Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Protein kinase C-binding protein NELL1 (NELL1)

Recombinant Human Protein kinase C-binding protein NELL1 (NELL1)

  • 中文名称：
    人NELL1重组蛋白
  • 货号：
    CSB-YP846105HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NELL1重组蛋白
  • 货号：
    CSB-EP846105HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NELL1重组蛋白
  • 货号：
    CSB-EP846105HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NELL1重组蛋白
  • 货号：
    CSB-BP846105HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NELL1重组蛋白
  • 货号：
    CSB-MP846105HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NELL1
  • Uniprot No.：
  • 别名：
    FLJ45906; IDH3GL; NEL like 1 (chicken); NEL like protein 1; Nel related protein 1; NEL-like 1 (chicken); NEL-like protein 1; Nel-related protein 1; NELL 1 protein short isoform; Nell1; NELL1_HUMAN; Neural epidermal growth factor like 1; NRP1; Protein kinase C binding protein NELL1; Protein kinase C-binding protein NELL1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-810
  • 氨基酸序列
    FGMDPDLQM DIVTELDLVN TTLGVAQVSG MHNASKAFLF QDIEREIHAA PHVSEKLIQL FRNKSEFTIL ATVQQKPSTS GVILSIRELE HSYFELESSG LRDEIRYHYI HNGKPRTEAL PYRMADGQWH KVALSVSASH LLLHVDCNRI YERVIDPPDT NLPPGINLWL GQRNQKHGLF KGIIQDGKII FMPNGYITQC PNLNHTCPTC SDFLSLVQGI MDLQELLAKM TAKLNYAETR LSQLENCHCE KTCQVSGLLY RDQDSWVDGD HCRNCTCKSG AVECRRMSCP PLNCSPDSLP VHIAGQCCKV CRPKCIYGGK VLAEGQRILT KSCRECRGGV LVKITEMCPP LNCSEKDHIL PENQCCRVCR GHNFCAEGPK CGENSECKNW NTKATCECKS GYISVQGDSA YCEDIDECAA KMHYCHANTV CVNLPGLYRC DCVPGYIRVD DFSCTEHDEC GSGQHNCDEN AICTNTVQGH SCTCKPGYVG NGTICRAFCE EGCRYGGTCV APNKCVCPSG FTGSHCEKDI DECSEGIIEC HNHSRCVNLP GWYHCECRSG FHDDGTYSLS GESCIDIDEC ALRTHTCWND SACINLAGGF DCLCPSGPSC SGDCPHEGGL KHNGQVWTLK EDRCSVCSCK DGKIFCRRTA CDCQNPSADL FCCPECDTRV TSQCLDQNGH KLYRSGDNWT HSCQQCRCLE GEVDCWPLTC PNLSCEYTAI LEGECCPRCV SDPCLADNIT YDIRKTCLDS YGVSRLSGSV WTMAGSPCTT CKCKNGRVCC SVDFECLQNN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
  • 基因功能参考文献：
    1. Results show that NELL1 methylation level was 6.8% higher in renal cell cancer (RCC) tissues associated with advanced disease of distant metastases, and shorter RFS. Analyses suggested that NELL1 DNA methylation is a promising candidate prognostic epigenetic biomarker for more detailed analyses of RCC disease. PMID: 30272321
    2. This work indicates that NELL-1, HMGB1, and CCN2 might enhance bone defect healing via the recruitment of endogenous cells and induction of vascularization and act via different processes than BMP2. PMID: 28463604
    3. NELL1 variant is associated with tobacco- and HPV-mediated oral oncogenesis. PMID: 30091681
    4. Results demonstrate that NELL1 is differentially expressed in fusion-positive alveolar rhabdomyosarcoma (ARMS) and in embryonal rhabdomyosarcoma (ERMS) samples, and they show that this transcriptional difference partially depends on genomic DNA methylation. The study also found that high NELL1 levels are correlated with negative RMS prognostic factors and with poor tumor outcomes. PMID: 28380437
    5. lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-beta3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair. PMID: 26700847
    6. establish the feasibility of combining NELL-1 with BMP2 to improve clinical bone regeneration and provide mechanistic insight into canonical Wnt pathway activity during NELL-1 and BMP2 osteogenesis PMID: 26772960
    7. Data suggest that TSPN (N-terminal thrombospondin-1-like) domain of NELL1 exhibits major heparin-binding sites which may be involved in interaction of NELL1 with cell surface heparan sulfate proteoglycans. PMID: 26627376
    8. NELL-1 signaling activates Wnt/beta-catenin signaling. PMID: 26082355
    9. We also detected lower relative expression of Nell-1 by real-time PCR. Furthermore, immunohistochemical analyses revealed that Nell-1 staining was less intense in cancer tissue relative to normal tissue and that the tumor cells had spread to the muscle PMID: 26090379
