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  4. Recombinant Human Serine/threonine-protein kinase VRK1 (VRK1)

Recombinant Human Serine/threonine-protein kinase VRK1 (VRK1)

  • 中文名称：
    人VRK1重组蛋白
  • 货号：
    CSB-YP857466HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人VRK1重组蛋白
  • 货号：
    CSB-EP857466HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人VRK1重组蛋白
  • 货号：
    CSB-EP857466HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人VRK1重组蛋白
  • 货号：
    CSB-BP857466HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人VRK1重组蛋白
  • 货号：
    CSB-MP857466HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VRK1
  • Uniprot No.：
  • 别名：
    MGC117401; MGC138280; MGC142070; PCH1; PCH1A; Serine/threonine protein kinase VRK1; Serine/threonine-protein kinase VRK1; Vaccinia related kinase 1; Vaccinia virus B1R related kinase 1; Vaccinia-related kinase 1; VRK1; VRK1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-396
  • 氨基酸序列
    MPRVKAAQAG RQSSAKRHLA EQFAVGEIIT DMAKKEWKVG LPIGQGGFGC IYLADMNSSE SVGSDAPCVV KVEPSDNGPL FTELKFYQRA AKPEQIQKWI RTRKLKYLGV PKYWGSGLHD KNGKSYRFMI MDRFGSDLQK IYEANAKRFS RKTVLQLSLR ILDILEYIHE HEYVHGDIKA SNLLLNYKNP DQVYLVDYGL AYRYCPEGVH KEYKEDPKRC HDGTIEFTSI DAHNGVAPSR RGDLEILGYC MIQWLTGHLP WEDNLKDPKY VRDSKIRYRE NIASLMDKCF PEKNKPGEIA KYMETVKLLD YTEKPLYENL RDILLQGLKA IGSKDDGKLD LSVVENGGLK AKTITKKRKK EIEESKEPGV EDTEWSNTQT EEAIQTRSRT RKRVQK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
  • 基因功能参考文献：
    1. that knockdown of VRK1 would inhibit the proliferation of glioma cells PMID: 29103766
    2. this study not only provides a resource for investigating the unidentified functions of VRK1/VRK3, but also an insight into the regulatory roles of VRK1/VRK3 in biological processes. PMID: 28927264
    3. Block the activation of the VRK1 promoter by Sox2. PMID: 27334688
    4. VRK1 and BANF1 yielded AUCs of 0.790 and 0.735, respectively. PMID: 28298069
    5. VRK1 AND VRK2 expression are predictive of tumor response to neoadjuvant chemoradiation therapy in rectal adenocarcinoma. PMID: 27456229
    6. VRK1 was lowly expressed in adherent MM cells and highly expressed in suspended cells. In addition, VRK1 was positively correlated with the proliferation of MM cells by regulating the expression of cell cycle-related protein, such as cyclinD1, CDK2 and p27(kip1). PMID: 27319807
    7. we conclude that VRK1 may be a candidate prognostic biomarker as well as a potential therapeutical target of hepatocellular carcinoma PMID: 26706601
    8. VRK1 may act as a tumor promoter by controlling the level of cell cycle regulators associated with G1/S transition and could potentially serve as a therapeutic target and/or prognostic biomarker for HCC. PMID: 26375549
    9. VRK1 is a novel regulator of CBs dynamics and stability in cell cycle by protecting coilin from ubiquitination and degradation in the proteasome, and propose a model of CB dynamics. PMID: 26068304
    10. VRK1 regulation of NBS1 contributes to the stability of the repair complex and permits the sequential steps in DNA damage response. PMID: 26869104
    11. VRK1 is a novel chromatin component that reacts to its alterations and participates very early in DNA damage response, functioning by itself or in cooperation with Ataxia Telangiectasia Mutated Proteins. PMID: 25923214
    12. VRK1 deficiency in human and mouse leads to downregulation of amyloid-beta precursor protein (APP). APP overexpression rescues the phenotype caused by Vrk1 knockdown, suggesting that VRK1 affects neuronal migration through an APP-dependent mechanism. PMID: 25609612
    13. VRK1 can contribute to make these tumors more resistant to DNA damage-based therapies, such as ionizing radiation or doxorubicin, which is consistent with its association to a poor prognosis in ER positive breast cancer. PMID: 24731990
    14. VRK1 deficiency disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes. PMID: 24430874
    15. The VRK1-p53 basal complex is an early-warning system for immediate cellular responses to DNA damage. PMID: 24492002
    16. This study identification of disease-causing alleles in 3 children from 2 unrelated families with Mutations in VRK1 associated with complex motor and sensory axonal neuropathy plus microcephaly. PMID: 24126608
