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  4. Recombinant Human Protein DBF4 homolog A (DBF4)

Recombinant Human Protein DBF4 homolog A (DBF4)

  • 货号：
    CSB-YP887010HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP887010HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP887010HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP887010HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP887010HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Activator of S phase kinase; ASK; CHIF; Chiffon homolog A; DBF4; DBF4 homolog (S. cerevisiae); DBF4 homolog; DBF4 type zinc finger containing protein 1; DBF4 zinc finger A; DBF4 zinc finger; DBF4; S. cerevisiae; homolog of; DBF4-type zinc finger-containing protein 1; DBF4A; DBF4A_HUMAN; Dumbbell former 4; S. cerevisiae; homolog of; OTTHUMP00000161661; Protein DBF4 homolog A; ZDBF1; Zinc finger; DBF-type containing 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-674
  • 氨基酸序列
    MNSGAMRIHS KGHFQGGIQV KNEKNRPSLK SLKTDNRPEK SKCKPLWGKV FYLDLPSVTI SEKLQKDIKD LGGRVEEFLS KDISYLISNK KEAKFAQTLG RISPVPSPES AYTAETTSPH PSHDGSSFKS PDTVCLSRGK LLVEKAIKDH DFIPSNSILS NALSWGVKIL HIDDIRYYIE QKKKELYLLK KSSTSVRDGG KRVGSGAQKT RTGRLKKPFV KVEDMSQLYR PFYLQLTNMP FINYSIQKPC SPFDVDKPSS MQKQTQVKLR IQTDGDKYGG TSIQLQLKEK KKKGYCECCL QKYEDLETHL LSEQHRNFAQ SNQYQVVDDI VSKLVFDFVE YEKDTPKKKR IKYSVGSLSP VSASVLKKTE QKEKVELQHI SQKDCQEDDT TVKEQNFLYK ETQETEKKLL FISEPIPHPS NELRGLNEKM SNKCSMLSTA EDDIRQNFTQ LPLHKNKQEC ILDISEHTLS ENDLEELRVD HYKCNIQASV HVSDFSTDNS GSQPKQKSDT VLFPAKDLKE KDLHSIFTHD SGLITINSSQ EHLTVQAKAP FHTPPEEPNE CDFKNMDSLP SGKIHRKVKI ILGRNRKENL EPNAEFDKRT EFITQEENRI CSSPVQSLLD LFQTSEEKSE FLGFTSYTEK SGICNVLDIW EEENSDNLLT AFFSSPSTST FTGF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • 基因功能参考文献：
    1. Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies. PMID: 28448802
    2. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
    3. we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA. PMID: 27407105
    4. The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells. PMID: 24287290
    5. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. PMID: 22123827
    6. bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. PMID: 21536671
    7. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
    8. Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle. PMID: 19744312
    9. The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK. PMID: 19864417
    10. results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site PMID: 12015319
    11. The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245. PMID: 12420215
    12. Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase. PMID: 12535533
    13. Cdc7-Dbf4 kinase efficiently phosphorylates p150. PMID: 16826239
    14. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
    15. Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation. PMID: 17276990
    16. There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma. PMID: 17768177
    17. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
    18. ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1. PMID: 18503552
    19. A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified. PMID: 18536724
    20. increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies PMID: 18714392
    21. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • 数据库链接：

    HGNC: 17364

    OMIM: 604281

    KEGG: hsa:10926

    STRING: 9606.ENSP00000265728

    UniGene: Hs.485380