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  4. Recombinant Human Serine-threonine kinase receptor-associated protein (STRAP)

Recombinant Human Serine-threonine kinase receptor-associated protein (STRAP)

  • 中文名称：
    人STRAP重组蛋白
  • 货号：
    CSB-EP896713HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    STRAP
  • Uniprot No.：
  • 别名：
    MAP activator with WD repeats; MAWD; PTWD; Serine-threonine kinase receptor-associated protein; serine/threonine kinase receptor associated protein; strap; STRAP_HUMAN; UNR-interacting protein; UNRIP; WD 40 repeat protein PT WD; WD-40 repeat protein PT-WD
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    65.4kDa
  • 表达区域：
    1-350aa
  • 氨基酸序列
    MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
  • 基因功能参考文献：
    1. these results suggest that STRAP increases invasion and metastasis of colorectal cancer partly through inhibiting ubiquitin-dependent degradation of beta-catenin and promoting Wnt/beta-catenin signaling PMID: 26910283
    2. Findings illuminated a novel STRAP-NOTCH1-HES1 molecular axis as a CSC regulator in colorectal cancer. PMID: 28827371
    3. MAWD and MAWBP were downregulated and associated with the differentiation grade in GC tissues. PMID: 26373288
    4. STRAP inhibits the transactivation function of Sp1 either by directly blocking its DNA binding domain or destabilizing Sp1 protein through ubiquitin-proteasome pathway in cell cycle G1 phase. PMID: 25483064
    5. Strap augments the apoptotic effects of mitochondrial p53. PMID: 25168243
    6. Coexpression of MAWBP and MAWD inhibited epithelial mesenchymal transformation, and EMT-aided malignant cell progression was suppressed in gastric cancer. PMID: 23687415
    7. Data indicate serine-threonine kinase receptor-associated protein (STRAP) as a novel regulator of the coordinated translation of collagen alpha1(I) and alpha2(I) mRNAs. PMID: 23918805
    8. both of the TPR and OB-fold domains localize to the chromatin of p53 target genes and exhibit intrinsic regulatory activity necessary for the Strap-dependent p53 response PMID: 22362889
    9. STRAP protein decreases ubiquitination of intracellular domain of Notch3 and may help to stabilize it. PMID: 21502811
    10. B-MYB acts as a positive regulator of STRAP PMID: 21148321
    11. gonucleotide pulldown identified STRAP (serine/threonine kinase receptor-associated protein) as a further protein recruited to the MMP28 promoter and acting functionally with Sp1. PMID: 20144149
    12. STRAP can act as a negative regulator of ASK1. PMID: 19880523
    13. unrip functions in the pathway of small nuclear ribonucleoproteins biogenesis PMID: 15848170
    14. biochemical evidence for the existence of another, yet atypical, SMN complex component, termed unr-interacting protein (unrip) PMID: 16159890
    15. STRAP acts as an intermediate signaling molecule linking between the phosphatidylinositol 3-kinase/PDK1 and the TGF-beta signaling pathways PMID: 16251192
    16. Gemin8 has an essential role in the proper structural organization of the SMN complex and the involvement of the heteromeric subunit containing Gemin6, Gemin7, Gemin8, and Unrip in the recruitment of Sm proteins to the snRNP assembly pathway PMID: 17023415
    17. the direct interaction of NM23-H1 and STRAP is important for the regulation of TGF-beta-dependent biological activity as well as NM23-H1 activity PMID: 17314099
    18. These results indicate that upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. PMID: 17316412
    19. an interplay between PPM1G and unrip determine compartment-specific phosphorylation patterns, localization, and function of the SMN complex PMID: 17984321
    20. Strap regulation reflects the coordinated interplay between different DNA damage-activated protein kinases, ATM and Chk2 (Checkpoint kinase 2), where phosphorylation by each kinase provides a distinct functional consequence on the activity of Strap. PMID: 18833288

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Localized predominantly in the cytoplasm but also found in the nucleus.
  • 蛋白家族：
    WD repeat STRAP family
  • 数据库链接：

    HGNC: 30796

    OMIM: 605986

    KEGG: hsa:11171

    STRING: 9606.ENSP00000392270

    UniGene: Hs.743971