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  4. Recombinant Human Rab-interacting lysosomal protein (RILP)

Recombinant Human Rab-interacting lysosomal protein (RILP)

  • 中文名称：
    人RILP重组蛋白
  • 货号：
    CSB-YP850399HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RILP重组蛋白
  • 货号：
    CSB-EP850399HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RILP重组蛋白
  • 货号：
    CSB-EP850399HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RILP重组蛋白
  • 货号：
    CSB-BP850399HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RILP重组蛋白
  • 货号：
    CSB-MP850399HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RILP
  • Uniprot No.：
  • 别名：
    FLJ31193; PP10141; Rab interacting lysosomal protein; Rab-interacting lysosomal protein; Rilp; RILP_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MEPRRAAPGV PGWGSREAAG SASAAELVYH LAGALGTELQ DLARRFGPEA AAGLVPLVVR ALELLEQAAV GPAPDSLQVS AQPAEQELRR LREENERLRR ELRAGPQEER ALLRQLKEVT DRQRDELRAH NRDLRQRGQE TEALQEQLQR LLLVNAELRH KLAAMQTQLR AAQDRERERQ QPGEAATPQA KERARGQAGR PGHQHGQEPE WATAGAGAPG NPEDPAEAAQ QLGRPSEAGQ CRFSREEFEQ ILQERNELKA KVFLLKEELA YFQRELLTDH RVPGLLLEAM KVAVRKQRKK IKAKMLGTPE EAESSEDEAG PWILLSDDKG DHPPPPESKI QSFFGLWYRG KAESSEDETS SPAPSKLGGE EEAQPQSPAP DPPCSALHEH LCLGASAAPE A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes.
  • 基因功能参考文献：
    1. Hepatitis C virus (HCV) modifies cellular trafficking by cleaving Rab interacting lysosomal protein (RILP), which serves to redirect ras-related protein Rab-7 (Rab7)-containing vesicles to a kinesin-dependent trafficking mode promoting virion secretion. PMID: 27791088
    2. RILP suppresses the invasion of breast cancer cells by interacting with RalGDS to inhibit its guanine nucleotide exchange factor activity for RalA. PMID: 26469971
    3. RILP regulates vacuolar ATPase through interaction with the V1G1 subunit PMID: 26180254
    4. VPS41 subunit of HOPS complex was defined to be the major partner for interacting with RILP. PMID: 25445562
    5. RILP regulates the activity of the V-ATPase through its interaction with V1G1. PMID: 24762812
    6. RILP directly and concomitantly binds the tethering HOPS complex and the p150(Glued) subunit of the dynein motor. ORP1L then functions as a cholesterol-sensing switch controlling RILP-HOPS-p150(Glued) interactions. PMID: 23729732
    7. Unique region in RILP responsible for its specific role in regulating lysosomal morphology as well as in its interaction with Rab7 and Rab34. PMID: 14668488
    8. The crystal structure of Rab7-GTP in complex with the Rab7 binding domain of RILP reveals that Rab7 interacts with RILP specifically via two distinct areas. PMID: 15933719
    9. The data together indicate that RILP, as already demonstrated for several other Rab effector proteins, is capable of self-association, thus probably forming a homo-dimer. PMID: 15996637
    10. RILP indeed prenylated, while phosphorylation analysis showed that the two protein kinase A sites are phosphorylated. PMID: 16417580
    11. Hence, RILPsv provides an extra dimension to the control of vesicle fusion and transport by the small GTPase Rab7. PMID: 16631113
    12. we have isolated, using the yeast two-hybrid system, the EAP30/SNF8/VPS22 subunit of the ESCRT-II complex as a RILP interacting protein. PMID: 16857164
    13. These results establish that RILP is required for biogenesis of multivesicular endosomes and degradative trafficking of EGFRs but not for trafficking of transferrin receptors through early endosomes. PMID: 17959629
    14. REST/NRSF, dynactin p150(Glued), huntingtin, HAP1, and RILP form a complex involved in the translocation of REST/NRSF into the nucleus and HAP1 controls REST/NRSF cellular localization in neurons PMID: 18922795
    15. Results describe how ORP1L contacts VAP to control Rab7-RILP-p150 Glued and late endosome positioning. PMID: 19564404

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  • 亚细胞定位：
    Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle, phagosome membrane.
  • 组织特异性：
    Ubiquitous. Strongly expressed in fetal heart, heart, stomach, spleen, adrenal gland, thyroid gland, salivary gland, fetal liver, liver and lung. Poorly expressed in brain.
  • 数据库链接：

    HGNC: 30266

    OMIM: 607848

    KEGG: hsa:83547

    STRING: 9606.ENSP00000301336

    UniGene: Hs.534497