    10. CpG islands in the NELL1 and NELL2 promoters are hypermethylated in renal cell carcinoma.NELL1 and NELL2 protein expression is downregulated in clear cell renal carcinoma. PMID: 25726761
    11. NELL-1 demonstrates diffuse and reliable expression in benign but not malignant bone-forming skeletal tumors PMID: 25791475
    12. NELL1 overexpression greatly enhanced the osteogenic differentiation and mineral synthesis of iPSC-MSCs on RGD-grafted CPC scaffold for the first time. PMID: 25220281
    13. Two single-nucleotide polymorphisms of the NELL1 gene may represent a novel mechanism underlying hydrochlorothiazide-induced adverse metabolic effects (meta-analysis). PMID: 23400010
    14. NELL1 is able to promote the osteogenic differentiation of periodontal ligament stem cells, which may be related to the downregulation of Msx2 expression. PMID: 22767336
    15. Activation of the JNK pathway is necessary to mediate terminal osteogenic different-iation of Saos-2 osteosarcoma cells by rhNELL-1. PMID: 22797704
    16. Data indicate that LvNELL1 infection promoted the osteogenic differentiation of hADSCs, and the effect was comparable with that of LvBMP2. PMID: 23017834
    17. SHH and NELL-1 directed signaling produced additive effects on the pro-osteogenic and antiadipogenic differentiation of adipose derived stem cells. PMID: 22264144
    18. Prediction of radiographic severity in Ankolysing Spondylitis based on clinical variables can be significantly improved by including SNPs at ADRB1 (rs1801253), NELL1 (rs8176785) and MHC (rs1634747, rs9270986, rs7451962 and rs241453) genes. PMID: 22495925
    19. These results identify one potential mechanism of action for rhNELL-1 induced osteogenesis and highlight a fundamental difference between NELL-1 and BMP-2 signaling. PMID: 22580275
    20. NELL-1 may produce functional cartilage with properties similar to native cartilage. PMID: 21902605
    21. Results suggest that Osterix is a direct transcriptional regulator with repressive effect on NELL-1 gene expression, contributing to a delicate balance of regulatory effects on NELL-1 transcription with Runx2. PMID: 21931789
    22. Nell-1 is a candidate growth factor able to induce pericyte osteogenic differentiation. PMID: 21615216
    23. the effects of NELL-1 on osteoblastic differentiation and proliferation are partly through binding to APR3 PMID: 21723284
    24. The osteogenic effects of NELL1 on femoral distraction osteogenesis, was investigated. PMID: 20959151
    25. a potent growth factor that is highly specific to the osteochondral lineage, and has demonstrated robust induction of bone in multiple in vivo models [review] PMID: 20647499
    26. Data indicate that NELL1 is a ubiquitous inflammatory bowel disease susceptibility locus. PMID: 17684544
    27. Runx2 directly binds to the osteoblast specific binding elements 2 elements and transactivates the human NELL-1 promoter. PMID: 17042739
    28. Promoter hypermethylation of NELL1 is a common, tissue-specific event in human EAC, occurs early during Barrett's-associated esophageal neoplastic progression, and is a potential biomarker of poor prognosis in early-stage EAC PMID: 17452981
    29. findings suggest that upon binding to a specific receptor NELL1 transduces an osteogenic signal through activation of certain Tyr-kinases associated with the Ras-MAPK cascade, and finally leads to the osteogenic differentiation PMID: 18082140
    30. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
    31. Transgenic mice overexpressing Nell-1 develop craniosynostosis. PMID: 12235118

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Nucleus envelope. Secreted. Note=Colocalizes with ATRAID on the nuclear envelope and the perinuclear region.
  • 数据库链接：

    HGNC: 7750

    OMIM: 602319

    KEGG: hsa:4745

    STRING: 9606.ENSP00000349654

    UniGene: Hs.657172