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  4. Recombinant Human RAD51-associated protein 1 (RAD51AP1)

Recombinant Human RAD51-associated protein 1 (RAD51AP1)

  • 中文名称：
    人RAD51AP1重组蛋白
  • 货号：
    CSB-YP850261HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RAD51AP1重组蛋白
  • 货号：
    CSB-EP850261HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RAD51AP1重组蛋白
  • 货号：
    CSB-EP850261HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RAD51AP1重组蛋白
  • 货号：
    CSB-BP850261HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RAD51AP1重组蛋白
  • 货号：
    CSB-MP850261HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD51AP1
  • Uniprot No.：
  • 别名：
    2510006L10Rik; MGC156698; OTTMUSP00000028651; PIR51; R51A1_HUMAN; RAB22; RAD51 associated protein 1; RAD51 interacting protein; RAD51-associated protein 1; RAD51-interacting protein; Rad51ap1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-352
  • 氨基酸序列
    MVRPVRHKKP VNYSQFDHSD SDDDFVSATV PLNKKSRTAP KELKQDKPKP NLNNLRKEEI PVQEKTPKKR LPEGTFSIPA SAVPCTKMAL DDKLYQRDLE VALALSVKEL PTVTTNVQNS QDKSIEKHGS SKIETMNKSP HISNCSVASD YLDLDKITVE DDVGGVQGKR KAASKAAAQQ RKILLEGSDG DSANDTEPDF APGEDSEDDS DFCESEDNDE DFSMRKSKVK EIKKKEVKVK SPVEKKEKKS KSKCNALVTS VDSAPAAVKS ESQSLPKKVS LSSDTTRKPL EIRSPSAESK KPKWVPPAAS GGSRSSSSPL VVVSVKSPNQ SLRLGLSRLA RVKPLHPNAT ST
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination. Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination. May also bind RNA; additional evidences are however required to confirm RNA-binding in vivo.
  • 基因功能参考文献：
    1. RAD51AP1 upregulation is described in both tissue and matching blood from patients with ovarian and lung cancer compared with healthy controls. PMID: 29126163
    2. The current understanding of the biochemical and biological functions of RAD51AP1 as HR factor is reviewed. It is also discussed how RAD51AP1 may help to promote cancer development and potentially be a promising new target for therapeutic intervention. [REVIEW] PMID: 28963981
    3. USP1-UAF1 complex promotes homologous recombination repair via multiple mechanisms: through FANCD2 deubiquitination, as well as by interacting with RAD51AP1. PMID: 27463890
    4. RAD51AP1 is required for the maintenance of DNA replication fork progression. PMID: 25288561
    5. Data suggest that microRNA/mRNA pairs in hsa-miR-140-3p/RAD51AP1/, hsa-miR-145/E2F3, hsa-miR-139-5p/TOP2A, and hsa-miR-133a/GCLC were correlated with ovarian tumorigenesis. PMID: 22452920
    6. We have finely mapped the two DNA binding domains in RAD51AP1 and using mutant variants, reveal that both domains are indispensable for RAD51AP1 function in cells. PMID: 22375013
    7. RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif. PMID: 21903585
    8. RAD51AP1 foci colocalize with a subset of DMC1 foci in spermatocytes. PMID: 21307306
    9. Although PALB2 alone stimulates D-loop formation, it has a cooperative effect with RAD51AP1, an enhancer of RAD51. PMID: 20871616
    10. expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues PMID: 14966907
    11. RAD51AP1 has a selective affinity for branched-DNA structures that are obligatory intermediates during joint molecule formation. PMID: 17996710
    12. RAD51AP1 helps maintain genomic integrity via RAD51 recombinase enhancement. PMID: 17996711
    13. Enhanced expression of RAD51 associating protein-1 is involved in the growth of intrahepatic cholangiocarcinoma PMID: 18316552

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  • 亚细胞定位：
    Chromosome. Nucleus. Chromosome, telomere.
  • 组织特异性：
    Highly expressed in testis and thymus. Lower levels in colon and small intestine. Little or no expression in spleen, prostate, ovary and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 16956

    OMIM: 603070

    KEGG: hsa:10635

    STRING: 9606.ENSP00000228843

    UniGene: Hs.730696