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  4. Recombinant Human Regulation of nuclear pre-mRNA domain-containing protein 1B (RPRD1B)

Recombinant Human Regulation of nuclear pre-mRNA domain-containing protein 1B (RPRD1B)

  • 中文名称：
    人RPRD1B重组蛋白
  • 货号：
    CSB-YP878854HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RPRD1B重组蛋白
  • 货号：
    CSB-EP878854HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RPRD1B重组蛋白
  • 货号：
    CSB-EP878854HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RPRD1B重组蛋白
  • 货号：
    CSB-BP878854HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RPRD1B重组蛋白
  • 货号：
    CSB-MP878854HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RPRD1B
  • Uniprot No.：
  • 别名：
    RPRD1B; C20orf77; CREPT; Regulation of nuclear pre-mRNA domain-containing protein 1B; Cell cycle-related and expression-elevated protein in tumor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-326
  • 氨基酸序列
    MSSFSESALE KKLSELSNSQ QSVQTLSLWL IHHRKHAGPI VSVWHRELRK AKSNRKLTFL YLANDVIQNS KRKGPEFTRE FESVLVDAFS HVAREADEGC KKPLERLLNI WQERSVYGGE FIQQLKLSME DSKSPPPKAT EEKKSLKRTF QQIQEEEDDD YPGSYSPQDP SAGPLLTEEL IKALQDLENA ASGDATVRQK IASLPQEVQD VSLLEKITDK EAAERLSKTV DEACLLLAEY NGRLAAELED RRQLARMLVE YTQNQKDVLS EKEKKLEEYK QKLARVTQVR KELKSHIQSL PDLSLLPNVT GGLAPLPSAG DLFSTD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
  • 基因功能参考文献：
    1. Aberrant overexpression of CREPT contributes to tumorigenesis of colorectal cancer by promoting cell proliferation and accelerating the cell cycle, and confers sensitivity to 5-fluorouracil. PMID: 29398868
    2. Ccisplatin-induced peripheral neuropathy(isIPN) is associated with age, modifiable risk factors, and genetically determined expression level of RPRD1B Further study of implicated genes could elucidate the pathophysiologic underpinnings of CisIPN. PMID: 28611204
    3. CREPT is closely relevant to the proliferation of NSCLC cells. PMID: 29397041
    4. Altogether, our results provided a novel insight into CREPT in regulating gastric cancer progression through apoptosis regulated by ROS/p53 pathways. PMID: 29402413
    5. Mechanistically, CREPT regulated beta-catenin/TCF4/cyclin D1 pathway in BC. In conclusion, the data suggested that miR-138/CREPT involved BC progression, providing potential therapeutic targets for BC. PMID: 28893536
    6. Consistently, samples from oral squamous cell carcinoma (OSCC) patients exhibited a noticeably stronger CREPT expression than noncancerous samples. In contrast, knocking down of CREPT in OSCC cell lines significantly reduced proliferation, colony formation and migration as well as the expression of cyclin D1 and c-Myc, but promoted apoptosis. PMID: 28369091
    7. In addition, CREPT overexpression significantly promoted tumor growth in vivo. Mechanism study showed that CREPT may regulate cell proliferation and cell cycle through the regulation on cyclin D3, CDK4 and CDK6. PMID: 27773816
    8. a detailed description of proteins associating with K-H/RPRD1B in higher-order protein complexes is required to further elucidate its role in various cellular processes PMID: 26819409
    9. CREPT displays unique immunostaining for retroperitoneal leiomyosarcoma tissue and can be used to supplement other currently available markers. PMID: 25400738
    10. study suggests that CREPT acts as an activator to promote transcriptional activity of the beta-catenin.TCF4 complex in response to Wnt signaling. PMID: 24982424
    11. RPRD1B overexpression promotes tumor growth and accelerates cell cycle progression. PMID: 24452636
    12. This study reports that K-H functions in RNAPII regulation, and aids in stabilizing interactions between transcription termination factors, localizing Xrn2 to the 3'-end of genes and ultimately suppressing R-loop formation. PMID: 24589584
    13. CREPT increases cyclin D1 transcription during tumorigenesis. PMID: 22264791

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    UPF0400 (RTT103) family
  • 组织特异性：
    Preferentially expressed in a range of tumor tissues including colon, lung, liver, breast, prostate, stomach, uterine endometrium and cervical cancers with higher levels in tumors than in adjacent non-tumor tissue (at protein level).
  • 数据库链接：

    HGNC: 16209

    OMIM: 614694

    KEGG: hsa:58490

    STRING: 9606.ENSP00000362532

    UniGene: Hs.278839