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  4. Recombinant Human Rho guanine nucleotide exchange factor 25 (ARHGEF25)

Recombinant Human Rho guanine nucleotide exchange factor 25 (ARHGEF25)

  • 中文名称：
    Recombinant Human Rho guanine nucleotide exchange factor 25(ARHGEF25),Yeast
  • 货号：
    CSB-YP803131HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Rho guanine nucleotide exchange factor 25(ARHGEF25),Yeast
  • 货号：
    CSB-EP803131HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Rho guanine nucleotide exchange factor 25(ARHGEF25),Yeast
  • 货号：
    CSB-EP803131HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Rho guanine nucleotide exchange factor 25(ARHGEF25),Yeast
  • 货号：
    CSB-BP803131HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Rho guanine nucleotide exchange factor 25(ARHGEF25),Yeast
  • 货号：
    CSB-MP803131HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ARHGEF25
  • Uniprot No.：
  • 别名：
    ARHGEF25; ARHGP_HUMAN; GEFT ; Guanine nucleotide exchange factor GEFT; p63RhoGEF; RAC/CDC42 exchange factor; Rac/Cdc42/Rho exchange factor GEFT; Rho guanine nucleotide exchange factor 25; RhoA/RAC/CDC42 exchange factor; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-580
  • 氨基酸序列
    MRGGHKGGRC ACPRVIRKVL AKCGCCFARG GRESYSIAGS EGSISASAAS GLAAPSGPSS GLSSGPCSPG PPGPVSGLRR WLDHSKHCLS VETEADSGQA GPYENWMLEP ALATGEELPE LTLLTTLLEG PGDKTQPPEE ETLSQAPESE EEQKKKALER SMYVLSELVE TEKMYVDDLG QIVEGYMATM AAQGVPESLR GRDRIVFGNI QQIYEWHRDY FLQELQRCLK DPDWLAQLFI KHERRLHMYV VYCQNKPKSE HVVSEFGDSY FEELRQQLGH RLQLNDLLIK PVQRIMKYQL LLKDFLKYYN RAGMDTADLE QAVEVMCFVP KRCNDMMTLG RLRGFEGKLT AQGKLLGQDT FWVTEPEAGG LLSSRGRERR VFLFEQIIIF SEALGGGVRG GTQPGYVYKN SIKVSCLGLE GNLQGDPCRF ALTSRGPEGG IQRYVLQAAD PAISQAWIKH VAQILESQRD FLNALQSPIE YQRRESQTNS LGRPRGPGVG SPGRIQLGDQ AQGSTHTPIN GSLPSLLLSP KGEVARALLP LDKQALGDIP QAPHDSPPVS PTPKTPPCQA RLAKLDEDEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
  • 基因功能参考文献：
    1. p63RhoGEF(619) relocates to the plasma membrane upon activation of Galphaq coupled GPCRs, resembling the well-known activation mechanism of RhoGEFs activated by Galpha12/13. PMID: 27833100
    2. p63RhoGEF can rapidly activate RhoA through endogenous G-protein coupled receptors. PMID: 26435194
    3. assessed p63RhoGEF gene and protein expression and RhoA/Rho kinase activity in essential hypertensive and Bartter's and Gitelman's syndrome patients, a model opposite to hypertension PMID: 24356540
    4. Signaling efficiency of Galphaq through its effectors p63RhoGEF and GEFT depends on their subcellular location. PMID: 23884432
    5. p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells. PMID: 23380069
    6. Signaling through p63RhoGEF provides a novel mechanism for selective regulation of blood pressure. PMID: 21885830
    7. p63RhoGEF is regulated chiefly through allosteric control by Galpha(q), as opposed to other known Galpha-regulated RhoGEFs, which are instead sequestered in the cytoplasm, perhaps because of their high basal activity. PMID: 21832057
    8. Specifically mediates angiotensin II- Gq/11-dependent RhoA activation and downstream signaling and processes in vascular smooth muscle cells PMID: 20739613
    9. Results show that p63RhoGEF is a RhoA-specific GEF that may play a key role in actin cytoskeleton reorganization in different tissues, especially in heart cellular morphology. PMID: 11861769
    10. GEFT enhances dendritic spine formation and neurite outgrowth in primary neurons and neuroblastoma cells, respectively, through the activation of Rac/Cdc42-PAK signaling pathways. PMID: 15322108
    11. a new signaling pathway by which G alpha(q/11)-coupled receptors specifically induce Rho signaling through a direct interaction of activated G alpha(q/11) subunits with p63RhoGEF. PMID: 15632174
    12. Endogenous GEFT is transcriptionally upregulated during myogenic differentiation and downregulated during adipogenic differentiation. PMID: 16314529
    13. Together, our data provide new evidence suggesting that GEFT is an important regulator of multiple processes involved in axon and dendrite formation. PMID: 16496360
    14. the crystal structure of the Galphaq-p63RhoGEF-RhoA complex, detailing the interactions of Galphaq with the Dbl and pleckstrin homology (DH and PH) domains of p63RhoGEF was determined PMID: 18096806
    15. MLK3 functions in a regulated way to limit levels of the activated GTPase Rho by binding to the Rho activator, p63RhoGEF/GEFT, which, in turn, prevents its activation by Galphaq. PMID: 18851832

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  • 亚细胞定位：
    Cell membrane. Cytoplasm, myofibril, sarcomere. Note=Highly colocalizes with actin regions.
  • 组织特异性：
    Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
  • 数据库链接：

    HGNC: 30275

    OMIM: 610215

    KEGG: hsa:115557

    STRING: 9606.ENSP00000335560

    UniGene: Hs.61581