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  4. Recombinant Human Serine/threonine-protein kinase Nek2 (NEK2)

Recombinant Human Serine/threonine-protein kinase Nek2 (NEK2)

  • 中文名称：
    人NEK2重组蛋白
  • 货号：
    CSB-YP015699HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NEK2重组蛋白
  • 货号：
    CSB-EP015699HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NEK2重组蛋白
  • 货号：
    CSB-EP015699HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NEK2重组蛋白
  • 货号：
    CSB-BP015699HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NEK2重组蛋白
  • 货号：
    CSB-MP015699HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NEK2
  • Uniprot No.：
  • 别名：
    HSPK 21; HsPK21; NEK 2; NEK2; NEK2_HUMAN; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a) related kinase 2; NimA like protein kinase 1; NIMA related kinase 2; NimA related protein kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK 1; NLK1; Serine/threonine-protein kinase Nek2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-445
  • 氨基酸序列
    MPSRAEDYEV LYTIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEAEKQ MLVSEVNLLR ELKHPNIVRY YDRIIDRTNT TLYIVMEYCE GGDLASVITK GTKERQYLDE EFVLRVMTQL TLALKECHRR SDGGHTVLHR DLKPANVFLD GKQNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQMNR MSYNEKSDIW SLGCLLYELC ALMPPFTAFS QKELAGKIRE GKFRRIPYRY SDELNEIITR MLNLKDYHRP SVEEILENPL IADLVADEQR RNLERRGRQL GEPEKSQDSS PVLSELKLKE IQLQERERAL KAREERLEQK EQELCVRERL AEDKLARAEN LLKNYSLLKE RKFLSLASNP ELLNLPSSVI KKKVHFSGES KENIMRSENS ESQLTSKSKC KDLKKRLHAA QLRAQALSDI EKNYQLKSRQ ILGMR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction.; Phosphorylates and activates NEK11 in G1/S-arrested cells.; Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.
  • 基因功能参考文献：
    1. Taken together with the result that gamma-taxilin protein expression levels were decreased at the onset of mitosis, we propose that gamma-taxilin participates in Nek2A-mediated centrosome disjunction as a negative regulator through its interaction with Nek2A. PMID: 29225051
    2. NEK2 can promote HCC metastasis through induction of the epithelial-mesenchymal transition. PMID: 29399700
    3. evaluated the role of NEK2 in bone destruction of MM via clinical gene expression profiling data analysis and in vitro and in vivo mouse models. Further, treatment with the heparanase inhibitor Roneparstat, a Phase 1 drug in MM treatment,14 markedly inhibited OCL differentiation and rescued bone mass loss induced by NEK2 overexpression, suggesting that targeting NEK2 and/or its signaling pathway may be not only for preven PMID: 28400617
    4. Nek2 is able to individually shorten cilia when cilia are growing but requires interaction with Aurora A when cilia are being absorbed. PP1 binding protein, PPP1R42, directly inhibits Nek2 and increases cilia formation. PMID: 29141582
    5. Data suggest that low NIMA-related kinase 2 (NEK2) expression might be a useful predictor in hepatocellular carcinoma (HCC) as a poor prognostic factor, and could serve as a potential therapeutic target for HCC. PMID: 28759960
    6. present study firstly reveals that NEK2 protein is widely overexpressed in gliomas. NEK2 overexpression correlates significantly with malignancy (WHO grades), proliferation (Ki-67) and prognosis in malignant gliomas PMID: 28321704
    7. Results found NEK2 to be upregulated in lung cancer tissues and, its 3'-UTR mRNA is directly targeted by miR-128 to regulate its protein expression. PMID: 28514100
    8. Displacement of Dishevelled (DVL) from the centrosome and its release into the cytoplasm on NEK2 phosphorylation is coupled to the removal of linker proteins. PMID: 27486244
    9. NEK2 promotes aerobic glycolysis through regulating splicing of PKM and increasing the PKM2/PKM1 ratio in myeloma cells which contributes to its oncogenic activity. PMID: 28086949
    10. Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein. PMID: 27297360
    11. NEK2 has a role in maintaining glioma stem cells in glioblastoma by stabilizing the EZH2 protein PMID: 28737508
    12. This study reports a novel set of Dengue virus (DENV) NS1-interacting host cell proteins in the HepG2 cell line and proposes possible roles for human NEK2, TAO1, and COG1 in DENV infection. PMID: 27108190
    13. Our findings suggested that NIMA-related kinase 2B could play a role in the development and progression of breast cancer. Combination treatment using NIMA-related kinase 2B small interfering RNA and paclitaxel might be a novel potential therapy method for breast cancer. PMID: 28475000
    14. NEK2 modulates hepatoma cell functions, including growth, drug resistance, metastasis and angiogenesis via downstream genes activation PMID: 27925179
    15. Alteration of NEK2 protein levels may contribute to invasion and metastasis of hepatocellular carcinoma PMID: 28101574
    16. the functional roles of NEK2 in cancer development (review) PMID: 27019372
    17. NEK2 was associated with unfavorable outcomes in HCC patients. PMID: 27349376
    18. enhanced NEK2 expression in hepatocellular carcinoma (HCC) promotes HCC progression and drug resistance by promoting PP1/Akt and Wnt pathway activation, which may represent a new therapeutic target for HCC. PMID: 27509921