    17. Data from molecular dynamic simulations suggest that bivalent cations play key structural roles in stabilization of VRK1/VRK2; bivalent cations have no obvious effects on VRK3. These kinases are targets in search for antineoplastic agents. [REVIEW] PMID: 23082977
    18. MKP2 is a negative regulator of VRK1-mediated histone H3 phosphorylation. PMID: 23223570
    19. Data show that vaccinia-related kinase 1 (VRK1) in resting cells plays an important role in the formation of ionizing radiation-induced foci that assemble on the 53BP1 scaffold protein during the DNA damage response. PMID: 22621922
    20. direct interaction and binding features between VRK1 and macroH2A1.2 PMID: 22194607
    21. VRK1 interacts and phosphorylates coilin, a protein regulating the assembly of Cajal bodies in the nucleus. PMID: 21920476
    22. NMR solution structure of a catalytically active form of human VRK1 PMID: 21543316
    23. VRK1 downregulation is necessary to modulate the block in cell cycle progression induced by p53 as part of its DNA damage response. PMID: 21386980
    24. vrk1 gene mutation can be excluded in 2 Chinese pedigrees of the first and second branchial arch syndrome. PMID: 18257281
    25. can perform the functions of B1 kinase required for vaccinia virus genome replication, most likely due to overlapping specificity for cellular and/or viral substrates PMID: 14747564
    26. VRK1 phosphorylates ATF2 and is an element of a novel signaling pathway that regulates gene transcription. PMID: 15105425
    27. c-Jun is phosphorylated by the human vaccinia-related kinase 1 (VRK1) and cooperates with the N-terminal kinase of c-Jun (JNK) PMID: 15378002
    28. VRK1 is the first step in a new pathway regulating p53 activity during cell proliferation PMID: 15542844
    29. VRK1 might play a role in cell cycle regulation and is likely to represent the beginning of a new control mechanism of cell cycle, particularly late in the G1-S phase. PMID: 16547155
    30. As the amount of p53 protein increases, there is a downregulation of the VRK1 protein level independent of its promoter. PMID: 16782868
    31. The different reactivity of the VRK1 protein indicates that this protein has a subcellular localization that can be regulated, thus adding an additional level of regulatory complexity to the VRK1 protein. PMID: 17617371
    32. accumulation of VRK1 in tumours with mutant p53 could result in stimulation of other signalling pathways PMID: 17689819
    33. These findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals. PMID: 17938195
    34. Small interference RNAs specific for Vrk1, was identified that reproducibly reduce viral RNA and viral protein levels in hepatitis C virus replicon-bearing cells. PMID: 17951261
    35. VRK1 is an immediate early response gene required for entry in G1 PMID: 18286197
    36. the downregulation of VRK1 protein levels, as a consequence of p53 accumulation, is thus dependent on the levels of the p300/CBP protein available for transcriptional complexes PMID: 18612383
    37. Ran is a novel negative regulator of nuclear VRK1 and VRK2 kinase activity, which may vary in different subcellular localizations generating an asymmetric intracellular distribution of kinase activity depending on local protein interactions. PMID: 18617507
    38. Results show that VRK1 activates CREB and regulates cell cycle progression in the DNA replication period by inducing cyclin D1 (CCND1) expression. PMID: 18713830
    39. The Plk3-VRK1 kinase module might represent two consecutive steps of a signaling cascade that participates in the regulation of Golgi fragmentation. PMID: 19103756
    40. Spinal muscular atrophy with pontocerebellar hypoplasia is caused by a mutation in the VRK1 gene. PMID: 19646678

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  • 相关疾病：
    Pontocerebellar hypoplasia 1A (PCH1A)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • 蛋白家族：
    Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
  • 组织特异性：
    Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • 数据库链接：

    HGNC: 12718

    OMIM: 602168

    KEGG: hsa:7443

    STRING: 9606.ENSP00000216639

    UniGene: Hs.422662