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  4. Recombinant Human Protein NDNF (NDNF)

Recombinant Human Protein NDNF (NDNF)

  • 货号：
    CSB-YP823447HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP823447HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP823447HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP823447HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP823447HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NDNF
  • Uniprot No.：
  • 别名：
    C4orf31; Fibronectin type III domain containing protein C4orf31; NDNF; NDNF_HUMAN; Neuron-derived neurotrophic factor; PRO6487; Protein NDNF; UNQ2748
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-568
  • 氨基酸序列
    Q KLPTRDEELF QMQIRDKAFF HDSSVIPDGA EISSYLFRDT PKRYFFVVEE DNTPLSVTVT PCDAPLEWKL SLQELPEDRS GEGSGDLEPL EQQKQQIINE EGTELFSYKG NDVEYFISSS SPSGLYQLDL LSTEKDTHFK VYATTTPESD QPYPELPYDP RVDVTSLGRT TVTLAWKPSP TASLLKQPIQ YCVVINKEHN FKSLCAVEAK LSADDAFMMA PKPGLDFSPF DFAHFGFPSD NSGKERSFQA KPSPKLGRHV YSRPKVDIQK ICIGNKNIFT VSDLKPDTQY YFDVFVVNIN SNMSTAYVGT FARTKEEAKQ KTVELKDGKI TDVFVKRKGA KFLRFAPVSS HQKVTFFIHS CLDAVQIQVR RDGKLLLSQN VEGIQQFQLR GKPKAKYLVR LKGNKKGASM LKILATTRPT KQSFPSLPED TRIKAFDKLR TCSSATVAWL GTQERNKFCI YKKEVDDNYN EDQKKREQNQ CLGPDIRKKS EKVLCKYFHS QNLQKAVTTE TIKGLQPGKS YLLDVYVIGH GGHSVKYQSK VVKTRKFC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    重组人源NDNF（NADH脱氢酶复合物装配因子）蛋白，它在细胞内的功能与线粒体呼吸链复合物的装配和维护有关。下面是重组人NDNF的一般步骤：
    设计和合成基因：基于人NDNF的氨基酸序列，设计并合成含有NDNF基因的DNA序列。这可以通过基因合成公司或PCR方法完成。
    克隆基因：将NDNF基因插入到表达载体中，这可以通过PCR扩增和限制性内切酶切割的方法完成。建议选择一些经过验证的表达载体，如pET系列载体，这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    转染宿主细胞：在细胞中转染含有NDNF基因的表达载体。这些宿主细胞可以选择常见的哺乳动物细胞系，如CHO细胞、HEK293细胞等。
    培养和表达蛋白：根据表达载体中的启动子和调节元件，诱导NDNF蛋白在转染的宿主细胞中表达。在培养期间，需要添加抗生素等来保持表达载体的稳定。
    蛋白质的纯化：通过细胞破碎和离心等方法提取含有NDNF蛋白的细胞裂解液。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的NDNF蛋白进行鉴定和定量。此外，可以使用酶活性分析等方法来检测其生物学活性和功能。
    需要注意的是，线粒体蛋白的表达和纯化需要特殊的条件和方法。因此，在进行NDNF的表达和纯化时，需要注意线粒体蛋白的稳定性和功能性，避免蛋白质的降解和失活。
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Secretory protein that plays a role in various cellular processes. Acts as a chemorepellent acting on gonadotropin-releasing hormone (GnRH) expressing neurons regulating their migration to the hypothalamus. Also promotes neuron migration, growth and survival as well as neurite outgrowth and is involved in the development of the olfactory system. May also act through the regulation of growth factors activity and downstream signaling. Also regulates extracellular matrix assembly and cell adhesiveness. Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms.
  • 基因功能参考文献：
    1. NDNF acts as an endogenous modulator that promotes endothelial cell function and ischemia-induced revascularization through eNOS-dependent mechanisms PMID: 24706764
    2. direct duplication of chromosome 4q27q31.3 de novo results in partial trisomy of its long arm. PMID: 16970039
  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Expressed in neurons along the gonadotropin-releasing hormone (GnRH) expressing neurons migratory route.
  • 数据库链接：

    HGNC: 26256

    OMIM: 616506

    KEGG: hsa:79625

    STRING: 9606.ENSP00000369014

    UniGene: Hs.709520