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  4. Recombinant Human Probable ATP-dependent RNA helicase DDX53 (DDX53)

Recombinant Human Probable ATP-dependent RNA helicase DDX53 (DDX53)

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  • 中文名称：
    Recombinant Human Probable ATP-dependent RNA helicase DDX53(DDX53)
  • 货号：
    CSB-EP773024HU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP773024HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DDX53.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP773024HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DDX53.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    DDX53
  • Uniprot No.：
  • 别名：
    CAGE; Cancer associated gene protein; Cancer-associated gene protein; Cancer/testis antigen 26; CT26; DDX53; DDX53_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 53; DEAD box protein 53; DEAD box protein CAGE; Probable ATP dependent RNA helicase DDX53; Probable ATP-dependent RNA helicase DDX53
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    76.2 kDa
  • 表达区域：
    1-631aa
  • 氨基酸序列
    MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant human DDX53 protein is a fusion protein consists of the human DDX53 protein (1-631aa) partnered with the N-terminal 10xHis tag and C-terminal Myc tag. It was produced in the E.coli. This recombinant DDX53 protein's purity is greater than 85% determined by SDS-PAGE. After electrophoresis, there is a 78 kDa protein band presented on the gel.

    Human Probable ATP-dependent RNA helicase DDX53 functions as an oncogene and increases the level of cyclins. Aberrant expression of DDX53 has been documented in various cancer tissues and cell lines. Helicases are implicated in most stages of the gene expression pathway, ranging from DNA replication, RNA transcription, splicing, RNA transport, ribosome biogenesis, mRNA translation, RNA storage and decay. Besides, methylation has an important role for the expression regulation of DDX53. It has been found that DDX53 confers resistance to various anti-cancer drugs. Furthermore, a study noted that DDX53 promotes cancer stem cell-like properties and autophagy. DDX53 showed a co-expression with CD133, and binding to SOX-2, a marker of cancer stemness. Threrfore, DDX53 has been regared as a novel role of DDX53 in regulating cancer stem cell-like properties. Additionally, Activation of DDX53 was observed in a variety of tumors, including gastric and endometrial cancers, as well as in hematological cancers.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. A novel role of DDX53 in regulating cancer stem cell-like properties by binding to SOX-2.DDX53 directly regulates the SOX-2 expression in drug-resistant melanoma cells. PMID: 28535666
    2. DDX53 promotes stem cell-like properties, autophagy, and confers resistance to anti-cancer drugs in breast cancer cells. PMID: 28152297
    3. Results provide evidence that miR-30a forms a positive feedback loop with CAGE to regulate the expression of p53 confering resistance to anti-cancer drugs. PMID: 26912082
    4. miR-217-CAGE feedback loop serves as a target for overcoming resistance to various anti-cancer drugs, including EGFR and HER2 inhibitors. PMID: 26863629
    5. Results show the direct regulation of CAGE expression by HDAC3 and that HDAC3-CAGE axis regulates the activation of EGFR. HDAC3 targets CAGE to regulate the tumorigenic potential and angiogenic potential of cancer cells. PMID: 26883907
    6. CAGE induced the interaction between histone deacetylase 2 (HDAC2) and Snail, which exerted a negative effect on p53 expression in neoplastic cells; CAGE confers drug resistance by regulating expression of p53 through HDAC2. PMID: 20534591
    7. CAGE is expressed in a variety of cancers but not in normal tissues except testis, and might play a role in cellular proliferation. PMID: 11922625
    8. results suggest that the methylation status of the CpG sites of CAGE determines its expression, and that hypomethylation of CAGE precedes the development of gastric cancer and hepatocellular carcinoma PMID: 12849980
    9. CAGE promotes motility of cancer cells through activation of focal adhesion kinase (FAK). PMID: 17028776
    10. Hypomethylated CAGE promoter CpG islands are frequently involved in uterine cervical carcinogenesis. PMID: 17341616
    11. The CAGE gene is widely expressed in various cancer tissues and cancer cell lines. PMID: 18388483

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DEAD box helicase family
  • 组织特异性：
    Expressed in testis. Wide expression in various cancer tissues and cancer cell lines.
  • 数据库链接：

    HGNC: 20083

    KEGG: hsa:168400

    STRING: 9606.ENSP00000368667

    UniGene: Hs.434416