    19. Our findings demonstrated that NEK2 could be a valuable carcinogenic factor and a promising therapeutic target for primary liver cancer PMID: 26337275
    20. Suggest elevated Nek2 protein expression may be an independent risk factor for colon cancer. PMID: 26823916
    21. Hepatocellular carcinoma patients with high NEK2 expression had significantly poor recurrence-free survival (p=0.042) and early recurrence after hepatectomy. PMID: 26851035
    22. Cep85 is a bona fide Nek2A-binding partner that surrounds the proximal ends of centrioles where it cooperates with PP1gamma (also known as PPP1CC) to antagonize Nek2A PMID: 26220856
    23. Kif24 is a physiological substrate of Nek2, which regulates cilia disassembly through a concerted mechanism involving Kif24-mediated microtubule depolymerization. PMID: 26290419
    24. The results suggest that overexpression of NEK2 has the potential to serve as a biomarker for PCa prognosis. PMID: 26320076
    25. Nek2A is degraded in mitosis regardless of enforced spindle checkpoint activation PMID: 25673878
    26. we highlight recent findings on the aberrant expression and functional significance of NEK2A in human cancers and emphasize their significance for therapeutic potentials. PMID: 25705694
    27. Findings are consistent with the hypothesis that NEK2 contributes to ALDH1A1-dependent drug resistance in myeloma PMID: 25230277
    28. Nek2 was demonstrated to phosphorylate Cep68 in vivo and this phosphorylation appears to promote Cep68 degradation in mitosis. PMID: 25704143
    29. Nek2 plays a central role in proteasome-mediated cell cycle regulation and in conferring resistance to bortezomib in cancer cells. PMID: 25313354
    30. High expression of Nek2 could serve as an independent predictor of poor prognosis in pancreatic ductal adenocarcinoma. PMID: 24966957
    31. Overexpression of the Nek2 kinase is associated with colorectal cancer. PMID: 25043295
    32. Data suggest a role for Nek2 driving centrosome amplification downstream of the E2F3 activator. PMID: 24797070
    33. These results identify a novel mechanism for regulating beta-catenin stability that is independent of GSK3beta and provide new insight into a pathway involving Plk1, Nek2, and beta-catenin that regulates the centrosome cycle. PMID: 24501426
    34. Nek2 may represent a new potential target for non-small cell lung cancer therapeutic intervention PMID: 24763826
    35. it appears that Nek2 and Plk4 might synergize to promote breast tumorigenesis and may also be involved in tamoxifen and trastuzumab resistance PMID: 24389189
    36. NEK2 directly or indirectly interacts with a number of genes, proteins, microRNAs and biological processes associated with drug resistance in ovarian and other types of cancer. PMID: 24337664
    37. Up-regulation of NEK2 by microRNA-128 methylation is associated with colorectal cancer. PMID: 24046120
    38. Nek2 overexpression could not induce centrosome amplification and chromosome instability without expression of TRF1. PMID: 24091727
    39. these data indicate that Nek2 has a pivotal role in breast cancer growth at primary and secondary sites, and thus may be an attractive and novel therapeutic target for this disease. PMID: 23708664
    40. CIP2A strongly interacts with NEK2 during G2/M phase, thereby enhancing NEK2 kinase activity to facilitate centrosome separation in a PP1- and PP2A-independent manner. PMID: 24214971
    41. NEK2 as a novel p53-repressed gene PMID: 24163369
    42. These results suggest that the malignant transformation of plexiform neurofibroma is associated with distinct changes in the expression of BUB1B, PBK and NEK2 PMID: 23370767
    43. Data conclude that centrosome amplification is modulated through Cdk4 and Nek2 signaling and that binucleation is a likely source of CA in Her2+ breast cancer cells. PMID: 23776583
    44. Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene. PMID: 24043777
    45. NEK2 phosphorylation antagonizes the microtubule stabilizing activity of centrobin. PMID: 23291182
    46. small interfering RNA and antisense oligonucleotides against Nek2 worked synergistically with paclitaxel and doxorubicin by promoting cell apoptosis. PMID: 23340795
    47. The recruitment of Nek2 to the proximal ends of centrioles is dependent on one of its substrates, the centrosome cohesion protein C-Nap1. PMID: 22613497
    48. Our results indicate that genetic polymorphisms of centrobin and Nek2 are related to breast cancer susceptibility in Chinese Han women. PMID: 23001753
    49. Nek2 C downregulation, using RNA interference, decreased the survival, invasion and migration of MCF10DCIS.com and MCF10CA1a cells as well as in human primary breast cancer tissue. PMID: 22824957
    50. Nek2A binds with high affinity to apo-APC/C and is degraded by the pool of Cdc20 that avoids inhibition by the spindle assembly checkpoint. PMID: 23288039

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  • 相关疾病：
    Retinitis pigmentosa 67 (RP67)
  • 亚细胞定位：
    [Isoform 1]: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; [Isoform 2]: Cytoplasm. Note=Predominantly cytoplasmic.; [Isoform 4]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
  • 数据库链接：

    HGNC: 7745

    OMIM: 604043

    KEGG: hsa:4751

    STRING: 9606.ENSP00000355966

    UniGene: Hs.153704