant implications for metastatic potential. PMID: 25625869
    33. results suggested that PPFIA1 functioned with PP2A to promote the dephosphorylation of Kif7, triggering Kif7 localization to the tips of primary cilia and promoting Gli transcriptional activity. PMID: 25492966
    34. The roles of protein phosphatases including MAPK phosphatase-1 (MKP-1) and protein phosphatase type 2A (PP2A) in modulating p38MAPK activation and downstream TNF-alpha expressions in primary human monocyte-derived macrophages, were examined. PMID: 23549267
    35. The invariant C-terminal residues of the catalytic subunit can act as affinity enhancer for different PP2A interaction partners, including PTPA, and a different 'code' of posttranslational modifications can favour interactions to one subunit over others. PMID: 25003389
    36. REDD1 may act as a targeting protein for the catalytic subunit of PP2A PMID: 25056877
    37. We have also defined in the actin-binding protein cofilin-1 a link between PP2A, actin cytoskeleton, and necrotic death PMID: 25096578
    38. PP2A is activated upon CK2 activation and acts as the dephosphorylating agent for pStat3S727. PMID: 24726840
    39. we investigated whether down-regulation of PTPA affects cell viability and the underlying mechanismswe investigated whether down-regulation of PTPA affects cell viability and the underlying mechanisms PMID: 24821282
    40. Hyperphosphorylation of PP2A is associated with colorectal cancer. PMID: 24997451
    41. PTPA stabilizes a conformation of apo- PP2Ac that facilitates metal chelation and ATP binding and hydrolysis, whereby the precise geometry of ATP binding at the active site is important for selectively enhanced binding of proper catalytic metal ions. PMID: 24100351
    42. These data point to PP2A-B56gamma-driven Ras signaling modulation in G2 as essential for suppressing aberrant cyclin E expression during mitosis and thereby achieving normal G0 control. PMID: 24857551
    43. The presence of full-length SET in the neuronal cytoplasm is sufficient to impair PP2A methylation and activity, leading to tau hyperphosphorylation. PMID: 24981783
    44. the A/Balpha/C holoenzyme form of PP2A is essential in EC barrier integrity both in micro- and macrovascular EC PMID: 23721711
    45. s show that PP2Ac associates with Jak1/Tyk2/STAT1 and reduces Jak1/Tyk2/STAT1 phosphorylation resulting in an impairment of the IFNalpha-induced hepatitis C virus antiviral response. PMID: 23910645
    46. Plant homeodomain finger protein 20 (PHF20) maintains NF-kappaB in an active state in the nucleus by inhibiting the interaction between PP2A and p65. PMID: 23797602
    47. results suggest that Notch1 can regulate PP2A dephosphorylation of critical cellular regulators including AKT, AMPKalpha, and p70S6K PMID: 23788636
    48. results identify downregualtion of PP2A activity as an important protective mechanism of melanoma cells against ER stress-induced apoptosis PMID: 22739989
    49. Our data indicate that PTPA may activate PP2A through activating PTP1B and thus reducing the level of P-PP2AC, therefore upregulation of PTPA may represent a potential strategy in rescuing PP2A and arresting tau pathology in AD. PMID: 23428800
    50. expanded CAG repeats bind to a translation regulatory protein complex containing MID1, protein phosphatase 2A and 40S ribosomal S6 kinase. PMID: 23443539

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    PTPA-type PPIase family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 9308

    OMIM: 600756

    KEGG: hsa:5524

    UniGene: Hs.400740